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Molecular identification of the ATP-release Maxi-Cl channel and its roles in ischemia-reperfusion cardiac injury

Research Project

Project/Area Number 26293045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General physiology
Research InstitutionNational Institute for Physiological Sciences (2017)
The Graduate University for Advanced Studies (2014-2016)

Principal Investigator

Okada Yasunobu  生理学研究所, 名誉教授 (10025661)

Co-Investigator(Kenkyū-buntansha) 岡田 俊昭  生理学研究所, 生体機能調節研究領域, 特任准教授 (00373283)
松浦 博  滋賀医科大学, 医学部, 教授 (60238962)
沼田 かお理 (佐藤かお理 / 沼田 かお理(佐藤かお理))  生理学研究所, 大学共同利用機関等の部局等, 研究員 (60614196)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsアニオンチャネル / 心臓 / ATP放出 / 虚血・再灌流 / 左心室発生圧 / アニオンチャンネル
Outline of Final Research Achievements

Cellular release of ATP is a key event in purinergic signaling in animal tissues. We showed previously that the ATP release is mediated by Maxi-Cl in many cell types including cardiomyocytes. Here, we have identified SLCO2A1, which is known to be a prostaglandin transporter (PGT), as the pore component of Maxi-Cl. Also, we have shown that SLCO2A1 is involved in swelling-induced ATP release from Maxi-Cl-rich C127 cells. When Langendorff-perfused mouse hearts were subjected to oxygen-glucose deprivation (OGD), ATP was released to the coronary effluent upon reperfusion. This OGD-induced heart ATP release was suppressed by in vivo pre-injection of SLCO2A1-siRNA or by application of a PGT blocker, indicating a SLCO2A1 involvement in the OGD-induced cardiac ATP release. This ATP release was associated with increased left ventricular developed pressure, in a manner sensitive to an adenosine A1 receptor antagonist, suggesting a protective role of released ATP via A1 receptor signaling.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (27 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 5 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] ウズベキスタン科学アカデミー生物有機化学研究所(ウズベキスタン)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ウズベキスタン国立アカデミー生物有機化学研究所(ウズベキスタン)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The organic anion transporter SLCO2A1 constitutes the core component of the Maxi-Cl channel.2017

    • Author(s)
      Ravshan Z. Sabirov, Petr.G. Merzlyak, Toshiaki Okada, Md. Rafiqul Islam, Hiromi Uramoto, Tomoko Mori, Yumiko Makino, Hiroshi Matsuura, Yu Xie & Yasunobu. Okada
    • Journal Title

      The EMBO J.

      Volume: 36 Issue: 22 Pages: 3309-3324

    • DOI

      10.15252/embj.201796685

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Specific and essential but not sufficient roles of LRRC8A in the activity of volume-sensitive outwardly rectifying anion channel (VOSR).2017

    • Author(s)
      T. Okada, Md.R. Islam, N.A. Tsiferova, Y. Okada & R.Z. Sabirov
    • Journal Title

      Channels (Austin)

      Volume: 11 Issue: 2 Pages: 109-120

    • DOI

      10.1080/19336950.2016.1247133

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel2017

    • Author(s)
      Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Ravshan Z. Sabirov and Yasunobu Okada.
    • Journal Title

      Channels

      Volume: 11(2) Issue: 2 Pages: 167-172

    • DOI

      10.1080/19336950.2016.1230574

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Channeling frozen cells to survival after thawing: opening the door to cryo-physiology.2016

    • Author(s)
      Y. Okada
    • Journal Title

      J. Physiol. (London)

      Volume: 594 Issue: 6 Pages: 1523-1524

    • DOI

      10.1113/jp271842

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Biophysics and physiology of the volume-regulated anion channel (VRAC)/volume-sensitive outwardly rectifying anion channel (VSOR).2016

    • Author(s)
      S.F. Pedersen, Y. Okada & B. Nilius
    • Journal Title

      Pflugers Arch. Eur. J. Physiol.

      Volume: 468 Issue: 3 Pages: 371-383

    • DOI

      10.1007/s00424-015-1781-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The properties, functions and pathophysiology of maxi-anion channels2016

    • Author(s)
      Sabirov RZ, Merzlyak PG, Islam Md R, Okada T & Okada Y
    • Journal Title

      Pflugers Arch. Eur. J. Physiol.

      Volume: 468 Issue: 3 Pages: 405-420

    • DOI

      10.1007/s00424-015-1774-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] OPINION:バックグラウンドからフォアフロントへ:細胞生死と生体恒常性に関わるセンサーチャネル(後編その3)2015

    • Author(s)
      岡田泰伸
    • Journal Title

      日本生理学雑誌

      Volume: 77 Pages: 15-18

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Temperature sensitivity of acid-sensitive outwardly rectifying (ASOR) anion channels in cortical neurons is involved in hypothermic neuroprotection against acidotoxic necrosis.2014

    • Author(s)
      K. Sato-Numata, T. Numata & Y. Okada
    • Journal Title

      Channels

      Volume: 8 Pages: 1-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A newly cloned ClC-3 isoform, ClC-3d, as well as ClC-3a mediates Cd^<2+>-sensitive outwardly rectifying anion currents.2014

    • Author(s)
      岡田俊昭、秋田天平、佐藤(沼田)かお理、Md. Rafiqul Islam、岡田泰伸
    • Journal Title

      Cellular Physiology and Biochemistry

      Volume: 33 Issue: 3 Pages: 539-556

    • DOI

      10.1159/000358633

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characteristics and roles of the volume-sensitive outwardly rectifying (VSOR) anion channel in the central nervous system.2014

    • Author(s)
      T. Akita & Y. Okada
    • Journal Title

      Neuroscience

      Volume: 275 Pages: 211-231

    • DOI

      10.1016/j.neuroscience.2014.06.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 生から死へ、死から生への生理学 -統合科学序説-2014

    • Author(s)
      岡田泰伸
    • Journal Title

      総合研究大学院大学葉山彙報

      Volume: 5 Pages: 31-51

    • Related Report
      2014 Annual Research Report
  • [Presentation] SLCO2A1 is a pore-forming component of Maxi-Cl channel.2018

    • Author(s)
      Okada T, Islam Md R, Merzlyak GP, Sabirov RZ, Okada Y
    • Organizer
      第95回 日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SLCO2A1 is involved in a pathway for ATP release from cultured cells and Langendorff-perfused hearts.2018

    • Author(s)
      Islam Md R, Okada T, Matsuura H, Uramoto H, Sabirov RZ, Okada Y
    • Organizer
      第95回 日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Properties and roles of flufenamate-sensitive ion channels stimulated by hyperosmolality in vasopressin neurons.2018

    • Author(s)
      Sato-Numata K, Numata T, Ueda Y, Inoue R, Okada Y
    • Organizer
      第95回 日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular identification of two types of volume-activated anion channels involved in multiple functions controlling cell life.2017

    • Author(s)
      Okada Y, Sabirov RZ, Okada T, Merzlyak PG, Islam Md R, Sato-Numata K, Uramoto H, Ando-Akatsuka Y, Matsuura H
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of the key molecule of Maxi-Cl channel by proteomics and genome-wide approaches.2017

    • Author(s)
      Okada T, Sabirov RZ, Merzlyak PG, Islam Md R, Uramoto H, Mori T, Makino Y, Okada Y
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular verification for the Maxi-Cl channel molecule as a cellular ATP release pathway.2017

    • Author(s)
      Islam Md R, Merzlyak PG, Okada T, Uramoto H, Okada Y, Sabirov RZ
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      浜松
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Properties of the maxi-anion channel in melanoma cells.2016

    • Author(s)
      Tsiferova NA, Merzlyak PG, Okada Y,Sabirov RZ
    • Organizer
      第93回 日本生理学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evidence for a modulatory role of annexin A2 in the maxi-anion channel activity.2015

    • Author(s)
      Islam Md R, Okada T, Merzlyak P, Sabirov RZ, Okada Y
    • Organizer
      第8回アジアオセアニア生理学会大会
    • Place of Presentation
      タイ国 バンコック市
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Annexin A2 is a modulator of maxi-anion channel (Maxi-Cl) in mouse mammary C127 cells.2015

    • Author(s)
      Islam Md R, Okada T, Merzlyak PG, Sabirov RZ, Okada Y
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Exploration of temperature sensitivity and new antagonists of acid-sensitive outwardly rectifying anion channel (ASOR).2015

    • Author(s)
      Sato-Numata K, Numata T, Okada Y
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Examination of the role of LRRC8A in the function of volume-sensitive outwardly rectifying anion channel (VSOR).2015

    • Author(s)
      Okada T, Sato-Numata K, Islam Md R, Sabirov RZ, Okada Y
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Volume-sensitive anion channels in melanoma cells before and after tumor formation.2015

    • Author(s)
      Sabirov RZ, Tsiferova NA, Merzlyak PG, Okada Y
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Book] “ギャノング 生理学”(原書24版)2014

    • Author(s)
      岡田泰伸(監訳者)
    • Total Pages
      838
    • Publisher
      丸善、東京
    • Related Report
      2014 Annual Research Report
  • [Remarks] 自然科学研究機構生理学研究所ホームページ

    • URL

      http://www.nips.ac.jp/release/2017/10/atp_2.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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