Development of biomarkers and novel therapeutic targets for malignant bone and soft tissue tumors using the methods of global expression analysis

• Project/Area Number: 26293342
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: National Cancer Center Japan
• Principal Investigator: KAWAI AKIRA 国立研究開発法人国立がん研究センター, 骨軟部腫瘍・リハビリテーション科, 科長 (90252965)
• Co-Investigator(Kenkyū-buntansha): 近藤 格 国立研究開発法人国立がん研究センター, 研究所, 分野長 (30284061) ; 落谷 孝広 国立研究開発法人国立がん研究センター, 研究所, 主任分野長 (60192530) ; 市川 仁 国立研究開発法人国立がん研究センター, 先端医療開発センター, ユニット長 (30201924) ; 吉田 朗彦 国立研究開発法人国立がん研究センター, 中央病院, 医員 (80574780) ; 細野 亜古 国立研究開発法人国立がん研究センター, 東病院, 医長 (00602947) ; 小倉 浩一 国立研究開発法人国立がん研究センター, 中央病院, 特任研究員 (20583115)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 骨軟部腫瘍 / 肉腫 / 骨肉腫 / 粘液線維肉腫 / 粘液型脂肪肉腫 / 遺伝子 / microRNA / タンパク質 / ゲノム解析 / エピゲノム解析 / プロテオーム解析 / PDXモデル / 医療・福祉 / 癌 / バイオマーカー / 軟骨肉腫 / ゼノグラフト / 全エクソン解析 / 全トランスクリプトーム解析 / 悪性骨軟部腫瘍 / 胞巣状軟部肉腫 / 全ゲノム解析

Outline of Final Research Achievements

In the current study, we performed whole exome and transcriptome sequences of chondrosarcoma, myxofibrosarcoma and giant cell tumor of bone. Myxofibrosarcoma was divided into 3 groups depending on the epigenome characteristics.　Invasiveness and potential of pulmonary metastasis were regulated by microRNA133a in osteosarcoma and by microRNA135b in myxoid liposarcoma. Immunohistochemistries for TFE3, FGF23 and H3K27me3, and FISH for WT1 and USP6 were useful for pathological diagnosis of bone and soft tissue sarcomas.

Research Products

• [Journal Article] Cross-cultural adaptation and validation of the Japanese version of the Toronto Extremity Salvage Score (TESS) for patients with malignant musculoskeletal tumors in the upper extremities (2017)
  • Author(s): Akiyama T, Uehara K, Ogura K, Shinoda Y, Iwata S, Saita K, Tanzawa Y, Nakatani F, Yonemoto T, Kawano H, Davis AM, Kawai A.
  • Journal Title: Journal of Orthopaedic Science Volume: 22(1) Pages: 127-132
  • DOI: 10.1016/j.jos.2016.09.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phase 2 study of eribulin in patients with previously treated advanced or metastatic soft tissue sarcoma. (2017)
  • Author(s): Kawai A, Araki N, Naito Y, Ozaki T, Sugiura H, Yazawa Y, Morioka H, Matsumine A, Saito K, Asami S, Isu K.
  • Journal Title: Jpn J Clin Oncol Volume: 47(2) Pages: 137-144
  • DOI: 10.1093/jjco/hyw175
  • Peer Reviewed / Open Access
• [Journal Article] Proteomic study of hepatocellular carcinoma using a novel modified aptamer-based array (SOMAscan) platform (2017)
  • Author(s): Qiao Z, Pan X, Parlayan C, Ojima H, Kondo T.
  • Journal Title: Biochim Biophys Acta Volume: 4 Pages: 434-443
  • DOI: 10.1016/j.bbapap.2016.09.011
  • Peer Reviewed / Open Access
• [Journal Article] Frequent amplification of receptor tyrosine kinase genes in welldifferentiated/ dedifferentiated liposarcoma. (2017)
  • Author(s): Asano N, Yoshida A, Mitani S, Kobayashi E, Shiotani B, Komiyama M, Fujimoto H, Chuman H, Morioka H, Matsumoto M, Nakamura M, Kubo T, Kato M, Kohno T, Kawai A, Kondo T, Ichikawa H.
  • Journal Title: Oncotarget. Volume: 8 Pages: 12941-12952
  • DOI: 10.18632/oncotarget.14652
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemistry for trimethylated H3K27 in the diagnosis of malignant peripheral nerve sheath tumours. (2017)
  • Author(s): Asano N, Yoshida A, Ichikawa H, Mori T, Nakamura M, Kawai A, Hiraoka N.
  • Journal Title: Histopathology Volume: 70 Pages: 385-393
  • DOI: 10.1111/his.13072
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Highly recurrent H3F3A mutations with additional epigenetic regulator alterations in giant cell tumor of bone. (2017)
  • Author(s): Ogura K, Hosoda F, Nakamura H, Hama N, Totoki Y, Yoshida A, Ohashi S, Rokutan H, Takai E, Yachida S, Kawai A, Tanaka S, Shibata T.
  • Journal Title: Genes Chromosomes Cancer. Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance. (2016)
  • Author(s): Kim Y, Kobayashi E, Kubota D, Suehara Y, Mukaihara K, Akaike K, Ito A, Kaneko K, Chuman H, Kawai A, Kitano S.
  • Journal Title: Oncotarget. Volume: 7(43) Pages: 70832-70844
  • DOI: 10.18632/oncotarget.12225
  • Peer Reviewed / Open Access
• [Journal Article] Results of sub-analysis of a phase 2 study on trabectedin treatment for extraskeletal myxoid chondrosarcoma and mesenchymal chondrosarcoma (2016)
  • Author(s): Morioka H, Takahashi S, Araki N, Sugiura H, Ueda T, Takahashi M, Yonemoto T, Hiraga H, Hiruma T, Kunisada T, Matsumine A, Susa M, Nakayama R, Nishimoto K, Kikuta K, Horiuchi K, Kawai A
  • Journal Title: BMC Cancer. Volume: 16 Pages: 479-479
  • DOI: 10.1186/s12885-016-2511-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reliability and Validity of a Japanese-language and Culturally Adapted Version of the Musculoskeletal Tumor Society Scoring System for the Lower Extremity. (2016)
  • Author(s): Iwata S, Uehara K, Ogura K, Akiyama T, Shinoda Y, Yonemoto T, Kawai A
  • Journal Title: Clin Orthop Relat Res. Volume: 474(9) Pages: 2044-2052
  • DOI: 10.1007/s11999-016-4880-6
  • Peer Reviewed / Open Access
• [Journal Article] The clinical outcome of pazopanib treatment in Japanese patients with relapsed soft tissue sarcoma: A Japan Musculoskeletal Oncology Group (JMOG) study. (2016)
  • Author(s): Nakamura T, Matsumine A, Kawai A, Araki N, Goto T, Yonemoto T, Sugiura H, Nishida Y, Hiraga H, Honoki K, Yasuda T, Boku S, Sudo A, Ueda T.
  • Journal Title: Cancer. Volume: 122(9) Pages: 1408-1416
  • DOI: 10.1002/cncr.29961
  • Peer Reviewed / Open Access
• [Journal Article] A randomized, double-blind, placebo-controlled, Phase III study of pazopanib in patients with soft tissue sarcoma: results from the Japanese subgroup. (2016)
  • Author(s): Kawai A, Araki N, Hiraga H, Sugiura H, Matsumine A, Ozaki T, Ueda T, Ishii T, Esaki T, Machida M, Fukasawa N.
  • Journal Title: Jpn J Clin Oncol. Volume: 46(3) Pages: 248-253
  • DOI: 10.1093/jjco/hyv184
  • Peer Reviewed / Open Access
• [Journal Article] Retrospective inter- and intra-patient evaluation of trabectedin after best supportive care for patients with advanced translocation-related sarcoma after failure of standard chemotherapy. (2016)
  • Author(s): Araki N, Takahashi S, Sugiura H, Ueda T, Yonemoto T, Takahashi M, Morioka H, Hiraga H, Hiruma T, Kunisada T, Matsumine A, Kawai A.
  • Journal Title: Eur J Cancer. Volume: 56 Pages: 122-130
  • DOI: 10.1016/j.ejca.2015.12.014
  • Peer Reviewed / Open Access
• [Journal Article] miR-125b and miR-100 Are Predictive Biomarkers of Response to Induction Chemotherapy in Osteosarcoma (2016)
  • Author(s): Kubota D, Kosaka N, Fujiwara T, Yoshida A, Arai Y, Qiao Z, Takeshita F, Ochiya T, Kawai A, Kondo T.
  • Journal Title: Sarcoma Volume: 2016 Pages: 1390571-1390571
  • DOI: 10.1155/2016/1390571
  • Peer Reviewed / Open Access
• [Journal Article] Synovial sarcoma cell lines showed reduced DNA repair activity and sensitivity to a PARP inhibitor (2016)
  • Author(s): Yamasaki H, Miyamoto M, Yamamoto Y, Kondo T, Watanabe T, Ohta T.
  • Journal Title: Genes Cells Volume: 21(8) Pages: 852-560
  • DOI: 10.1111/gtc.12387
  • Peer Reviewed / Open Access
• [Journal Article] Proteogenomics for the Study of Gastrointestinal Stromal Tumors (2016)
  • Author(s): Kondo T.
  • Journal Title: Adv Exp Med Biol Volume: 926 Pages: 139-151
  • DOI: 10.1007/978-3-319-42316-6_9
  • Peer Reviewed / Open Access
• [Journal Article] Novel p53 target gene FUCA1 encodes a fucosidase and regulates growth and survival of cancer cells (2016)
  • Author(s): Ezawa I, Sawai Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Kobayashi Y, Tashiro F, Namiki H, Kondo T, Semba K, Aburatani H, Taya Y, Nakagama H, Ohki R.
  • Journal Title: Cancer Sci Volume: 印刷中 Pages: 434-435
  • DOI: 10.1111/cas.12933
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] PAI-1, a target gene of miR-143, regulates invasion and metastasis by up-regulating MMP-13 expression of human osteosarcoma (2016)
  • Author(s): Hirahata M, Osaki M, Kanda Y, Sugimoto Y, Yoshioka Y, Kosaka N, Takeshita F, Fujiwara T, Kawai A, Ito H, Ochiya T, Okada F.
  • Journal Title: Cancer Med. Volume: - Pages: 892-902
  • DOI: 10.1002/cam4.651
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] miR-135b, a key regulator of malignancy, is linked to poor prognosis in human myxoid liposarcoma. (2016)
  • Author(s): Nezu Y, Hagiwara K, Yamamoto Y, Fujiwara T, Matsuo K, Yoshida A, Kawai A, Saito T, Ochiya T.
  • Journal Title: Oncogene. Volume: 35 Pages: 6177-6188
  • DOI: 10.1038/onc.2016.157
  • Peer Reviewed / Open Access
• [Journal Article] Follicular dendritic cell sarcoma treated with a variety of chemotherapy. (2016)
  • Author(s): Sasaki M, Izumi H, Yokoyama T, Kojima M, Hosono A.
  • Journal Title: Hematol Oncol. Volume: 8 Pages: 725-728
  • DOI: 10.1002/ajh.20852
  • Peer Reviewed / Open Access
• [Journal Article] Trabectedin monotherapy after standard chemotherapy versus best supportive care in patients with advanced, translocation-related sarcoma: a randomised, open-label, phase 2 study. (2016)
  • Author(s): Kawai A, Araki N, Sugiura H, Ueda T, Yonemoto T, Takahashi M, Morioka H, Hiraga H, Hiruma T, Kunisada T, Matsumine A, Tanase T, Hasegawa T, Takahashi S.
  • Journal Title: Lancet Oncol. Volume: 16 Pages: 406-16
  • DOI: 10.1016/s1470-2045(15)70098-7
  • Peer Reviewed / Open Access
• [Journal Article] CIC-rearranged Sarcomas: A Study of 20 Cases and Comparisons With Ewing Sarcomas. (2016)
  • Author(s): Yoshida A, Goto K, Kodaira M, Kobayashi E, Kawamoto H, Mori T, Yoshimoto S, Endo O, Kodama N, Kushima R, Hiraoka N, Motoi T, Kawai A.
  • Journal Title: Am J Surg Pathol. Volume: 40(3) Pages: 313-23
  • Peer Reviewed / Open Access
• [Journal Article] The diagnostic utility of reduced immunohistochemical expression of SMARCB1 in synovial sarcomas: a validation study. (2016)
  • Author(s): Ito J, Asano N, Kawai A, Yoshida A.
  • Journal Title: Hum Pathol. Volume: 47(1) Pages: 32-7
  • DOI: 10.1016/j.humpath.2015.09.010
  • Peer Reviewed / Open Access
• [Journal Article] Current status of publicly available sarcoma cell lines for use in proteomic studies. (2016)
  • Author(s): Pan X, Yoshida A, Kawai A, Kondo T.
  • Journal Title: Expert Rev Proteomics. Volume: 13(2) Pages: 227-40
  • DOI: 10.1586/14789450.2016.1132166
  • Peer Reviewed / Open Access
• [Journal Article] Differential SALL4 immunoexpression in malignant rhabdoid tumours and epithelioid sarcomas. (2015)
  • Author(s): Yoshida A, Asano N, Kawai A, Kawamoto H, Nakazawa A, Kishimoto H, Kushima R.
  • Journal Title: Histopathology. Volume: 66(2) Pages: 252-61
  • DOI: 10.1111/his.12460
  • Peer Reviewed / Open Access
• [Journal Article] Second primary osteosarcomas in patients with retinoblastoma. (2015)
  • Author(s): Fujiwara T, Fujiwara M, Numoto , Ogura K, Yoshida A, Yonemoto T, Suzuki S, Kawai A
  • Journal Title: Jpn J Clin Oncol. Volume: 45(12) Pages: 1139-45
  • DOI: 10.1093/jjco/hyv140
  • Peer Reviewed / Open Access
• [Journal Article] Myoepithelioma-like Tumors of the Vulvar Region: A Distinctive Group of SMARCB1-deficient Neoplasms. (2015)
  • Author(s): Yoshida A, Yoshida H, Yoshida M, Mori T, Kobayashi E, Tanzawa Y, Yasugi T, Kawana K, Ishikawa M, Sugiura H, Maeda D, Fukayama M, Kawai A, Hiraoka N, Motoi T.
  • Journal Title: Am J Surg Pathol. Volume: 39(8) Pages: 1102-13
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic significance of promyelocytic leukemia expression in gastrointestinal stromal tumor; integrated proteomic and transcriptomic analysis. (2015)
  • Author(s): 1.Ichikawa H, Yoshida A, Kanda T, Kosugi S, Ishikawa T, Nahyu T, Taguchi T, Sakumoto M, Katai H, Kawai A, Wakai T, Kondo T
  • Journal Title: Cancer Sci. Volume: 106(1) Pages: 115-24
  • DOI: 10.1111/cas.12565
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interactome analysis reveals molecular mechanisms underlying the association between selenium binding protein 1 expression and the malignant features of tumor cells (2015)
  • Author(s): Tajima T, Kito F, Ohta T, Shiozawa K, Kawai A, Kondo T.
  • Journal Title: J. Electrophor Volume: 59 Issue: 1 Pages: 1-1
  • DOI: 10.2198/jelectroph.59.1
  • NAID: 130005130417
  • ISSN: 1349-9394, 1349-9408
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Global protein-expression profiling for reclassification of malignant fibrous histiocytoma (2015)
  • Author(s): Kikuta K, Morioka H, Kawai A, Kondo
  • Journal Title: Biochim Biophys Acta. Volume: 1854(6) Pages: 696-701
  • DOI: 10.1016/j.bbapap.2014.08.012
  • Peer Reviewed / Open Access
• [Journal Article] Current advances in esophageal cancer proteomics. (2015)
  • Author(s): Uemura N, Kondo T.
  • Journal Title: Biochim Biophys Acta. Volume: 1854(6) Pages: 687-95
  • DOI: 10.1016/j.bbapap.2014.09.011
  • Peer Reviewed
• [Journal Article] Clinical relecance and therapeutic significance of microRNA-133a expression profiles and functions in malignant osteosarcoma-initiating cells (2014)
  • Author(s): Fujiwara T, Katsuda T, Hagiwara K, Kosaka N, Yoshioka Y, Takahashi RU, Takeshita F, Kubota D, Kondo T, Ichikawa H, Yoshida A, Kobayashi E, Kawai A, Ozaki T, Ochiya T.
  • Journal Title: Stem Cells Volume: 32 Pages: 959-73
  • DOI: 10.1002/stem.1618
  • Peer Reviewed / Open Access
• [Journal Article] Phase I and pharmacokinetic study of trabectedin, a DNA minor groove binder, administered as a 24-h continuous infusion in Japanese patients with soft tissue sarcoma. (2014)
  • Author(s): Ueda T, Kakunaga S, Sugiura , Kawai A, et al.
  • Journal Title: Invest New Drugs Volume: 32(4) Pages: 691-9
  • DOI: 10.1007/s10637-014-0094-5
  • Peer Reviewed
• [Journal Article] STAT6 immunohistochemistry is helpful in the diagnosis of solitary fibrous tumors. (2014)
  • Author(s): Yoshida A, Narita Y, Kawai A, Asamura H, et al.
  • Journal Title: Am J Surg Pathol Volume: 38(4) Pages: 552-9
  • DOI: 10.1097/pas.0000000000000137
  • Peer Reviewed
• [Journal Article] Proteomics identified overexpression of SET oncogene product and possible therapeutic utility of protein phosphatase 2A in alveolar soft part sarcoma. (2014)
  • Author(s): Kubota D, Yoshida A, Kawai A, Kondo T.
  • Journal Title: J Proteome Res Volume: 13 Pages: 2250-2261
  • DOI: 10.1021/pr400929h
  • Peer Reviewed
• [Journal Article] International expert opinion on patient-tailored management of soft tissue sarcomas. (2014)
  • Author(s): Blay JY, Kawai A,et al.
  • Journal Title: Eur J Cancer Volume: 50 Pages: 679-689
  • DOI: 10.1016/j.ejca.2013.11.011
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA Expression and Functional Profiles of Osteosarcoma. (2014)
  • Author(s): Kobayashi E, Satow R, Ono M, Masuda M, Honda K, Sakuma T, Kawai A, Morioka H, Toyama Y, Yamada T.
  • Journal Title: Oncology. Volume: 86(2): Pages: 94-103
  • DOI: 10.1159/000357408
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic factors in elderly osteosarcoma patients: a multi-institutional retrospective study of 86 cases. (2014)
  • Author(s): Iwata S, Ishii T, Kawai A, Hiruma T, Yonemoto T, Kamoda H, Asano N, Takeyama M.
  • Journal Title: Ann Surg Oncol Volume: 21 Pages: 263-268
  • DOI: 10.1245/s10434-013-3210-4
  • Peer Reviewed / Open Access
• [Journal Article] Reversible hair depigmentation in a Japanese female treated with pazopanib. (2014)
  • Author(s): Kobayashi E, Koyama T, Kobayashi K, Setsu N, Kawashima M, Kawai A.
  • Journal Title: J Dermatol Volume: 41(11) Pages: 1021-2
  • DOI: 10.1111/1346-8138.12654
  • Peer Reviewed / Open Access
• [Journal Article] Chronic expanding hematoma with a significantly high fluorodeoxyglucose uptake on １８F-fluorodeoxyglucose positron emission tomography, mimicking a malignant soft tissue tumor: a case report. (2014)
  • Author(s): Nishida Y, Kobayashi E, Kubota D, Setsu N, Ogura K, Tanzawa Y, Nakatani F, Kato Y, Chuman H, Kawai A
  • Journal Title: J Med Case Rep Volume: 8 Pages: 349-349
  • DOI: 10.1186/1752-1947-8-349
  • Peer Reviewed / Open Access
• [Journal Article] Multiple metastases from histologically benign intraarticular diffuse-type tenosynovial giant cell tumor: a case report. (2014)
  • Author(s): Asano N, Yoshida A, Kobayashi E, Yamaguchi T, Kawai A.
  • Journal Title: Hum Pathol Volume: 45(11) Pages: 2355-8
  • DOI: 10.1016/j.humpath.2014.06.025
  • Peer Reviewed / Open Access
• [Journal Article] Inconvenient truth: Cancer biomarker development by using proteomics (2014)
  • Author(s): Kondo T
  • Journal Title: Biochim Biophys Acta Volume: 1844(5) Pages: 861-5
  • DOI: 10.1016/j.bbapap.2013.07.009
  • Peer Reviewed / Open Access
• [Journal Article] Casting doubt on the traditional approach of cancer biomarker scovery through proteomics (2014)
  • Author(s): Kondo T
  • Journal Title: Expert Rev Proteomics. Volume: 11(1) Pages: 9-12
  • DOI: 10.1586/14789450.2014.871496
  • Peer Reviewed / Open Access
• [Journal Article] Global PROTOMAP profiling to search for biomarkers of earely-recurrent hepatocellular carcinoma. (2014)
  • Author(s): Taoka, Masato; Morofuji, Noriaki; Yamauchi, Yoshio; Ojima, Hidenori; Kubota, Daisuke; Terukina, Goro; Nobe, Yuko; Nakayama, Hiroshi; Takahashi, Nobuhiro; Kosuge, Tomoo; Isobe, Toshiaki; Kondo, Tadashi
  • Journal Title: J Proteome Res Volume: 13 Pages: 4847-4858
  • DOI: 10.1021/pr500262p
  • Peer Reviewed
• [Journal Article] RPN2 Gene Confers Osteosarcoma Cell Malignant Phenotypes and Determines Clinical Prognosis. (2014)
  • Author(s): Fujiwara T, Takahashi RU, Kosaka N, Nezu Y, Kawai A, Ozaki
  • Journal Title: Mol Ther Nucleic Acids. Volume: 3
  • DOI: 10.1038/mtna.2014.35
  • Peer Reviewed / Open Access
• [Journal Article] The role of microRNAs in the regulation of cancer stem cells. (2014)
  • Author(s): Takahashi RU, Miyazaki H, Ochiya T.
  • Journal Title: Front Genet. Volume: 4 Pages: 295-295
  • DOI: 10.3389/fgene.2013.00295
  • Peer Reviewed / Open Access
• [Journal Article] miRNAの発現抑制による骨肉腫を含むがん治療への応用 (2014)
  • Author(s): 藤原智洋, 尾崎敏文, 川井章, 落谷孝広
  • Journal Title: 医薬ジャーナル Volume: 50 Pages: 97-104
  • Peer Reviewed
• [Presentation] miR-135b is a key regulator of malignancy of human myxoid liposarcoma (2017)
  • Author(s): Nezu Y, Kawai A, Saito T, Ochiya T.
  • Organizer: Orthopaedic Research Society (ORS) Annual Meeting
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] Challenges and resource stratified management of soft tissue sarcoma treatment in Asia, Introduction (2016)
  • Author(s): Akira Kawai
  • Organizer: ESMO Asia 2016
  • Place of Presentation: Singapore
  • Year and Date: 2016-12-16
  • Int'l Joint Research
• [Presentation] Evolving Treatment of Sarcomas ;The role of multi-institutional and international collaboration (2016)
  • Author(s): Akira Kawai
  • Organizer: 4th Singapore Sarcoma Symposium
  • Place of Presentation: Singapore
  • Year and Date: 2016-11-05
  • Int'l Joint Research / Invited
• [Presentation] Recent Advances of Sarcoma Treatment in Japan (2016)
  • Author(s): 川井 章
  • Organizer: 第54回日本癌治療学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-20
• [Presentation] Recent Advances of Sarcoma Treatment in Japan (2016)
  • Author(s): Akira Kawai
  • Organizer: 60th Korean Orthopaedic Association
  • Place of Presentation: Incheon(Korea)
  • Year and Date: 2016-10-19
  • Invited
• [Presentation] Biomarker development by cancer proteomics, (2016)
  • Author(s): Tadashi Kondo
  • Organizer: 10th Central and Eastern European Proteome Conference
  • Place of Presentation: Budapest( Hungary)
  • Year and Date: 2016-10-11
  • Int'l Joint Research / Invited
• [Presentation] 次世代シークエンサーを用いた高分化／脱分化脂肪肉腫の治療標的探索 (2016)
  • Author(s): 浅野尚文、他
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] ゲノム解析による軟部肉腫の治療奏効性バイオマーカー探索 (2016)
  • Author(s): 三谷幸代、他
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] Proteomics approach to sarcoma research toward clinical application. (2016)
  • Author(s): Tadashi Kondo
  • Organizer: Annual Meeting of Asia Oceania Human Proteome Organization.
  • Place of Presentation: Taiwan
  • Year and Date: 2016-09-22
  • Int'l Joint Research / Invited
• [Presentation] がんのプロテオーム解析の考え方 (2016)
  • Author(s): 近藤格
  • Organizer: 第67回日本電気泳動学会総会
  • Place of Presentation: 釧路市観光国際交流センター(北海道釧路市）
  • Year and Date: 2016-08-26
• [Presentation] 患者由来ゼノグラフトパネルの整備とがん基礎研究への応用の可能性 (2016)
  • Author(s): 高橋真美，石ヶ守里加子，河野隆志，市村幸一、吉田朗彦、川井章、近藤格，今井俊夫
  • Organizer: 第31回発癌病理研究会
  • Place of Presentation: 信州松代ロイヤルホテル（長野県長野市）
  • Year and Date: 2016-08-23
• [Presentation] Systemic Treatment for Soft Tissue Sarcomas Current concepts and the latest findings (2016)
  • Author(s): 川井 章
  • Organizer: 第14回日本臨床腫瘍学会
  • Place of Presentation: 神戸国際展示場(兵庫県神戸市)
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] Sarcoma cell lines: Current status and perspectives. (2016)
  • Author(s): Xiaoqing Pan, Akihiko Yoshida, Akira Kawai, Tadashi Kondo.
  • Organizer: 第89回 日本組織培養学会年会
  • Place of Presentation: 千里ライフサイエンスセンター ( 大阪府豊中市)
  • Year and Date: 2016-05-25
• [Presentation] 骨・軟部肉腫に対する新規治療薬開発 (2016)
  • Author(s): 川井 章
  • Organizer: 第89回日本整形外科学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 肉腫のプロテオーム解析 (2016)
  • Author(s): 近藤格
  • Organizer: 第89回日本整形外科学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] Novel patient-derived xenograft and primary tissue culture cell for pre-clinical study in sarcoma. (2016)
  • Author(s): Xiaoqing Pan, Mami Takahashi, Hitoshi Ichikawa, Akihiko Yoshida, Akira Kawai, Toshio Imai, Tadashi Kondo.
  • Organizer: Annual Meeting of American Association of Cancer Research
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Round cell-specific microRNA contributes to malignancy and morphological change in myxoid liposarcoma (2016)
  • Author(s): Nezu Y, Hagiwara K, Fujiwara T, Kawai K, Matsuo K, Saito T, Ochiya T.
  • Organizer: Research Society 2016 Annual Meeting Orland
  • Place of Presentation: フロリダ（アメリカ）
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] H3K27me3免疫染色は悪性末梢神経鞘腫瘍の診断に有用である (2016)
  • Author(s): 吉田 朗彦、浅野尚文、森泰昌、川井章、平岡伸介
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター(宮城県仙台市）
• [Presentation] Treatment strategy for sarcoma in pediatric patients Past,Present and Future (2016)
  • Author(s): 細野亜古
  • Organizer: 第49回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 東京ドームホテル（東京都文京区）
• [Presentation] 難治性小児固形腫瘍に対するGPC3ペプチドワクチン療法の第I相試験 (2016)
  • Author(s): 細野亜古、金田英秀、原純一、木下義晶、孝橋賢一、真部敦、中面哲也
  • Organizer: 第58回小児血液・がん学会
  • Place of Presentation: 品川プリンスホテル（東京都港区）
• [Presentation] The role of Guidelines in improving patient care in Soft Tissue Sarcomas (2015)
  • Author(s): Akira Kawai
  • Organizer: ESMO Asia 2015
  • Place of Presentation: シンガポール
  • Year and Date: 2015-12-18
  • Int'l Joint Research / Invited
• [Presentation] ＡＹＡ世代骨軟部肉腫の外科治療 (2015)
  • Author(s): 川井 章
  • Organizer: 第57回 日本小児血液・がん学会学術集会
  • Place of Presentation: 甲府富士屋ホテル（山梨県）
  • Year and Date: 2015-11-27
  • Int'l Joint Research
• [Presentation] 人からmicroRNAを応用し、小児の骨肉腫を治す (2015)
  • Author(s): 藤原智洋, 落谷孝広
  • Organizer: 第36回動物臨床医学会記念年次大会
  • Place of Presentation: 大阪国際会議場（大阪）
  • Year and Date: 2015-11-20
  • Int'l Joint Research
• [Presentation] DENTIFICATION OF TUMOR-DERIVED CIRCULATING MICRORNA SIGNATURES IN OSTEOSARCOMA PATIENTS (2015)
  • Author(s): Fujiwara T, Uotani K, Morita T, Yoshida A, Uehara T, Takeda K, Kunisada T, Ochiya T, and Ozaki T.
  • Organizer: Connective Tissue Oncology Society
  • Place of Presentation: ソルトレイクシティ（アメリカ）
  • Year and Date: 2015-11-04
  • Int'l Joint Research
• [Presentation] プロテオーム解析によるがん個別化医療のためのバイオマーカー開発 (2015)
  • Author(s): 近藤 格
  • Organizer: 第53回日本癌治療学会
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2015-10-29
• [Presentation] 骨肉腫患者血清における分泌型miRNAの特定と新規バイオマーカーとしての前臨床的検討 (2015)
  • Author(s): 藤原智洋, 魚谷弘二, 吉田 晶, 武田 健, 国定俊之, 根津 悠, 川井 章, 落谷孝広, 尾﨑敏文
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場（富山県）
  • Year and Date: 2015-10-22
• [Presentation] RPN2は骨肉腫の様々な悪性形質を制御し新規予後因子として機能する (2015)
  • Author(s): 藤原智洋, 川井 章, 落谷孝広, 尾﨑敏文.
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県）
  • Year and Date: 2015-10-08
• [Presentation] Cancer proteomics in the era of precision medicine (2015)
  • Author(s): Tadashi Kondo
  • Organizer: 2015年度・年会Human Proteome Organization
  • Place of Presentation: バンクーバー（カナダ）
  • Year and Date: 2015-09-27
  • Int'l Joint Research
• [Presentation] プロテオーム解析によるバイオマーカー開発におけるmRNAデータを用いたメタ解析 (2015)
  • Author(s): 近藤 格
  • Organizer: 第66回日本電気泳動学会
  • Place of Presentation: 東京工科大学蒲田キャンパス(東京）
  • Year and Date: 2015-09-04
• [Presentation] Rare Cancer Research in National Cancer Center (2015)
  • Author(s): Tadashi Kondo
  • Organizer: 第4回International Symposium of Sort-tissue Sarcoma of Upper Extrimity
  • Place of Presentation: 上海（中国）
  • Year and Date: 2015-08-17
  • Int'l Joint Research / Invited
• [Presentation] 粘液型脂肪肉腫の円形細胞成分に由来するmicroRNAおよび標的遺伝子の同定と悪性化形質に関与する分子メカニズムの解明 (2015)
  • Author(s): 根津 悠, 萩原啓太郎, 藤原智洋, 川井 章, 川端佑介, 松尾光祐, 齋藤知行, 落谷孝広.
  • Organizer: 第48回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: かがわ国際会議場(香川)
  • Year and Date: 2015-07-09
• [Presentation] 希少がんを研究するということ (2015)
  • Author(s): 近藤 格
  • Organizer: 第11回日本臨床プロテオーム研究会
  • Place of Presentation: 国際研究交流会館（東京）
  • Year and Date: 2015-05-23
• [Presentation] 悪性骨軟部腫瘍診療の集約化 (2015)
  • Author(s): 川井 章
  • Organizer: 第88回日本整形外科学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
  • Year and Date: 2015-05-21
• [Presentation] microRNA-133aとその標的遺伝子は骨肉腫がん幹細胞分画の悪性形質を制御し骨肉腫患者の新規予後因子として機能する (2015)
  • Author(s): 藤原智洋, 小坂展慶, 高橋陵宇, 竹下文隆, 吉田朗彦, 窪田大介, 小林英介, 落谷孝広, 川井 章, 尾﨑敏文.
  • Organizer: 第88回日本整形外科学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
  • Year and Date: 2015-05-21
• [Presentation] 日本発新規microRNA阻害核酸製剤Synthetic Tough Decoyを用いた骨肉腫に対する前臨床的検討. (2015)
  • Author(s): 藤原智洋, 根津 悠, 吉田 晶, 魚谷弘二, 武田 健, 国定俊之, 伊庭英夫, 伊藤 博, 尾﨑敏文, 落谷孝広, 川井 章.
  • Organizer: 第88回日本整形外科学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
  • Year and Date: 2015-05-21
• [Presentation] Casting doubt on conventional approach to cancer biomarker development by proteomics (2015)
  • Author(s): Tadashi Kondo
  • Organizer: Proteomic Forum 2015
  • Place of Presentation: Berlin, Germany,
  • Year and Date: 2015-03-23 – 2015-03-25
  • Invited
• [Presentation] 検出・診断・治療の最前線 “miRNA診断 (2015)
  • Author(s): 落谷孝広
  • Organizer: 第1回COINS国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-02-27
• [Presentation] 希少がんの基礎研究 (2015)
  • Author(s): 落谷孝広
  • Organizer: 第1回国産がん研究シンポジウム「希少がん〜望ましい医療・研究体制〜」
  • Place of Presentation: 東京
  • Year and Date: 2015-02-13
• [Presentation] 放射線治療のみで局所療法を行った骨盤原発ユーイング肉腫の長期予後の検討 (2014)
  • Author(s): 細野亜古,川井 章
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] マイクロRNAによるがんの革新的診断と治療 (2014)
  • Author(s): 落谷孝広
  • Organizer: メディバイオ事業研究会 第7回講演会
  • Place of Presentation: 山口 ,宇部
  • Year and Date: 2014-11-18
• [Presentation] 創薬研究に於ける動物治療モデルの意義と核酸・再生医療への応用 (2014)
  • Author(s): 落谷孝広
  • Organizer: 第35回動物臨床医学会記念年次大会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-16
• [Presentation] 我が国の革新的がん診断・治療技術の最前線 (2014)
  • Author(s): 落谷孝広
  • Organizer: For the Better Forum 2014
  • Place of Presentation: 東京
  • Year and Date: 2014-11-12
• [Presentation] 蛍光二次元電気泳動法を用いたがんのバイオマーカー開発 (2014)
  • Author(s): 近藤格
  • Organizer: 第65回日本電気泳動学会
  • Place of Presentation: 神奈川, 横浜
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] Proteomics identified over-expression of SET proto-oncogene product and possible therapeutic utility of protein phosphatase 2A in alveolar soft part sarcoma. (2014)
  • Author(s): Daisuke Kubota, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
  • Organizer: Human Proteome Organization
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-10-05 – 2014-10-08
• [Presentation] がん細胞が分泌する新規体液診断マーカー：エクソソーム (2014)
  • Author(s): 落谷孝広
  • Organizer: 第34回日本分子腫瘍マーカー研究会
  • Place of Presentation: 神奈川, 横浜
  • Year and Date: 2014-09-24
• [Presentation] Biomarker development for sarcoma toward personalized medicine (2014)
  • Author(s): Tadashi Kondo
  • Organizer: 19th World Congress on Advances in Oncology
  • Place of Presentation: Athena, Greek
  • Year and Date: 2014-09-09 – 2014-09-11
  • Invited
• [Presentation] Proteomics toward personalized treatment of sarcoma (2014)
  • Author(s): Tadashi Kondo, Akira Kawai
  • Organizer: Siena Meeting
  • Place of Presentation: Siena, Italy
  • Year and Date: 2014-08-31 – 2014-09-04
• [Presentation] 骨・軟部腫瘍（肉腫） (2014)
  • Author(s): 川井 章
  • Organizer: 第52回日本癌治療学会学術集会
  • Place of Presentation: 福島
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] エクソソームやmiRNAをターゲットとしたがん治療戦略 (2014)
  • Author(s): 落谷孝広
  • Organizer: 第１回緩和医療薬学研究会
  • Place of Presentation: 神奈川, 湯河原
  • Year and Date: 2014-08-24
  • Invited
• [Presentation] 肉腫（サルコーマ） (2014)
  • Author(s): 川井 章
  • Organizer: AKIBA Cancer Forum 2014
  • Place of Presentation: 東京, 秋葉原
  • Year and Date: 2014-08-09
• [Presentation] プロテオーム解析によるがんバイオマーカー開発の常識を疑う (2014)
  • Author(s): 近藤格
  • Organizer: 日本プロテオーム学会年会
  • Place of Presentation: 茨城, 筑波
  • Year and Date: 2014-07-17 – 2014-07-18
• [Presentation] エクソソームによる新しいシグナル伝達機構の解明 (2014)
  • Author(s): 落谷孝広
  • Organizer: 第79回日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 北海道, 札幌
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] Ewing Sarcoma(ESFT) (2014)
  • Author(s): 川井 章
  • Organizer: 8th SIOP Asia Congress
  • Place of Presentation: Seoul , ｋorea
  • Year and Date: 2014-04-17 – 2014-04-19
• [Book] 希少がんを研究するということ (2016)
  • Author(s): 近藤格
  • Total Pages: 67
  • Publisher: Clinic Magazin
• [Book] プロテオーム解析によるがんバイオマーカー開発 (2016)
  • Author(s): 近藤格
  • Total Pages: 187
  • Publisher: 生物試料分析
• [Book] プロテオーム解析によるがんのバイオマーカー開発 (2016)
  • Author(s): 近藤格
  • Total Pages: 863
  • Publisher: 血液内科
• [Book] 悪性胸膜中皮腫のプロテオーム解析 (2016)
  • Author(s): 近藤格
  • Total Pages: 775
  • Publisher: 医学のあゆみ
• [Book] こどものがんと治療 (2016)
  • Author(s): 細野亜古
  • Total Pages: 112
  • Publisher: メディカ出版
• [Book] 肉腫化学療法マスタークラス (2015)
  • Author(s): 川井 章
  • Total Pages: 367
  • Publisher: 南山堂
• [Book] Novel prognostic biomarker, pfetin, in gastrointestinal stromal tumors: proteomics study (2015)
  • Author(s): Kondo T
  • Publisher: General Methods in Biomarker Research and Their Applications.
• [Book] Epigenetic Biomarkers and Diagnostics, 1st Edition. (2015)
  • Author(s): Fujiwara T, Fujita Y, Nezu Y, Kawai A, Ozaki T, Ochiya T.
  • Publisher: Academic Press, Inc.
• [Book] 臨床プロテオミクス (2014)
  • Author(s): 近藤格
  • Publisher: 医学のあゆみ
• [Book] Challenges and strategies for pulmonary delivery of microRNA-based therapeutics. (2014)
  • Author(s): Fujita Y, Kuwano K, Ochiya T.
  • Publisher: Springer
• [Book] The potential role of MicroRNA-based therapy for lung cancer stem cells. (2014)
  • Author(s): Fujita Y, Kuwano K, OchiyaT.
  • Publisher: Springer