Molecular mechanism of heme-regulated inhibitor (HRI) under stress conditions
Project/Area Number |
26340041
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | Fukushima Medical University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | ヘム / X線結晶構造解析 / 結晶構造解析 / グロビン / ヒ素 / ヘム調節インヒビター / タンパク質間相互作用 |
Outline of Final Research Achievements |
Heme-regulated eukaryotic initiation factor 2α (eIF2α) kinase (HRI), functions in response to heme concentration. Under stress conditions, HRI forms stress granules and down-regulates protein synthesis. The molecular mechanism of HRI remained to be established. In the present study, we demonstrate that HRI degradation is inhibited by bortezomib in cultured cells. Using pull-down assay, the interaction between HRI and OGFOD1 are observed. Furthermore, to solve the crystal structure of HRI, insect cell expression systems are constructed. In addition, we also elucidate the mechanism of ligand recognition of DgcO from Escherichia coli. DgcO, which is also known as YddV or DosC, consists of globin domain, middle domain, and diguanylate cyclase domain. We solved the crystal structure of globin domain of DgcO in the presence of various ligands.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] Hoki myoglobin with unusual autoxidation behavior: Comparative analysis of native protein and H60V mutant2016
Author(s)
Kobayashi, G., Igarashi, J., Rei, M., Nishiyama, N., Mizuguchi, T., Matsuoka, A.
Organizer
The 22nd International Congress of Zoology and the 87th meeting of Zoological Society of Japan
Place of Presentation
宜野湾
Year and Date
2016-11-17
Related Report
Int'l Joint Research
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[Presentation] Stability properties of fish myoglobin from hoki (Macruronus magellanicus)2015
Author(s)
Kobayashi, G., Nishiyama, N., Endo, Y., Mizuguchi, T., Igarashi, J., and Matsuoka, A.
Organizer
The 9th International Congress of Comparative Physiology and Biochemistry
Place of Presentation
Krakow, Poland
Year and Date
2015-08-23
Related Report
Int'l Joint Research
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