Clarification of role of photorespiration in plant response to ozone

• Project/Area Number: 26340033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: SAJI Hikaru 国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 上級主席研究員 (00178683)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: オゾン / 光呼吸 / 遺伝子 / シロイヌナズナ / グリコール酸オキシダーゼ / 酸化的ストレス / 強光 / 突然変異体 / グリコール酸

Outline of Final Research Achievements

In order to get information on the mechanism of plant tolerance to ozone, we carried out genetic and physiological analyses on the isolated Arabidopsis ozone-sensitive mutant. We obtained results that the gene responsible for the mutant's ozone sensitivity encodes a photorespiratory enzyme, glycolate oxidase, and the ozone sensitivity was due to the reduced enzyme activity. This mutant also exhibited high sensitivity against sulfur dioxide and a long-term high-intensity light. Two mutant lines that have defects in other photorespiratory enzymes also exhibited high ozone sensitivities. These results suggest that intact photorespiratory flow is neccessary for the plant tolerance to ozone as well as other stress factors.

Research Products

• [Int'l Joint Research] ロストック大学(ドイツ)
• [Int'l Joint Research] ドラヴィディアン大学(インド)
• [Journal Article] Ozone-sensitive Arabidopsis mutants with deficiencies in photorespiratory enzymes (2017)
  • Author(s): Saji, S., Bathula, S., Kubo, A., Tamaoki, M., Aono, M., Sano, T., Tobe, K., Timm, S., Bauwe, H., Nakajima, N., Saji, H.
  • Journal Title: Plant and Cell Physiology Volume: 印刷中
  • DOI: 10.1093/pcp/pcx027
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] シロイヌナズナ光呼吸系欠損突然変異体のオゾン応答 (2016)
  • Author(s): 佐治光，佐治章子，Bathula S.，久保明弘，玉置雅紀，青野光子，佐野友春，戸部和夫，中嶋信美
  • Organizer: 第57回大気環境学会年会
  • Place of Presentation: 北海道大学工学部C棟（北海道）
  • Year and Date: 2016-09-08
• [Presentation] Isolation and characterization of an ozone-sensitive Arabidopsis mutant with deficiencies in glycolate oxidases (2015)
  • Author(s): S. Saji, S. Bathula, A. Kubo, M. Tamaoki, M. Aono, T. Sano, K. Tobe, N. Nakajima, H. Saji
  • Organizer: The 1st Asian Air Pollution Workshop
  • Place of Presentation: 東京大学農学部7号館B棟（東京都）
  • Year and Date: 2015-10-31
  • Int'l Joint Research
• [Presentation] オゾン感受性を示すシロイヌナズナの光呼吸系突然変異体の性質 (2015)
  • Author(s): 佐治光, 佐治章子, Bathula Srinivas, 久保明弘, 玉置雅紀, 青野光子, 佐野友春, 戸部和夫, 中嶋信美
  • Organizer: 第56回大気環境学会年会
  • Place of Presentation: 早稲田大学西早稲田キャンパス52号館（東京都）
  • Year and Date: 2015-09-15
• [Presentation] An ozone-sensitive Arabidopsis mutant with deficiencies in glycolate oxidases (2015)
  • Author(s): Saji S., Bathula S., Kubo A., Tamaoki M., Aono M., Sano T., Tobe K., Nakajima N., Saji H.
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス1号館（東京）
  • Year and Date: 2015-03-16
• [Presentation] 植物のオゾン耐性における光呼吸の関与 (2014)
  • Author(s): 佐治光、佐治章子、Bathula S.、久保明弘、玉置雅紀、青野光子、戸部和夫、中嶋信美
  • Organizer: 第55回大気環境学会年会
  • Place of Presentation: 愛媛大学城北キャンパス共通教育棟（松山）
  • Year and Date: 2014-09-17