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Structure-based design of nitrification inhibitor targeting hydroxylamine oxidoreductases of ammonia-oxidizing bacteria

Research Project

Project/Area Number 26310317
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section特設分野
Research Field Food Cycle Research
Research InstitutionNational Agriculture and Food Research Organization (2016)
National Institute of Agrobiological Sciences (2014-2015)

Principal Investigator

Toshimasa Yamazaki  国立研究開発法人農業・食品産業技術総合研究機構, 高度解析センター・生体高分子解析チーム, チーム長 (40360458)

Co-Investigator(Kenkyū-buntansha) 藤原 健智  静岡大学, 理学研究科, 教授 (80209121)
Co-Investigator(Renkei-kenkyūsha) FUJIMOTO ZUI  国立研究開発法人農業・食品産業技術総合研究機構, 高度解析センター 生体高分子解析チーム, 上級研究員 (20370679)
Project Period (FY) 2014-07-18 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Keywords環境調和型農林水産 / 地球温暖化ガス排出削減 / 分子標的型硝化抑制剤 / 構造ベース創農薬 / 分子標的硝化抑制剤
Outline of Final Research Achievements

About 50% of the nitrogen (N)-fertilizer applied into cropping systems is not absorbed by plants, but lost to the environment as ammonia (NH3), nitrate (NO3-), and nitrous oxide (N2O), a greenhouse gas with a global warming potential of 300 times greater than that of CO2, raising agricultural production costs and contributing to pollution and climate changes. These N losses are caused by nitrification, the oxidation of NH3 to NO3-, catalyzed by the ammonia- and nitrite-oxidizing bacteria (AOB and NOB). To reduce nitrification and N2O emissions, we have developed seed compounds of novel nitrification inhibitors targeting hydroxylamine oxidoreductase, one of the two key enzymes of AOB, by utilizing the structure-based drug design method.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Products Report

Research Products

(9 results)

All 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Optimized inhibition assays reveal different inhibitory responses of hydroxylamine oxidoreductases from beta- and gamma-proteobacterial ammonium-oxidizing bacteria2016

    • Author(s)
      Yuki Nishigaya, Zui Fujimoto, Toshimasa Yamazaki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 476 Pages: 127-133

    • DOI

      10.1016/j.bbrc.2016.05.041

    • NAID

      120006358564

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ヒドロキシルアミン酸化還元酵素を標的とした硝化抑制剤の構造ベース創農薬2017

    • Author(s)
      西ヶ谷有輝、土屋渉、藤本瑞、山崎俊正
    • Organizer
      日本農薬学会42回大会
    • Place of Presentation
      愛媛大学(愛媛県・松山市)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structure analysis of target proteins applied to BNI action mechanism research2016

    • Author(s)
      Yuki Nishigaya, Toshimasa Yamazaki
    • Organizer
      BNI International Symposium
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 持続的農業に貢献する硝化抑制剤の創農薬2016

    • Author(s)
      西ヶ谷有輝、藤本瑞、土屋渉、山崎俊正
    • Organizer
      第7回スクリーニング学研究会
    • Place of Presentation
      タワーホール船堀(東京都・江戸川区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Revealing inhibition mechanism against hydroxylamine oxidoreductase (HAO)2015

    • Author(s)
      Yuki Nishigaya, Toshimasa Yamazaki
    • Organizer
      International BNI workshop 2015
    • Place of Presentation
      Tsukuba
    • Year and Date
      2015-03-02 – 2015-03-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 構造解析と新規阻害剤を利用したアンモニア酸化細菌由来ヒドロキシルアミン酸化還元酵素に対する硝化抑制剤ターゲットとしてのバリデーション2014

    • Author(s)
      西ヶ谷有輝、熊谷美穂、土屋渉、藤本瑞、藤原健智、山崎俊正
    • Organizer
      生化学会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 酸化還元酵素の活性測定方法2017

    • Inventor(s)
      山崎俊正、西ヶ谷有輝
    • Industrial Property Rights Holder
      山崎俊正、西ヶ谷有輝
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-01-18
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 酸化還元酵素の活性測定方法2017

    • Inventor(s)
      山崎俊正, 西ヶ谷有輝
    • Industrial Property Rights Holder
      国立研究開発法人農業・食品産業技術総合研究機構
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-01-18
    • Acquisition Date
      2018-01-12
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] テトラゾリウム塩の存在下でヒドロキシルアミン酸化還元酵素と接触させることを含む、ヒドロキシルアミン酸化還元酵素の活性測定方法2016

    • Inventor(s)
      山崎俊正、西ヶ谷有輝
    • Industrial Property Rights Holder
      山崎俊正、西ヶ谷有輝
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-010983
    • Filing Date
      2016-01-22
    • Related Report
      2015 Annual Research Report

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Published: 2014-09-09   Modified: 2019-02-27  

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