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Development of technology for degradation of formaldehyde in liquid and gas phases using micro-organism

Research Project

Project/Area Number 26340065
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionWakayama National College of Technology

Principal Investigator

YONEMITSU Hiroshi  和歌山工業高等専門学校, 物質工学科, 教授 (20290778)

Co-Investigator(Kenkyū-buntansha) 岸本 昇  和歌山工業高等専門学校, 物質工学科, 教授 (50280433)
奥野 祥治  和歌山工業高等専門学校, 物質工学科, 准教授 (60458073)
土井 正光  和歌山工業高等専門学校, 物質工学科, 教授 (30217608)
楠部 真崇  和歌山工業高等専門学校, 物質工学科, 准教授 (40403761)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
KeywordsFormaldehyde degradation / Methlobacterium sp. FD1 / formaldehyde dismutase / gene cloning / recombinant E. coli / immobilized cells / ホルムアルデヒド / ホルムアルデヒド分解菌 / 固定化菌体 / バイオリアクター / 連続操作 / ホルムアルデヒドジスムターゼ / ホルムアルデヒド分解除去技術 / ホルムアルデヒド分解酵素遺伝子
Outline of Final Research Achievements

In present study, the formaldehyde dismutase gene (1,203 bp) was cloned from the genomic DNA of Methylobacterium sp.FD1. The resting Escherichia coli DE3 cells that were transformed with the FD1 formaldehyde dismutase gene degraded high concentrations of formaldehyde (2.0%) and produced formic acid and methanol that were molar equivalents of one-half of the degraded formaldehyde. The lyophilized cells of the recombinant E. coli also degraded high concentrations of formaldehyde. Formaldehyde (0.1%) was almost degraded in cloth-immobilized FD1 cell-bioreactor on the continuous operation. Alginate calcium immobilized FD1 cells degraded formaldehyde in gas phase. From the above results, Methylobacterium sp. FD1 cells and E. coli transformed with FD1 formaldehyde dismutase gene are good candidates for agents to degrade formaldehyde in liquid and gas phase.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(4 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Biodegradation of high concentrations of formaldehyde by lyophilized cells of Methylobacterium sp. FD12016

    • Author(s)
      Hiroshi Yonemitsu, Emi Shiozaki, Fumina Hitotsuda, Noboru Kishimoto, Yoshiharu Okuno, Kazuki Nakagawa and Koji Hori
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Pages: 2264-2270

    • DOI

      10.1080/09168451.2016.1214535

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] M. fujisawaense FD-1株由来ホルムアルデヒド分解酵素の反応速度論的検討2017

    • Author(s)
      西井太郎, 岸本昇, 米光裕
    • Organizer
      第19回化学工学会学生発表会(豊中大会)研究発表講演要旨集
    • Place of Presentation
      大阪大学豊中キャンパス
    • Related Report
      2016 Annual Research Report
  • [Presentation] Methylobacterium sp. FD1株のホルムアルデヒドジスムターゼ遺伝子の単離と大腸菌での発現2016

    • Author(s)
      米光裕、菊地悠太、瀨田寛行、藏富大智、岸本昇、奥野祥治
    • Organizer
      日本農芸化学会2016年度大会(札幌)
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 遺伝子組換え大腸菌によるホルムアルデヒド分解2016

    • Author(s)
      瀬田 寛行、米光 裕、岸本 昇、奥野 祥治
    • Organizer
      第18回化学工学会学生発表会(福岡大会)
    • Place of Presentation
      福岡大学
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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