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Surveillance of Chlamydia and their diversity in wildlife and livestock in Ghana

Research Project

Project/Area Number 26304039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Veterinary medical science
Research InstitutionNational Institute of Health Sciences (2018)
Gifu University (2014-2017)

Principal Investigator

Ohya Kenji  国立医薬品食品衛生研究所, 衛生微生物部, 室長 (50402219)

Co-Investigator(Kenkyū-buntansha) 福士 秀人  岐阜大学, 応用生物科学部, 教授 (10156763)
中尾 亮  北海道大学, 獣医学研究院, 准教授 (50633955)
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords感染症 / 野生動物 / 家畜 / クラミジア / アフリカ / 獣医学 / 病原体 / 齧歯類 / 細菌 / アナプラズマ / 畜産学 / 衛生 / ゲノム / ガーナ / 疫学
Outline of Final Research Achievements

Chlamydia, one of the important pathogens in the medical and veterinary fields, exhibits broad host range and pathogenesis in their host. In this study, we carried out surveillance and analyzed diversity of chlamydia species among wildlife and livestock (local breeds) in Ghana. We revealed that circulation of chlamydia among wild birds and local livestock reared extensively around an inland foothill of Ghana, and that chlamydia resides latently in extensively reared local livestock in a farm in Accra. In addition to chlamydia, we conducted microbiological inspection of grasscutters which is a giant rodent on the way of domestication, to examine whether they possess pathogens for human and other livestock and to compare their intestinal flora with other livestock. Through this study, we think we could gain a lot of knowledge with potential to improve public health and animal hygiene in this country.

Academic Significance and Societal Importance of the Research Achievements

本課題では、クラミジアの自然界における存在様式について、病原体ゲノム、動物遺伝学および生態学の専門家と協力し、多方面からの解析を実施することができた。さらに、生物多様性の宝庫というガーナの特性を活かし、国内では実施不可能な包括的な解析を行うことができた。本課題の遂行中に得られた知見は、他の病原体にも応用することができ、特に家畜の抗酸菌症、節足動物媒介感染症では多くの成果が得られた。また、ガーナで家畜化が試みられている齧歯類グラスカッターの保有微生物叢の解析も可能となり、飼養効率向上を目指した後続研究が行われている。以上のように、国内外の公衆衛生、家畜衛生向上に繋がる成果が得られたと考えている。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (44 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (6 results) Journal Article (22 results) (of which Int'l Joint Research: 14 results,  Peer Reviewed: 20 results,  Open Access: 13 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Invited: 1 results) Book (4 results) Funded Workshop (2 results)

  • [Int'l Joint Research] University of Ghana(ガーナ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Makerere University(ウガンダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Ghana(ガーナ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Makerere University(ウガンダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ガーナ大学(ガーナ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Dept. of Animal Science, Univ. of Ghana/Dept. of Animal Biology and Conservation(Ghana)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Native SAG1 in Toxoplasma gondii lysates is superior to recombinant SAG1 for serodiagnosis of T. gondii infections in chickens2019

    • Author(s)
      Appiah-Kwarteng C, Saito T, Toda N, Kitoh K, Nishikawa Y, Adenyo C, Kayang B, Owusu EO, Ohya K, Inoue-Murayama M, Kawahara F, Nagamune K, Takashima Y
    • Journal Title

      Parasitol Int

      Volume: 69 Pages: 114-120

    • DOI

      10.1016/j.parint.2019.01.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular detection of Rickettsia felis in dogs, rodents and cat fleas in Zambia2019

    • Author(s)
      Moonga Lavel Chinyama、Hayashida Kyoko、Nakao Ryo、Lisulo Malimba、Kaneko Chiho、Nakamura Ichiro、Eshita Yuki、Mweene Aaron S.、Namangala Boniface、Sugimoto Chihiro、Yamagishi Junya
    • Journal Title

      Parasites & Vectors

      Volume: 12 Issue: 1 Pages: 168-168

    • DOI

      10.1186/s13071-019-3435-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Potential role of dogs as sentinels and reservoirs for piroplasms infecting equine and cattle in Riyadh City, Saudi Arabia2019

    • Author(s)
      Salim Bashir、Alanazi Abdullah D.、Omori Ryosuke、Alyousif Mohamed S.、Alanazi Ibrahim O.、Katakura Ken、Nakao Ryo
    • Journal Title

      Acta Tropica

      Volume: 193 Pages: 78-83

    • DOI

      10.1016/j.actatropica.2019.02.029

    • NAID

      120006843068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure2019

    • Author(s)
      Chatanga Elisha、Mosssad Ehab、Abdo Abubaker Hazem、Amin Alnour Sondos、Katakura Ken、Nakao Ryo、Salim Bashir
    • Journal Title

      Acta Tropica

      Volume: 191 Pages: 128-132

    • DOI

      10.1016/j.actatropica.2018.12.041

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phenotypic and genotypic analyses of antimicrobial- resistant bacteria in livestock in Uganda.2018

    • Author(s)
      Okubo, T., Yossapol, M., Maruyama, F., Wampande, E. M., Kakooza, S., Ohya, K., Tsuchida, S., Asai, T., Kabasa, J. D. and Ushida, K.
    • Journal Title

      Transboundary Emerg. Dis.

      Volume: 66 Issue: 1 Pages: 317-326

    • DOI

      10.1111/tbed.13024

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft genome sequences of Mycolicibacter senuensis strain GF74 and Mycobacterium colombiense strain GF28 and GF76, isolated from a swine farm in Japan.2018

    • Author(s)
      Ito, T., Sawai, K., Kawai, M., Nozaki, K., Otsu, K., Fukushi, H., Ohya, K. and Maruyama, F.
    • Journal Title

      Microbiol. Resour. Announc.

      Volume: 7 Issue: 10

    • DOI

      10.1128/mra.00936-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft genome sequence of Mycobacterium virginiense strain GF75, isolated from the mud of a swine farm in Japan2018

    • Author(s)
      Ito T, Maruyama F, Sawai K, Nozaki K, Otsu K, Ohya K
    • Journal Title

      Genome Announc.

      Volume: - Issue: 17

    • DOI

      10.1128/genomea.00362-18

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogenetic uniqueness of Mycobacterium avium subspecies hominissuis isolated from an abnormal pulmonary bovine case2018

    • Author(s)
      Yoshida Shiomi、Araki Tsubasa、Asai Tomohito、Tsuyuguchi Kazunari、Arikawa Kentaro、Iwamoto Tomotada、Nakajima Chie、Suzuki Yasuhiko、Ohya Kenji、Yanai Tokuma、Wada Takayuki、Yamamoto Taro
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 62 Pages: 122-129

    • DOI

      10.1016/j.meegid.2018.04.013

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan2018

    • Author(s)
      Oba M, Katayama Y, Tsuhiaka S, Omatsu T, Murata Y, Ohya K, Makino S, Nagai M, Mizutani T
    • Journal Title

      Japanese Journal of Veterinary Research

      Volume: 66 Issue: 1 Pages: 53-56

    • DOI

      10.14943/jjvr.66.1.53

    • NAID

      120006425630

    • ISSN
      0047-1917
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Involvement of multiple Chlamydia suis genotypes in porcine conjunctivitis.2018

    • Author(s)
      Chahota R, Ogawa H, Ohya K, Yamaguchi T, Everett KD, Fukushi H
    • Journal Title

      Transbound. Emerg. Dis.

      Volume: 65 Issue: 1 Pages: 272-277

    • DOI

      10.1111/tbed.12645

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia2018

    • Author(s)
      Qiu Yongjin、Kaneko Chiho、Kajihara Masahiro、Ngonda Saasa、Simulundu Edgar、Muleya Walter、Thu May J、Hang’ombe Bernard、Katakura Ken、Takada Ayato、Sawa Hirofumi、Simuunza Martin、Nakao Ryo
    • Journal Title

      Ticks and Tick-borne Diseases

      Volume: 印刷中 Issue: 4 Pages: 988-995

    • DOI

      10.1016/j.ttbdis.2018.03.025

    • NAID

      120006626572

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents2018

    • Author(s)
      Chitanga Simbarashe、Simulundu Edgar、Simuunza Martin、Changula Katendi、Qiu Yongjin、Kajihara Masahiro、Nakao Ryo、Syakalima Michelo、Takada Ayato、Mweene S Aaron、Mukaratirwa Samson、Hang’ombe Bernard
    • Journal Title

      Parasites & Vectors

      Volume: 11 Issue: 1 Pages: 40-40

    • DOI

      10.1186/s13071-018-2629-7

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia2018

    • Author(s)
      Vlahakis Pipina、Chitanga Simbarashe、Simuunza Martin、Simulundu Edgar、Qiu Yongjin、Changula Katendi、Chambaro Herman、Kajihara Masahiro、Nakao Ryo、Takada Ayato、Mweene Aaron
    • Journal Title

      Ticks and Tick-borne Diseases

      Volume: 9 Issue: 1 Pages: 39-43

    • DOI

      10.1016/j.ttbdis.2017.10.010

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetic homogeneity of goat malaria parasites in Asia and Africa suggests their expansion with domestic goat host.2018

    • Author(s)
      Kaewthamasorn M, Takeda M, Saiwichai T, Gitaka JN, Tiawsirisup S, Imasato Y, Mossaad E, Sarani A, Kaewlamun W, Channumsin M, Chaiworakul S, Katepongpun W, Teeveerapunya S, Panthong J, Mureithi DK, Bawm S, Htun LL, Win MM, Ismail AA, Ibrahim AM, Suganuma K, Hakimi H, Nakao R, Katakura K, Asada M, Kaneko O
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 5827-5827

    • DOI

      10.1038/s41598-018-24048-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia.2018

    • Author(s)
      Vlahakis PA, Chitanga S, Simuunza MC, Simulundu E, Qiu Y, Changula K, Chambaro HM, Kajihara M, Nakao R, Takada A, Mweene AS.
    • Journal Title

      Ticks Tick Bonrne Dis.

      Volume: 9 Pages: 39-43

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers.2017

    • Author(s)
      Moendeg KJ, Angeles JMM, Nakao R, Leonardo LR, Fontanilla IKC, Goto Y, Kirinoki M, Villacorte EA, Rivera PT, Inoue N, Chigusa Y, Kawazu SI
    • Journal Title

      PLoS Negl. Trop. Dis.

      Volume: 11 Issue: 7 Pages: e0005749-e0005749

    • DOI

      10.1371/journal.pntd.0005749

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan2017

    • Author(s)
      Izume S, Kirisawa R, Ohya K, Ohnuma A, Kimura T, Omatsu T, Katayama Y, Mizutani T, Fukushi H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 1 Pages: 206-212

    • DOI

      10.1292/jvms.16-0506

    • NAID

      130005296375

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ガーナ共和国の家畜飼育の現状と在来家畜について2016

    • Author(s)
      大屋 賢司
    • Journal Title

      日生研たより

      Volume: 62 Pages: 92-96

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Draft genome sequences of three strains of Ehrlichia ruminantium, a tick-borne pathogen of ruminants, isolated from Zimbabwe, The Gambia, and Ghana2016

    • Author(s)
      Nakao R, Jongejan F, Sugimoto C
    • Journal Title

      Genome Announc

      Volume: 4 Issue: 3

    • DOI

      10.1128/genomea.00453-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Genetic and antigenic analysis of <i>Chlamydia pecorum</i> strains isolated from calves with diarrhea2015

    • Author(s)
      Ohtani A, Kubo M, Shimoda H, Ohya K, Iribe T, Ohishi D, Endoh D, Omatsu T, Mizutani T, Fukushi H, Maeda K
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 7 Pages: 777-782

    • DOI

      10.1292/jvms.14-0585

    • NAID

      130005089948

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Equine herpesvirus type 1 tegument protein VP22 is not essential for pathogenicity in a hamster model, but is required for efficient viral growth in cultured cells2015

    • Author(s)
      Okada A et al.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 10 Pages: 1293-1297

    • DOI

      10.1292/jvms.14-0648

    • NAID

      130005107586

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オウム病2014

    • Author(s)
      福士秀人
    • Journal Title

      公衆衛生情報

      Volume: 44 Pages: 20-21

    • Related Report
      2014 Annual Research Report
  • [Presentation] 西アフリカの大型齧歯類グラスカッターと反芻家畜の腸内菌叢比較2018

    • Author(s)
      大屋 賢司,片山 幸枝,大松 勉,高島 康弘,澤井 宏太郎,小川 寛人,Chris Adenyo, Boniface Kayang, 水谷 哲也,福士 秀人,村山 美穂
    • Organizer
      第161回 日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ガーナにおけるグラスカッター飼育プロジェクト2017

    • Author(s)
      大屋 賢司、高島 康弘、小川 寛人、大松 勉、水谷 哲也、川端 寛樹、Christpher Adenyo、Boniface Kayang、村山 美穂
    • Organizer
      第54回 日本アフリカ学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 西アフリカの大型齧歯類グラスカッターの保有する微生物について2017

    • Author(s)
      大屋 賢司、高島 康弘、小川 寛人、大松 勉、片山 幸枝、水谷 哲也、佐藤(大 久保) 梢、川端 寛樹、松本 干城、中尾 亮、福士 秀人、 Christopher Adenyo、Boniface B. Kayang、村山 美穂
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 動物に存在する多様なクラミジアについて2017

    • Author(s)
      大屋 賢司
    • Organizer
      第35回 日本クラミジア研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ガーナの農場で飼育される山羊・羊(地域種)における病原体保有状況2016

    • Author(s)
      大屋賢司、岡田彩加、下里徳宏、鈴木陽介、菅沼彰太、Chris ADENYO, Boniface KAYANG, 村山美穂、福士秀人
    • Organizer
      第159回 日本獣医学会学術集会
    • Place of Presentation
      藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 流産の発生した豚農場におけるクラミジアの分布状況2016

    • Author(s)
      鈴木陽介、大屋 賢司、菅沼彰太、福士 秀人
    • Organizer
      第159回 日本獣医学会学術集会
    • Place of Presentation
      藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 農場で飼育される和牛(黒毛和種)糞便からのChlamydia pecorumの検出2015

    • Author(s)
      菅沼彰太ら
    • Organizer
      第33回 日本クラミジア研究会学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガーナ共和国アファジャト山麓に生息する野鳥および家畜が保有するクラミジアの疫学調査2015

    • Author(s)
      安田早織ら
    • Organizer
      第158回 日本獣医学会学術集会
    • Place of Presentation
      十和田市
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガーナ共和国アファジャト山麓の野鳥、家畜におけるクラミジア保有状況調査2015

    • Author(s)
      大屋賢司ら
    • Organizer
      第52回 日本アフリカ学会学術退会
    • Place of Presentation
      犬山市
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガーナ共和国の山麓に生息する野鳥および家畜に分布するクラミジアの疫学調査2015

    • Author(s)
      大屋賢司、福士秀人
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜市
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Book] 猫クラミジア症,動物病院スタッフのための感染症ガイド2019

    • Author(s)
      大屋 賢司
    • Total Pages
      2
    • Publisher
      緑書房
    • ISBN
      9784895313681
    • Related Report
      2018 Annual Research Report
  • [Book] クラミジア門クラミジア綱,獣医微生物学(第4版)2018

    • Author(s)
      大屋 賢司
    • Total Pages
      6
    • Publisher
      文永堂
    • ISBN
      9784830032707
    • Related Report
      2018 Annual Research Report
  • [Book] 猫クラミジア症,臨床獣医師のための犬と猫の感染症診療2018

    • Author(s)
      大屋 賢司
    • Total Pages
      6
    • Publisher
      緑書房
    • ISBN
      9784895313513
    • Related Report
      2018 Annual Research Report
  • [Book] クラミジア・宿主間相互作用(偏性細胞内寄生性) 病原細菌・ウイルス図鑑2017

    • Author(s)
      大屋 賢司
    • Total Pages
      8
    • Publisher
      北海道大学出版会
    • ISBN
      9784832982291
    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] Novel domestication of large rodent “grasscutter” in Ghana.2018

    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] Ghana Grasscutter Project Seminar2015

    • Place of Presentation
      University of Ghana, Accra, Ghana
    • Year and Date
      2015-10-01
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2022-05-18  

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