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Development of chondrocyte differentiation method from human iPS cells

Research Project

Project/Area Number 26293330
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

SAITO Taku  東京大学, 医学部附属病院, 講師 (30456107)

Co-Investigator(Kenkyū-buntansha) 門野 夕峰  東京大学, 医学部附属病院, 准教授 (70401065)
矢野 文子  東京大学, 医学部附属病院, 助教 (80529040)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords整形外科学 / 変形性関節症 / 再生医療学 / 軟骨再生 / 再生医学 / iPS細胞 / 軟骨細胞 / 再生医療
Outline of Final Research Achievements

iPS cell is attractive cell source for regenerative medicine. The present study has established a novel chondrocyte induction protocol from human iPS cells by only two chemical compounds within one week. We did not find any undifferentiated cells after one week culture by the protocol, indicating the markedly high efficiency. When we implanted the differentiated cells into mouse knee joints, hyaline cartilage formation was observed.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(36 results)

All 2017 2016 2015 2014 Other

All Journal Article (19 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 19 results,  Open Access: 14 results,  Acknowledgement Compliant: 19 results) Presentation (15 results) (of which Int'l Joint Research: 2 results,  Invited: 11 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Different regulation of limb development by p63 transcript variants2017

    • Author(s)
      Kawata M, Taniguchi Y, Mori D, Yano F, Ohba S, Chung UI, Shimogori T, Mills AA, Tanaka S, Saito T
    • Journal Title

      PLOS ONE

      Volume: 12

    • DOI

      10.1371/journal.pone.0174122

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments.2017

    • Author(s)
      Sumiyo Watanabe , Toru Ogasawara, Yoshifuru Tamura, Taku Saito, Toshiyuki Ikeda, Nobuchika Suzuki, Tatsuo Shimosawa, Shigeru Shibata, Ung-il Chung, Masaomi Nangaku, Shunya Uchida
    • Journal Title

      PLOS One

      Volume: 12 Pages: 1-14

    • DOI

      10.1371/journal.pone.0168638

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regulation of Chondrocyte Survival in Mouse Articular Cartilage by p63.2017

    • Author(s)
      Taniguchi Y, Kawata M, Chang SH, Mori D, Okada K, Kobayashi H, Sugita S, Hosaka Y, Inui H, Taketomi S, Yano F, Ikeda T, Akiyama H, Mills AA, Chung UI, Tanaka S, Kawaguchi H, and Saito T*
    • Journal Title

      Arthritis & Rheumatology

      Volume: 69 Pages: 598-609

    • DOI

      10.1002/art.39976

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes.2016

    • Author(s)
      Kobayashi H, Chang S, Mori D, Itoh S, Hirata M, Hosaka Y, Taniguchi Y, Okada K, Mori Y, Yano F, Chung U, Akiyama H, Kawaguchi H, Tanaka S, Saito T.
    • Journal Title

      Nat Commun.

      Volume: 7 Pages: 13336-13336

    • DOI

      10.1038/ncomms13336

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment.2016

    • Author(s)
      H. Aini, K. Itaka, A. Fujisawa, H. Uchida, S. Uchida, S. Fukushima, K. Kataoka, T. Saito, U. -I. Chung, S. Ohba
    • Journal Title

      Sci. Rep.

      Volume: 6 Pages: 18743-18743

    • DOI

      10.1038/srep18743

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparison of mouse and human ankles and establishment of mouse ankle osteoarthritis models by surgically-induced instability2016

    • Author(s)
      Chang SH, Yasui T, Taketomi S, Matsumoto T, Kim-Kaneyama JR, Omiya T, Hosaka Y, Inui H, Omata Y, Yamagami R, Mori D, Yano F, Chung U, Tanaka S, Saito T.
    • Journal Title

      Osteoarthritis Cartilage.

      Volume: 24 Pages: 688-697

    • DOI

      10.1016/j.joca.2015.11.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] <b>Hyaline cartilage formation and tumorigenesis of implanted tissues derived from human induced pluripotent stem </b><b>cells </b>2015

    • Author(s)
      Saito T, Yano F, Mori D, Kawata M, Hoshi K, Takato T, Masaki H, Otsu M, Eto K, Nakauchi H, Chung UI, Tanaka S
    • Journal Title

      Biomed. Res.

      Volume: 36 Issue: 3 Pages: 179-186

    • DOI

      10.2220/biomedres.36.179

    • NAID

      130005083858

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factor Hes1 modulates osteoarthritis development in cooperation with calcium/calmodulin-dependent protein kinase 2.2015

    • Author(s)
      Sugita S, Taketomi S, Saito T, et al.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 112 Pages: 3080-3085

    • DOI

      10.1073/pnas.1419699112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Individual and combining effects of anti-RANKL monoclonal antibody and teriparatide in ovariectomized mice.2015

    • Author(s)
      Tokuyama N, Hirose J, Omata Y, Yasui T, Izawa N, Matsumoto T, Masuda H, Ohmiya T,Yasuda H, Saito T, Kadono Y, Tanaka S
    • Journal Title

      Bone Reports.

      Volume: 2 Pages: 1-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] <b>Regulation of mouse chondrocyte differentiation by CCAAT/enhancer-binding pr</b><b>oteins </b>2015

    • Author(s)
      Okuma T, Hirata M, Yano F, Mori D, Kawaguchi H, Chung UI, Tanaka S, Saito T
    • Journal Title

      Biomed. Res.

      Volume: 36 Issue: 1 Pages: 21-29

    • DOI

      10.2220/biomedres.36.21

    • NAID

      130004903876

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome-wide comprehensive analysis reveals critical cooperation between Smad and c-Fos in RANKL-induced osteoclastogenesis2015

    • Author(s)
      Omata Y, Yasui T, Hirose J, Izawa N, Imai Y, Matsumoto T, Masuda H, Tokuyama N, Nakamura S, Tsutsumi S, Yasuda H, Okamoto K, Takayanagi H, Hikita A, Imamura T, Matsuo K, Saito T, Kadono Y, Aburatani H, Tanaka S
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: 30 Pages: 869-77

    • DOI

      10.1002/jbmr.2418

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of SCAN domain zinc-finger gene ZNF449 as a novel factor of chondrogenesis.2014

    • Author(s)
      Okada K, Saito T, et al.
    • Journal Title

      PLoS One

      Volume: 9

    • DOI

      10.1371/journal.pone.0115169

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] SOX10 transactivates S100B to suppress Schwann cell proliferation and to promote myelination.2014

    • Author(s)
      Fujiwara S, Hoshikawa S, Ueno T, Hirata M, Saito T, Ikeda T, Kawaguchi H, Nakamura K, Tanaka S, Ogata T
    • Journal Title

      PLoS One

      Volume: 9

    • DOI

      10.1371/journal.pone.0115400

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Determination of differential gene expression profiles in superficial and deeper zones of mature rat articular cartilage using RNA sequencing of laser microdissected tissue specimens.2014

    • Author(s)
      Mori Y, Chung UI, Tanaka S, Saito T
    • Journal Title

      Biomed Res

      Volume: 35 Pages: 263-70

    • NAID

      130004678582

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] S100A1 and S100B are dispensable for endochondral ossification during skeletal development2014

    • Author(s)
      Mori Y, Mori D, Chung UI, Tanaka S, Heierhorst J, Buchou T, Baudier J, Kawaguchi H, Saito T
    • Journal Title

      Biomed Res

      Volume: 35 Pages: 243-50

    • NAID

      130004678580

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Disease-modifying effects of TD-198946 on progressed osteoarthritis in a mouse model.2014

    • Author(s)
      Yano F, Saito T, et al.
    • Journal Title

      Ann Rheum Dis

      Volume: 73 Pages: 2062-2064

    • DOI

      10.1136/annrheumdis-2014-205672

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions.2014

    • Author(s)
      Kanke K, Masaki H, Saito T, Komiyama Y, Hojo H, Nakauchi H, Lichtler AC, Takato T, Chung UI, Ohba S
    • Journal Title

      Stem Cell Reports

      Volume: 6 Pages: 751-60

    • DOI

      10.1016/j.stemcr.2014.04.016

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of fibroblast growth factor-18 as a molecule to protect adult articular cartilage by gene expression profiling.2014

    • Author(s)
      Mori Y, Saito T, Chang SH, Kobayashi H, Ladel CH, Guehring H, Chung UI, Kawaguchi H.
    • Journal Title

      J Biol Chem.

      Volume: 289 Pages: 10192-200

    • DOI

      10.1074/jbc.m113.524090

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Positive feedback between NF-κB and TNF-α promotes leukemia-initiating cell capacity.2014

    • Author(s)
      Kagoya Y, Yoshimi A, Kataoka K, Nakagawa M, Kumano K, Arai S, Kobayashi H, Saito T, Iwakura Y, Kurokawa M.
    • Journal Title

      Journal of Clinical Investigation

      Volume: 124 Pages: 528-42

    • DOI

      10.1172/jci68101

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 過剰な力学的ストレス負荷による関節軟骨変性の分子メカニズム2016

    • Author(s)
      齋藤琢
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 変性性関節症の制御メカニズム2016

    • Author(s)
      齋藤琢
    • Organizer
      Orthopedic Research Club
    • Place of Presentation
      木更津
    • Year and Date
      2016-11-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 過剰な力学的ストレスによる関節軟骨異化のメカニズム2016

    • Author(s)
      齋藤琢
    • Organizer
      第10回骨軟骨フロンティア
    • Place of Presentation
      東京
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Regulation of Articular Cartilage Homeostasis and Osteoarthritis Development by the NF-kappaB signaling.2016

    • Author(s)
      Taku Saito
    • Organizer
      2016 Cold Spring Harbor Asia Conference on Bone & Cartilage
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Notch・Hes1による軟骨細胞の制御機構2016

    • Author(s)
      齋藤琢
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 過剰な力学的ストレスによる関節軟骨異化のメカニズム2016

    • Author(s)
      齋藤琢
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Perspective of disease-modifying osteoarthritis drugs2016

    • Author(s)
      Taku Saito
    • Organizer
      第3回アジア太平洋骨代謝学会議
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Establishment of Surgical Destabilization Model of Mouse Ankle Osteoarthritis2015

    • Author(s)
      Chang SH, Saito T, et al.
    • Organizer
      Annual meeting of the Orthopaedic Research Society.
    • Place of Presentation
      Las vegas, USA
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 軟骨内骨化研究から生まれる実用的研究2014

    • Author(s)
      齋藤琢
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-10-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 変形性関節症への応用を目指した軟骨再生研究2014

    • Author(s)
      齋藤琢
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-10-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] HIF-1α IS ESSENTIAL FOR ARTICULAR CARTILAGE HOMEOSTASIS THROUGH INDUCTION OF ANABOLIC FACTORS AND SUPPRESSION OF CATABOLIC FACTORS.2014

    • Author(s)
      Okada K, Saito T, et al.
    • Organizer
      Annual meeting of the American Society for bone and mineral research.
    • Place of Presentation
      Houston USA
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of Grem1 as a catabolic factor induced by mechanical stress loading in articular chondrocyte.2014

    • Author(s)
      Chang SH, Saito T, et al.
    • Organizer
      Annual meeting of the American Society for bone and mineral research.
    • Place of Presentation
      Houston USA
    • Year and Date
      2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Expression and function of CCAAT/enhancer-binding protein family in chondrocytes.2014

    • Author(s)
      Okuma T, Saito T, et al.
    • Organizer
      Annual meeting of the American Society for bone and mineral research.
    • Place of Presentation
      Houston USA
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 変形性関節症の制御メカニズムの解明2014

    • Author(s)
      齋藤琢
    • Organizer
      第32回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Research on pathophysiology and therapeutic target of osteoarthritis.2014

    • Author(s)
      Taku Saito
    • Organizer
      16th Asia Pacific League of Associations for Rheumatology Congress (APLAR)
    • Place of Presentation
      Cebu City, Philippines
    • Year and Date
      2014-04-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] 東京大学医学部整形外科

    • URL

      http://www.u-tokyo-ortho.jp/

    • Related Report
      2016 Annual Research Report 2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 軟骨細胞の製造方法2016

    • Inventor(s)
      齋藤琢、ほか
    • Industrial Property Rights Holder
      齋藤琢、ほか
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-163872
    • Filing Date
      2016-08-24
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2019-12-27  

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