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Elucidation of hepatitis virus infection / replication mechanism using genome-modified human pluripotent stem cells

Research Project

Project/Area Number 26293178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionTokai University

Principal Investigator

KAMIYA Akihide  東海大学, 医学部, 准教授 (30321904)

Co-Investigator(Renkei-kenkyūsha) KAKINUMA Sei  東京医科歯科大学, 医学部, 准教授 (30372444)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
KeywordsヒトiPS細胞 / 肝炎ウイルス / 肝再生 / 肝前駆細胞 / 再生医学 / ウイルス / 幹細胞 / ウィルス
Outline of Final Research Achievements

Chronic infection of hepatitis B virus (HBV, HCV) is a major cause of cirrhosis, liver cancer etc in Japan. However, it was difficult to analyze because of the species specificity of its infectivity. In this study, for the purpose of deriving hepatocytes from human iPS cells and constructing an HBV infection analysis system, 1) preparation of highly expressed human hepatocytes of HBV receptors, 2) genes responsible for high function of hepatocytes Screening, etc. were conducted. As a result, it has been found that HBV is efficiently infected / amplified in human iPS cell-derived hepatocytes overexpressing Ntcp, and that KLF 15 is important for enhancing hepatocyte function.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(12 results)

All 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 8 results) Presentation (3 results)

  • [Journal Article] Characteristics of hepatic progenitor cells during liver development and regeneration.2017

    • Author(s)
      Kamiya A, Chikada H.
    • Journal Title

      Stem Cells in Clinical Applications “Liver, Lung and Heart Regeneration” (edited by Phuc Van Pham)

      Volume: 1 Pages: 3-13

    • DOI

      10.1007/978-3-319-46693-4_1

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Identification of a novel alpha-fetoprotein- expressing cell population induced by Jagged1/Notch2 signal in murine fibrotic liver.2017

    • Author(s)
      Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Minako Sueoka, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
    • Journal Title

      Hepatology Communications

      Volume: 印刷中 Pages: 1-15

    • DOI

      10.1002/hep4.1026

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Human induced pluripotent stem cell-derived hepatic cell line as a new model for host interaction with hepatitis B virus.2016

    • Author(s)
      Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Iwamoto M, Watashi K, Wakita T, Sugiyama M, Mizokami M, Shimotohno K, Watanabe M.
    • Journal Title

      Sci. Rep

      Volume: 6 Pages: 29358-29358

    • DOI

      10.1038/srep29358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Investigation of bi-potent differentiation of hepatoblasts using inducible diphtheria toxin receptor- transgenic mice.2016

    • Author(s)
      Yanagida A, Mizuno N, Yamazaki Y, Kato-Itoh M, Umino A, Sato H, Ito K, Yamaguchi T, Nakauchi H, Kamiya A.
    • Journal Title

      Hepatol Research

      Volume: Nov 19. Pages: 816-828

    • DOI

      10.1111/hepr.12622

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The basic helix-loop-helix transcription factor, Mist1, induces maturation of mouse fetal hepatoblasts.2015

    • Author(s)
      Chikada H, Ito K, Yanagida A, Nakauchi H, Kamiya A
    • Journal Title

      Scientific Reports

      Volume: 12 Pages: 14989-14989

    • DOI

      10.1038/srep14989

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] MEK-ERK activity regulates the proliferative activity of fetal hepatoblasts through accumulation of p16/19cdkn2a.2015

    • Author(s)
      Kamiya A, Ito K, Yanagida A, Chikada H, Iwama A, Nakauchi H
    • Journal Title

      Stem Cells and Developments

      Volume: 24 Pages: 2525-2535

    • DOI

      10.1089/scd.2015.0015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Liver maturation deficiency in p57Kip2-/- mice occurs in a hepatocytic p57Kip2 expression-independent manner.2015

    • Author(s)
      agida A, Chikada H, Ito K, Umino A, Kato-Itoh M, Yamazaki Y, Sato H, Kobayashi T, Yamaguchi T, Nakayama KI, Nakauchi H, Kamiya A.
    • Journal Title

      Dev. Biol.

      Volume: 407(2) Pages: 331-343

    • DOI

      10.1016/j.ydbio.2015.07.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A paracrine mechanism accelerating expansion of human induced pluripotent stem cell-derived hepatic progenitor-like cells2015

    • Author(s)
      Kota Tsuruya, Hiromi Chikada, Kinuyo Ida, Kazuya Anzai, Tatehiro Kagawa, Yutaka Inagaki, Tetsuya Mine, and Akihide Kamiya
    • Journal Title

      Stem Cells and Developments

      Volume: null Pages: 1691-1702

    • DOI

      10.1089/scd.2014.0479

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A paracrine mechanism accelerating expansion of human iPS cell-derived hepatic progenitor-like cells.2015

    • Author(s)
      Tsuruya K, Chikada H, Ida K, Anzai K, Kagawa Y, Inagaki Y, Mine T, and Kamiya A
    • Journal Title

      Stem Cell Dev.

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 肝前駆細胞の成熟化を誘導する転写調節機構2017

    • Author(s)
      紙谷聡英、安斎和也、鶴谷康太、近田裕美
    • Organizer
      第17回日本再生医療学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 肝前駆細胞由来胆管組織形成の分化制御メカニズム2016

    • Author(s)
      紙谷聡英、安斎和也、近田裕美、峯徹哉
    • Organizer
      第15回日本再生医療学会
    • Place of Presentation
      大阪国際会議場・大阪
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト多能性幹細胞由来肝前駆細胞の誘導と性状解析2015

    • Author(s)
      紙谷 聡英、鶴谷 康太、近田 裕美
    • Organizer
      第22回肝細胞研究会
    • Place of Presentation
      米子コンベンションセンター・鳥取
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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