| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
To elucidate the molecular mechanisms of the selection and maturation of thymocytes, we have analyzed the role of calcium signaling in T lymphocytes using cell-type specific Stim1 and Stim2 doubly or calcineurin b1 knockout mice. Using Foxp3-IRES-YFP/Cre Tg mice, we found that the store-operated calcium entry－calcium signaling axis is not essential for maintenance of Foxp3 expression in regulatory T cells after maturation, but is necessary for their inhibitory function. Cell maintenance of iNKT cells after maturation was normal in the absence of store-operated calcium entry. These results indicate that calcium signaling positively regulates differentiation of agonist selected thymocytes, while it positively regulates cell functions rather than cell maintenance after maturation.
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