Elucidation of hepatitis virus infection / replication mechanism using genome-modified human pluripotent stem cells
| Project/Area Number |
26293178
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAKINUMA Sei 東京医科歯科大学, 医学部, 准教授 (30372444)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | ヒトiPS細胞 / 肝炎ウイルス / 肝再生 / 肝前駆細胞 / 再生医学 / ウイルス / 幹細胞 / ウィルス |
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| Outline of Final Research Achievements |
Chronic infection of hepatitis B virus (HBV, HCV) is a major cause of cirrhosis, liver cancer etc in Japan. However, it was difficult to analyze because of the species specificity of its infectivity. In this study, for the purpose of deriving hepatocytes from human iPS cells and constructing an HBV infection analysis system, 1) preparation of highly expressed human hepatocytes of HBV receptors, 2) genes responsible for high function of hepatocytes Screening, etc. were conducted. As a result, it has been found that HBV is efficiently infected / amplified in human iPS cell-derived hepatocytes overexpressing Ntcp, and that KLF 15 is important for enhancing hepatocyte function.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Identification of a novel alpha-fetoprotein-expressing cell population induced by Jagged1/Notch2 signal in murine fibrotic liver.2017
Author(s)
Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Minako Sueoka, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
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Journal Title
Hepatology Communications
Volume: 印刷中
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Human induced pluripotent stem cell-derived hepatic cell line as a new model for host interaction with hepatitis B virus.2016
Author(s)
Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Iwamoto M, Watashi K, Wakita T, Sugiyama M, Mizokami M, Shimotohno K, Watanabe M.
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Journal Title
Sci. Rep
Volume: ６
Pages: 29358-29358
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Liver maturation deficiency in p57(Kip2)-/- mice occurs in a hepatocytic p57(Kip2) expression-independent manner2015
Author(s)
agida A, Chikada H, Ito K, Umino A, Kato-Itoh M, Yamazaki Y, Sato H, Kobayashi T, Yamaguchi T, Nakayama KI, Nakauchi H, Kamiya A.
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Journal Title
Dev. Biol.
Volume: 407(2)
Pages: 331-343
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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