Studies on Siglecs expressed on B lymphocytes and their glycan ligands
| Project/Area Number |
26293062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TSUBATA Takeshi 東京医科歯科大学, 難治疾患研究所, 教授 (80197756)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | シグレック / CD22 / Siglec-10 シスリガンド / CD45 / シアル酸 / シグナル伝達 / ギラン・バレー症候群 / シスリガンド / BCR / 糖鎖 / 免疫学 / Bリンパ球 / Siglec |
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| Outline of Final Research Achievements |
Siglecs expressed in various immune cells recognize sialic acids and mostly inhibit cell activation. In this study, we demonstrated that sialic acid-containing Siglec ligands expressed on the same cell (cis-ligands) can be efficiently labeled by proximity labeling. We elucidated how cis-ligands regulate Siglecs by analyzing CD45 that was isolated as a cis-ligand of CD22, a Siglec. Moreover, we demonstrated that the polymorphism of Siglec-10 associated with Guillain-Barre syndrome in which autoantibodies to gangliosides, sialic acid-containing glycolipids, are produced regulates binding of Siglec-10 to gangliosides. This result suggests that decreased activity of Siglec-10 allows immune responses to gangliosides, resulting in development of this syndrome.
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Report
(5 results)
Research Products
(47 results)
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[Journal Article] CD22-binding synthetic sialosides regulate B lymphocyte proliferation through CD22 ligand-dependent and independent pathways, and enhances antibody production in mice2018
Author(s)
2.Matsubara N, Imamura A, Yonemiz Tu, Akatsu C, Yang H, Ueki A, Watanabe N, Abdu-Allah H, Numoto N, Takematsu H, Kitazume S, Tedder FT, Marth JD, Ito N, Ando H, Ishida H, Kiso M, and Tsubata T
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Journal Title
Front Immunol
Volume: -
Pages: 820-820
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] CD72 negatively regulates B lymphocyte responses to the lupus-related endogenous Toll-like receptor 7 ligand Sm/RNP.2016
Author(s)
Akatsu, C., Shinagawa, K., Numoto, N., Liu, Z., Konuscan, A.U., Aslam, M., Phoon, S., Adachi, T., Furukawa, K., Ito, N. and Tsubata. T.
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Journal Title
J. Exp. Med.
Volume: 213
Pages: 2691-2706
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Functional evaluation of activation-dependent alterations in the sialoglycan composition of T cells.2014
Author(s)
Naito-Matsui Y, Takada S, Kano Y, Iyoda T, Sugai M, Shimizu A, Inaba K, Nitschke L, Tsubata T, Oka S, Kozutsumi Y, and Takematsu H.
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Journal Title
J.Bio.Chem.
Volume: 289
Pages: 1564-1579
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] LAPTM5 promotes lysosomal degradation of intracellular but not the cell surface CD3ζimmunol..2014
Author(s)
Kawai, Y., Ouchida, R., Yamasaki, S., Dragone, L., Tsubata, T. and Wang, J.-Y.
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Journal Title
Immunol Cell Biol.
Volume: 92
Pages: 527-534
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] A mouse model for chronic inflammatory diseases spontaneously develops CD4/CD8 double-positive T cell leukemia/lymphoma.2015
Author(s)
P. A. Koni., Ren, M., Tsubata, T., Khleif, S., Maverakis, E. and Shimoda, M.
Organizer
The 54th Midwinter Conference of Immunologists at Asilomar.
Place of Presentation
Big Bear Lake, California (USA)
Year and Date
2015-01-24 – 2015-01-25
Related Report
Invited
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[Presentation] CD273＋memory B cells replenish bone marrow plasma cells in the steady state after influenza vaccination.2014
Author(s)
Onodera, T., Adachi, T., Tsubata, T., Kurosaki, T., Adachi, Y., Ato, M., Takahashi, Y.
Organizer
Annual Meeting of the Japanese Society for Immunology, 2014.
Place of Presentation
京都国際会館、Kyoto
Year and Date
2014-12-10 – 2014-12-12
Related Report
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