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Elucidation of the pathophysiological roles of thyroid hormone metabolism in diabetes mellitus

Research Project

Project/Area Number 26293125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionGunma University

Principal Investigator

Murakami Masami  群馬大学, 医学(系)研究科(研究院), 教授 (30241871)

Co-Investigator(Kenkyū-buntansha) 角野 博之  群馬大学, 医学部附属病院, 講師 (10375579)
常川 勝彦  群馬大学, 医学(系)研究科(研究院), 助教 (30436307)
奈良 誠人  群馬大学, 医学部附属病院, 助教 (80420165)
荒木 修  群馬大学, 医学部附属病院, 助教 (80589482)
木村 孝穂  群馬大学, 医学(系)研究科(研究院), 講師 (90396656)
荻原 貴之  群馬大学, 医学部附属病院, 助教 (80361377)
Co-Investigator(Renkei-kenkyūsha) OKU Hiroyuki  群馬大学, 理工学研究院, 准教授 (20301749)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords糖尿病 / 甲状腺ホルモン / 遺伝子 / 一塩基多型 / 自己抗体 / 動脈硬化
Outline of Final Research Achievements

We have studied the expression and regulation of thyroid hormone metabolism, including type 2 iodothyronine deiodinase (D2) which activates thyroid hormones and type 3 iodothyronine deiodinase (D3) which inactivates thyroid hormones.
We found the expression of D3 in cultured pancreatic beta cells, which is stimulated by GLP-1 via a cAMP-PKA mediated pathway. D3 is suggested to increases insulin secretion from pancreatic beta cells by decreasing intracellular T3. Furthermore, we discovered the expression of D2 in cultured human umbilical vein endothelial cells (HUVECs). Conversion of T4 to T3 by D2 is required for rapid nongenomic action of T4, which results in migration of HUVECs.
These results may open a novel perspective on understanding of the mechanisms of thyroid hormone action.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (48 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (7 results) Journal Article (19 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 19 results,  Open Access: 14 results,  Acknowledgement Compliant: 6 results) Presentation (15 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Book (6 results) Remarks (1 results)

  • [Int'l Joint Research] University of California Los Angeles/Vanderbilt University/Cornell University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Finsen Laboraotory/University of Copenhagen(デンマーク)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Montreal(カナダ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Universite de Lyon(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Cesar Milstein Hospital/INFIBIOC-University of Buenos Aires(Argentina)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Comparison of 16S ribosomal RNA gene sequence analysis and conventional culture in the environmental survey of a hospital.2017

    • Author(s)
      Manaka A, Tokue Y, Murakami M.
    • Journal Title

      J Pharm Health Care Sci.

      Volume: - Issue: 1

    • DOI

      10.1186/s40780-017-0074-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lipoprotein lipase does not increase significantly in the postprandial plasma.2017

    • Author(s)
      Ishiyama N, Sakamaki K, Shimomura Y, Kotani K, Tsuzaki K, Sakane N, Miyashita K, Fukamachi I, Kobayashi J, Stanhope KL, Havel PJ, Kamachi K, Tanaka A, Tokita Y, Machida T, Murakami M, Nakajima K.
    • Journal Title

      Clinica Chimica Acta

      Volume: 464 Pages: 204-210

    • DOI

      10.1016/j.cca.2016.11.035

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Triglyceride content in remnant lipoproteins is significantly increased after food intake and is associated with plasma lipoprotein lipase.2017

    • Author(s)
      Nakajima K, Tokita Y, Sakamaki K, Shimomura Y, Kobayashi J, Kamachi K, Tanaka A, Stanhope KL, Havel PJ, Wang T, Machida T, Murakami M.
    • Journal Title

      Clinica Chimica Acta

      Volume: 465 Pages: 45-52

    • DOI

      10.1016/j.cca.2016.12.011

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Monoclonal antibodies that bind to the Ly6 domain of GPIHBP1 abolish the binding of LPL.2017

    • Author(s)
      Hu X, Sleeman MW, Miyashita K, Linton MF, Allan CM, He C, Larsson M, Tu Y, Sandoval NP, Jung RS, Mapar A, Machida T, Murakami M, Nakajima K, Ploug M, Fong LG, Young SG, Beigneux AP.
    • Journal Title

      JOURNAL OF LIPID RESEARCH

      Volume: 58 Pages: 208-215

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Use of capillary electrophoresis with dual-opposite end injection for simultaneous analysis of small ions in saliva samples from wrestlers undergoing a weight training program.2016

    • Author(s)
      Mori M, Ishikawara F, Tomoda T, Yamada S, Okamoto M, Itabashi H, Seki Y, Matsumoto R, Shoho Y, Martha L, Sumino H, Murakami M.
    • Journal Title

      JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

      Volume: 15 Pages: 1012-1013

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The majority of lipoprotein lipase in plasma is bound to remnant lipoproteins: A new definition of remnant lipoproteins.2016

    • Author(s)
      Sato K, Okajima F, Miyashita K, Imamura S, Kobayashi J, Stanhope KL, Havel PJ, Machida T, Sumino H, Murakami M, Schaefer E, Nakajima K.
    • Journal Title

      Clin Chim Acta

      Volume: 461 Pages: 114-125

    • DOI

      10.1016/j.cca.2016.06.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An LPL-specific monoclonal antibody, 88B8, that abolishes the binding of LPL to GPIHBP1.2016

    • Author(s)
      Allan CM, Larsson M, Hu X, He C, Jung RS, Mapar A, Voss C, Miyashita K, Machida T, Murakami M, Nakajima K, Bensadoun A, Ploug M, Fong LG, Young SG, Beigneux AP
    • Journal Title

      JOURNAL OF LIPID RESEARCH

      Volume: 57 Issue: 10 Pages: 1889-1898

    • DOI

      10.1194/jlr.m070813

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biological Antioxidant Potential Negatively Correlates With Carotid Artery Intima-Media Thickness2016

    • Author(s)
      Yagi H, Sumino H, Yoshida K, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Nakajima K, Murakami M
    • Journal Title

      International Heart Journal

      Volume: 57 Issue: 2 Pages: 220-225

    • DOI

      10.1536/ihj.15-389

    • NAID

      130005139772

    • ISSN
      1349-2365, 1349-3299
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes.2016

    • Author(s)
      Yoshida K, Kimura T, Aoki T, Tsunekawa K, Araki O, Shoho Y, Nara M, Sumino H, Murakami M.
    • Journal Title

      J Int Med Res

      Volume: 44 Issue: 3 Pages: 496-507

    • DOI

      10.1177/0300060515627561

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Atherogenic lipoprotein in subclinical hypothyroidism and their relationship with hepatic lipase activity: response to replacement treatment with levothyroxine.2016

    • Author(s)
      Brenta G, Berg G, Miksztowics V, Lopez G, Lucero D, Faingold C, Murakami M, Machida T, Nakajima K, Schreier L.
    • Journal Title

      Thyroid

      Volume: 26 Issue: 3 Pages: 365-372

    • DOI

      10.1089/thy.2015.0140

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Type 2 Iodothyronine Deiodinase Activity Is Required for Rapid Stimulation of PI3K by Thyroxine in Human Umbilical Vein Endothelial Cells.2015

    • Author(s)
      Aoki T, Tsunekawa K, Araki O, Ogiwara T, Nara M, Sumino H, Kimura T, Murakami M.
    • Journal Title

      Endocrinology

      Volume: 156 Issue: 11 Pages: 4312-4324

    • DOI

      10.1210/en.2014-1988

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Impaired blood rheology is associated with endothelial dysfunction in patients with coronary risk factors.2015

    • Author(s)
      Yagi H, Sumino H, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Murakami M.
    • Journal Title

      Clin Hemorheol Microcirc.

      Volume: 62 Issue: 2 Pages: 139-150

    • DOI

      10.3233/ch-151960

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The role of circulating lipoprotein lipase and adiponectin on the particle size of remnant lipoproteins in patients with diabetes mellitus and metabolic syndrome2015

    • Author(s)
      Shirakawa T, Nakajima K, Yatsuzuka SI, Shimomura Y, Kobayashi J, Machida T, Sumino H, Murakami M
    • Journal Title

      Clinica Chimica Acta

      Volume: 440 Pages: 123-132

    • DOI

      10.1016/j.cca.2014.10.029

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism2015

    • Author(s)
      Shirakawa T, Nakajima K, Shimomura Y, Kobayashi J, Stanhope K, Havel P, Machida T, Sumino H, Murakami M
    • Journal Title

      Clinica Chimica Acta

      Volume: 440 Pages: 193-200

    • DOI

      10.1016/j.cca.2014.07.020

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between carotid artery intima-media thickness and small dense low-density lipoprotein cholesterol concentrations measured by homogeneous assay in Japanese subjects2015

    • Author(s)
      Aoki T, Yagi H, Sumino H, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Nakajima K, Murakami M
    • Journal Title

      Clin Chim Acta

      Volume: 442 Pages: 110-114

    • DOI

      10.1016/j.cca.2015.01.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer2015

    • Author(s)
      Machida T, Miyashita K, Sone T, Tanaka S, Nakajima K, Saito M, Stanhope K, Havel P, Sumino H, Murakami M
    • Journal Title

      Clin Chim Acta

      Volume: 442 Pages: 130-135

    • DOI

      10.1016/j.cca.2015.01.016

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Frequency and clinical implication of the R450H mutation in the thyrotropin receptor gene in the Japanese population detected by Smart Amplification Process 22014

    • Author(s)
      Tsunekawa K, Yanagawa Y, Aoki T, Morimura T, Araki O, Kimura T, Ogiwara T, Kotajima N, Yanagawa M, Murakami M
    • Journal Title

      Biomed Res Int

      Volume: 2014 Pages: 964635-964635

    • DOI

      10.1155/2014/964635

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glucagon-like peptide-1 stimulates type 3 iodothyronine deiodinase expression in a mouse isulinoma cell line2014

    • Author(s)
      Akiyama S, Ogiwara T, Aoki T, Tsunekawa K, Araki O, Murakami M
    • Journal Title

      Life Sci

      Volume: 115 Issue: 1-2 Pages: 22-28

    • DOI

      10.1016/j.lfs.2014.09.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Small dense LDL cholesterol measured by homogeneous assay in Japanese healthy controls, metabolic syndrome and diabetes patients with or without a fatty liver2014

    • Author(s)
      Kikkawa K, Nakajima K, Shimomura Y, Tokita Y, Machida T, Sumino H, Murakami M
    • Journal Title

      Clin Chim Acta

      Volume: 438 Pages: 70-79

    • DOI

      10.1016/j.cca.2014.07.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Assesment of the glucose tolerance among young Japanese subjects by the shape of plasma glucose concentration curve during OGTT2016

    • Author(s)
      Aoki T, Kimura T, Tsunekawa K, Araki O, Nara M, Yoshida A, Machida T, Murakami M
    • Organizer
      The 32nd World Congress of Biomedical Laboratory Science
    • Place of Presentation
      Kobe
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The sodium glucose co-transporter 2 receptor inhibitors attenuated the results leukocytes tested by urine dipsticks2016

    • Author(s)
      Kamiyama K, Machida T, Yoshida A, Araki O, Kimura T, Murakami M
    • Organizer
      The 32nd World Congress of Biomedical Laboratory Science
    • Place of Presentation
      Kobe
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of free and toral prostate-specific antigen assay on the AIA-CL2400 analyzer2016

    • Author(s)
      Iwai M, Nakajima K, Kamiyama K, Fujii S, Machida T, Araki O, Ito K, Murakami M
    • Organizer
      The 32nd World Congress of Biomedical Laboratory Science
    • Place of Presentation
      Kobe
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Changes in gender preference of female patients for repeated transthoracic echocardiography2016

    • Author(s)
      Okada K, Kurosawa K, Kimura T, Niwa K, Ikoma T, Morita K, Machida T, Murakami M
    • Organizer
      The 32nd World Congress of Biomedical Laboratory Science
    • Place of Presentation
      Kobe
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of the coagulation factor VIII and IX activity measurement using the chromogenic reagent2016

    • Author(s)
      Inoue M, Shizuka R, Sato A, Hayakawa M, Machida T, Tsunekawa K, Koiso H, Murakami M
    • Organizer
      The 32nd World Congress of Biomedical Laboratory Science
    • Place of Presentation
      Kobe
    • Year and Date
      2016-08-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Thyroid hormone action – update2016

    • Author(s)
      Murakami M, Aoki T, Tsunekawa K, Araki O, Nara M, Sumino H, Kimura T
    • Organizer
      第14回アジア臨床病理臨床検査医学会
    • Place of Presentation
      台湾・台北
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 臨床検査領域におけるグローバル化の推進 日本臨床検査医学会における国際化の取り組み2015

    • Author(s)
      村上正巳、山田俊幸
    • Organizer
      第62回日本臨床検査医学会学術集会
    • Place of Presentation
      岐阜県岐阜市
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ヒトオリゴデンドログリオーマ(HOG)細胞における甲状腺ホルモン代謝機構の解析2015

    • Author(s)
      荒木 修, 荻原 貴之, 石垣 宏尚, 牛木 和美, 正保 佳史, 青木 智之, 常川 勝彦, 木村 孝穂, 奈良 誠人, 角野 博之, 村上 正巳
    • Organizer
      第58回日本甲状腺学会学術集会
    • Place of Presentation
      福島県福島市
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 甲状腺刺激ホルモン(TSH)測定値のハーモナイゼーション2015

    • Author(s)
      竹岡 啓子, 日高 洋, 菱沼 昭, 池田 勝義, 大久保 滋夫, 土屋 達行, 橋口 照人, 堀田 多恵子, 古田 耕, 松下 一之, 松本 祐之, 村上 正巳, 前川 真人
    • Organizer
      第58回日本甲状腺学会学術集会
    • Place of Presentation
      福島県福島市
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] Current issues in laboratory medicine2015

    • Author(s)
      Murakami M
    • Organizer
      第14回インドネシア臨床病理臨床検査医学会
    • Place of Presentation
      インドネシア・マナド
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微量臨床検体におけるSmartAmp法の検出感度の解析2014

    • Author(s)
      正保 佳史, 常川 勝彦, 関口 拓磨, 青木 智之, 荒木 修, 木村 孝穂, 奈良 誠人, 荻原 貴之, 矢部 茂季, 町田 哲男, 村上 正巳
    • Organizer
      第61回日本臨床検査医学会
    • Place of Presentation
      福岡
    • Year and Date
      2014-11-22 – 2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト褐色脂肪と2型甲状腺ホルモン脱ヨード化酵素(DIO2)の一塩基多型2014

    • Author(s)
      米代 武司, 松下 真美, 正保 佳史, 常川 勝彦, 村上 正巳, 斉藤 昌之
    • Organizer
      第35回日本肥満学会
    • Place of Presentation
      宮崎
    • Year and Date
      2014-10-24 – 2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Clinical implication of aporipoprotein M: apolipoprotein M may be a biomarker of coagulation and fibrinolysis2014

    • Author(s)
      Aoki T, Kimura T, Shimizu T, Sumino H, Miyazaki O, Fukamachi I, Nakajima K, Okajima F, Murakami M
    • Organizer
      第82回欧州動脈硬化学会
    • Place of Presentation
      マドリッド
    • Year and Date
      2014-05-31 – 2014-06-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス膵β細胞由来MIN6細胞におけるGLP-1刺激による3型甲状腺ホルモン脱ヨード酵素(D3)の発現2014

    • Author(s)
      秋山 滋男, 荻原 貴之, 青木 智之, 常川 勝彦, 荒木 修, 村上 正巳
    • Organizer
      第57回日本糖尿病学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-22 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Association between the effect of physical exercise in glucose matabolism and the polymorphism of type 2 iodothyronine deiodinase2014

    • Author(s)
      Tsunekawa K, Aoki T, Araki O, Ogiwara T, Kimura T, Nara M, Sumino H, Machida T, Murakami M
    • Organizer
      The 9th Cherry Blossom Symposium           International Conference of Clinical Laboratory Automation and Robotics
    • Place of Presentation
      横浜
    • Year and Date
      2014-04-17 – 2014-04-19
    • Related Report
      2014 Annual Research Report
  • [Book] Thyroid Hormone Disruption and Neurodevelopment2016

    • Author(s)
      Murakami M
    • Total Pages
      209
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] 日常診療のための検査値のみかた2015

    • Author(s)
      村上正巳
    • Total Pages
      757
    • Publisher
      中外医学社
    • Related Report
      2014 Annual Research Report
  • [Book] 今日の臨床検査2015

    • Author(s)
      村上正巳
    • Total Pages
      673
    • Publisher
      南江堂
    • Related Report
      2014 Annual Research Report
  • [Book] これだけは知っておきたい検査のポイント2015

    • Author(s)
      村上正巳
    • Total Pages
      627
    • Publisher
      医学書院
    • Related Report
      2014 Annual Research Report
  • [Book] 医学大辞典第20版2015

    • Author(s)
      村上正巳
    • Total Pages
      3101
    • Publisher
      南山堂
    • Related Report
      2014 Annual Research Report
  • [Book] パーフェクトガイド検査値事典2014

    • Author(s)
      村上正巳
    • Total Pages
      707
    • Publisher
      総合医学社
    • Related Report
      2014 Annual Research Report
  • [Remarks] 甲状腺ホルモンによる血管修復のメカニズムを解明

    • URL

      http://www.gunma-u.ac.jp/information/8136

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

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