• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation of Antimicrobial Susceptibility and Antibiotic Resistance Gene Transfer of Enterococci in Water Environment

Research Project

Project/Area Number 26340085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental risk control and evaluation
Research InstitutionUniversity of Miyazaki

Principal Investigator

Suzuki Yoshihiro  宮崎大学, 工学部, 教授 (90264366)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords都市河川 / 薬剤耐性菌 / 腸球菌 / バンコマイシン / 流域拡散 / 薬剤耐性腸球菌 / 河川 / 実態調査 / 耐性遺伝子伝達
Outline of Final Research Achievements

VREs cause nosocomial infectious diseases in medical institutions, also exist in environmental water sources. In this study, the distribution of VRE and the resistance of enterococci isolates to various antibiotics were investigated in the urban river. The vanC1 and vanC2/3 were detected in the isolates from river water. Furthermore, the isolates containing vanC2/3 were confirmed in all sampling sites. For the next objective, the in vitro transfer experiments were performed with donor/recipient ratio of 1:1 under the following conditions: filter mating, broth mating, and autoclaved river water and sediment. Transconjugants were confirmed by determining their phenotypic and genotypic properties. The transfer frequencies of vancomycin resistance by filter mating experiments were 10-7 to 10-9 per recipient cell to three different enterococcus strains.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(21 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan2017

    • Author(s)
      Masateru Nishiyama, Yoshitoshi Ogura, Tetsuya Hayashi, Yoshihiro Suzuki.
    • Journal Title

      Water

      Volume: 9 Pages: 1-17

    • DOI

      10.3390/w9020079

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Distribution of antimicrobial resistant Salmonella in an urban river that flows through the provincial city of Miyazaki, Japan2016

    • Author(s)
      Suzuki Y, Ushijima M.
    • Journal Title

      Water and Environment Journal

      Volume: 30 Pages: 290-297

    • DOI

      10.1111/wej.12194

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Concentration of MS2 Phage in River Water by a Combined Ferric Colloid Adsorption and Foam Separation-Based Method, with Ms2 Phage Leaching from Ferric Colloid2016

    • Author(s)
      Suzuki Y, Kobayashi T, Nishiyama M, Kono T
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中 Issue: 2 Pages: 252-256

    • DOI

      10.1016/j.jbiosc.2016.01.003

    • NAID

      40020927741

    • ISSN
      1389-1723
    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temporal variability of genetic patterns and antibiotic resistance profiles of enterococci isolated from human faeces2016

    • Author(s)
      Nishiyama M, Shimauchi H, Suzuki Y
    • Journal Title

      Microbes and Environments

      Volume: 印刷中

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 下水と河川水における薬剤耐性腸球菌の存在実態とその比較2015

    • Author(s)
      西山正晃, 竹下友作, 鈴木祥広
    • Journal Title

      水環境学会誌

      Volume: 38 Pages: 57-65

    • NAID

      130004923017

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of Enterococcus faecium and Enterococcus faecalis as vanC-type vancomycin-resistant enterococci (VRE) from sewage and river water in the provincial city of Miyazaki, Japan2015

    • Author(s)
      Nishiyama T, Iguchi A, Suzuki Y
    • Journal Title

      ournal of Environmental Science and Health, Part A

      Volume: 50 Pages: 16-25

    • DOI

      10.1080/10934529.2015.964599

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 宮崎市内を流下する八重川上・下流における薬剤耐性大腸菌の薬剤耐性プロファイルの比較2016

    • Author(s)
      西村恵美, 西山正晃, 糠澤桂, 鈴木祥広
    • Organizer
      第53回環境工学研究フォーラム講演集
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 凝集と泡沫分離の複合プロセスによる河川水からの高効率DNA回収法の開発2016

    • Author(s)
      西山正晃, 今福夕貴, 寺西康太郎, 鈴木祥広
    • Organizer
      第53回環境工学研究フォーラム講演集
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] イオン化飛行型質量分析計(MALDI-TOF MS)による水環境中の大腸菌群の迅速同定2016

    • Author(s)
      鈴木祥広, 新名晃宜, 井口純
    • Organizer
      第19回日本水環境学会シンポジウム講演集
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水環境における腸球菌の薬剤耐性獲得・伝播ポテンシャルの評価2016

    • Author(s)
      西山正晃,鈴木祥広
    • Organizer
      第19回日本水環境学会シンポジウム講演集
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水環境における腸球菌の薬剤耐性遺伝子の伝播ポテンシャルに関する検討2016

    • Author(s)
      西山正晃, 鈴木祥広
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティとくしま(徳島県徳島市)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] イオン化飛行型質量分析計(MALDI-TOF MS)による大腸菌群の迅速かつ正確な同定手法の確立2016

    • Author(s)
      新名晃宜, 井口純, 鈴木祥広
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティとくしま(徳島県徳島市)
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] レクリエーションビーチの砂浜における細菌汚染の実態と起源調査2016

    • Author(s)
      寺西康太郎, 鈴木祥広
    • Organizer
      平成27年度日本水環境学会九州沖縄支部研究発表会
    • Place of Presentation
      佐賀大学(佐賀県佐賀市)
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 凝集と泡沫分離の複合プロセスによる河川水からの DNA の高効率濃縮法の開発.2016

    • Author(s)
      今福夕貴, 鈴木祥広
    • Organizer
      平成27年度日本水環境学会九州沖縄支部研究発表会
    • Place of Presentation
      佐賀大学(佐賀県佐賀市)
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 泡沫分離濃縮法を用いた河川水からの活性大腸菌回収法の検討2016

    • Author(s)
      上田卓矢, 鈴木祥広
    • Organizer
      平成27年度日本水環境学会九州沖縄支部研究発表会
    • Place of Presentation
      佐賀大学(佐賀県佐賀市)
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
  • [Presentation] レクリエーションビーチの砂浜におけるふん便指標細菌の存在実態と細菌叢解析2016

    • Author(s)
      寺西康太郎, 西山正晃, 鈴木祥広
    • Organizer
      第53回環境工学研究フォーラム講演集
    • Place of Presentation
      北九州国際会議場
    • Related Report
      2016 Annual Research Report
  • [Presentation] Relationships between temporal variability of genetic patterns and antibiotic-resistant profiles of fecal indicator bacteria, enterococci isolated from a human feces2015

    • Author(s)
      Nishiyama M, Shimauchi H, Suzuki Y
    • Organizer
      Water Environment Technology Conference, WET2015
    • Place of Presentation
      Nihon University(Tokyo, Japan)
    • Year and Date
      2015-08-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Classification of coliform group bacteria in municipal wastewater using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)2015

    • Author(s)
      Niina K, Iguchi A, Suzuki Y
    • Organizer
      Water Environment Technology Conference, WET2015
    • Place of Presentation
      Nihon University(Tokyo, Japan)
    • Year and Date
      2015-08-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 都市を流下する河川流域におけるバンコマイシン耐性腸球菌の拡散実態2015

    • Author(s)
      西山正晃, 鈴木祥広
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] レクリエーションビーチの砂浜におけるふん便汚染の実態調査2014

    • Author(s)
      西山正晃, 清水則年, 宇野瑞穂, 鈴木祥広
    • Organizer
      第17回日本水環境学会シンポジウム
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2014-09-08
    • Related Report
      2014 Research-status Report
  • [Remarks] 宮崎大学工学部社会環境システム工学科水環境研究室

    • URL

      http://www.suzuki-labo.com

    • Related Report
      2015 Research-status Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi