Therapeutic angiogenesis using autologous adipose-derived regenerative cells
| Project/Area Number |
26293184
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
MUROHARA TOYOAKI 名古屋大学, 医学(系)研究科(研究院), 教授 (90299503)
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| Co-Investigator(Kenkyū-buntansha) |
新谷 理 名古屋大学, 医学(系)研究科(研究院), 寄附講座准教授 (20309777)
柴田 玲 名古屋大学, 医学(系)研究科(研究院), 寄附講座准教授 (70343689)
近藤 和久 名古屋大学, 医学(系)研究科(研究院), 助教 (90644659)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 再生医療 / 血管再生療法 / 幹細胞 / 四肢虚血 / 脂肪組織由来幹細胞 / 血管内皮細胞 / 皮下脂肪組織 / サイトカイン / 血管再生 / 皮下脂肪 / 間葉系幹細胞 / 虚血 / 細胞治療 / 重症四肢虚血 / 血管新生 / 脂肪組織由来再生細胞 / 心筋再生 / 細胞シート / 組織工学 / 心機能 / 虚血性心疾患 / 末梢動脈閉塞症 / 皮下脂肪由来間葉系前駆細胞 / 細胞シート工学 |
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| Outline of Final Research Achievements |
Our final goal of this research series is to establish the clinical therapeutic angiogenesis in patients with critical limb ischemia manifestating rest pain and/or ischemic skin ulcer due to atherosclerotic disease, Burger's disease and connective tissue diseases such as scleroderma (PSS). To this end, we have established mouse, rat and rabbit model of hind limb ischemia, and we examined the effects of the implantation of autologous adipose-tissue derived regenerative cells (ADRCs), one of the mesenchymal stem cell class, on limb blood perfusion and angiogenesis in these animal models. We also tested the effects of combination of several drugs and ADRCs, H2S on angiogenesis in hind limb ischemia model. We have already performed clinical cases using ADRC injection to patients with critical limb ischemia, and examined the possible biomarkers that can predict the salutary efficacy of this procedure. For example, we are focusing on the role of VEGF-A165b and circulating CD34+ cells.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Cathepsin S Activity Controls Injury-Related Vascular Repair in Mice via the TLR2-Mediated p38MAPK and PI3K-Akt/p-HDAC6 Signaling Pathway.2016
Author(s)
Wu H, Cheng XW, Hu L, Takeshita K, Chen H, Du Q, Li X, Zhu E, Huang Z, Yisireyili M, Zhao G, Piao L, Inoue A, Jiang H, Lei Y, Zhang X, Liu S, Dai Q, Kuzuya M, Shi GP, and Murohara T.
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Journal Title
Arterioscler. Thromb. Vasc. Biol.
Volume: 36(8)
Pages: 1549-1557
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Sodium Sulfide Attenuates Ischemic-Induced Heart Failure by Enhancing Proteasomal Function in an Nrf2-Dependent Manner.2016
Author(s)
Shimizu Y, Nicholson CK, Lambert JP, Barr LA, Kuek N, Herszenhaut D, Tan L, Murohara T, Hansen JM, Husain A, Naqvi N, Calvert JW.
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Journal Title
Circ. Heart Fail.
Volume: 9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Multilayered adipose-derived regenerative cell sheets created by a novel magnetite tissue engineering method for myocardial infarction.2014
Author(s)
Ishii M, Shibata R, Shimizu Y, Yamamoto T, Kondo K, Inoue Y, Ouchi N, Tanigawa T, Kanemura N, Ito A, Honda H, Murohara T.
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Journal Title
International Journal of Cardiology
Volume: 175
Pages: 545-53
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Neuron-derived neurotrophic factor functions as a novel modulator that enhances endothelial cell function and revascularization processes.2014
Author(s)
Ohashi K, Enomoto T, Joki Y, Shibata R, Ogura Y, Kataoka Y, Shimizu Y, Kambara T, Uemura Y, Yuasa D, Matsuo K, Hayakawa S, Hiramatsu-Ito M, Murohara T, Ouchi N.
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Journal Title
Journal of Biological Chemistry
Volume: 289
Pages: 14132-14144
DOI
Related Report
Peer Reviewed
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[Journal Article] Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.2014
Author(s)
Jiang H, Cheng XW, Shi GP, Hu L, Inoue A, Yamamura Y, Wu H, Takeshita K, Li X, Huang Z, Song H, Asai M, Hao CN, Unno K, Koike T, Oshida Y, Okumura K, Murohara T, Kuzuya M
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Journal Title
Nature Communication
Volume: 5
Pages: 3838-3838
DOI
Related Report
Peer Reviewed
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[Journal Article] An anti-angiogenic isoform of VEGF-A contributes to impaired vascularization in peripheral artery disease.2014
Author(s)
Kikuchi R, Nakamura K, MacLauchlan S, Ngo DT, Shimizu I, Fuster JJ, Katanasaka Y, Yoshida S, Qiu Y, Yamaguchi TP, Matsushita T, Murohara T, Gokce N, Bates DO, Hamburg NM, Walsh K
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Journal Title
Nat. Med.
Volume: 20(12)
Pages: 1464-1471
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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