|Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|Outline of Final Research Achievements
When using Colloidal Platinum Nanoparticls (CPN) as a primer solution, bonding to caries-affected dentin with Cleafil SE2 did not show improvement in bond strength. Two step dentin conditioner created with the combination of acid and CPN was effective for improving bond strength but affected by the moisture of dentin to be bonded.
When CPN was applied to Hela cells, immunoblot demonstrated the expression of Chromogranin A and its related proteins, showing the level of the expression was in proportion to the concentration of CPN. So far, it is not clear the machanizm of the expression of the antibacterial proteins. Future study is important disclose the mechanizm of the phenomenon.