Effect of functional marine flavonoid on muscle atrophy

• Project/Area Number: 26350148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Nagasaki University
• Principal Investigator: HIRASAKA Katsuya 長崎大学, 水産・環境科学総合研究科(水産), 助教 (70432747)
• Co-Investigator(Kenkyū-buntansha): 二川 健 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (20263824)
• Co-Investigator(Renkei-kenkyūsha): TACHIBANA Katsuyasu 長崎大学, 水産・環境科学総合研究科(水産), 教授 (20171712)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 保健機能食品 / マリンフラボノイド / 加齢 / 筋萎縮 / ユビキチンリガーゼ

Outline of Final Research Achievements

In this study, we found flavonoid, morin in brown algae. Next, we examined the effect of dietary marine flavonoid containing morin, on the wasting of muscle. The wet weight and myofiber size of skeletal muscle in tumor-bearing mice, which is known to muscle atrophy model, significantly decreased, compared with that of vehicle-injected control mice. In contrast, the wet weight and myofiber size of skeletal muscle in tumor-bearing mice fed the marine flavonoid diet were nearly the same as those in vehicle-injected control mice. Moreover, treatment of marine flavonoid in C2C12 myotube inhibited myotube atrophy induced by tumor. These results suggest that dietary marine flavonoid suppresses muscle wasting in tumor-bearing mice. Thus, marine flavonoid may be efficacious in a therapeutic approach against the wasting of muscle.

Research Products

• [Journal Article] Dietary Supplementation with Isoflavones Prevents Muscle Wasting in Tumor-Bearing Mice (2016)
  • Author(s): Hirasaka K*, Saito S, Yamaguchi S, Miyazaki R, Wang Y, Haruna M, Taniyama S, Higashitani A, Terao J, Nikawa T, Tachibana K
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 62 Issue: 3 Pages: 178-184
  • DOI: 10.3177/jnsv.62.178
  • NAID: 130005253104
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Preventive effect of dietary quercetin on disuse muscle atrophy by targeting mitochondria in denervated mice (2016)
  • Author(s): Mukai R, Matsui N, Fujikura Y, Matsumoto N, Hou DX, Kanzaki N, Shibata H, Horikawa M, Iwasa K, Hirasaka K, Nikawa T, Terao J
  • Journal Title: J Nutr Biochem Volume: 31 Pages: 67-76
  • DOI: 10.1016/j.jnutbio.2016.02.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Capric Acid Up-Regulates UCP3 Expression without PDK4 Induction in Mouse C2C12 Myotubes (2016)
  • Author(s): Abe T, Hirasaka K, Kohno S, Tomida C, Haruna M, Uchida T, Ohno A, Oarada M, Teshima-Kondo S, Okumura Y, Choi I, Aoyama T, Terao J, Nikawa T*.
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 62 Issue: 1 Pages: 32-39
  • DOI: 10.3177/jnsv.62.32
  • NAID: 130005148603
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-obesity effects of enzymatically-digested alginate oligomer in mice model fed a high-fat-diet (2016)
  • Author(s): Nakazono S, Cho K, Isaka S, Abu R, Yokose T, Murata M, Ueno M, Tachibana K, Hirasaka K, Kim D, Oda T
  • Journal Title: Bioactive Carbohydrates and Dietary Fibre Volume: 7 Issue: 2 Pages: 1-8
  • DOI: 10.1016/j.bcdf.2016.02.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] UCP3 is associated with Hax-1 in mitochondria in the presence of calcium ion (2016)
  • Author(s): Hirasaka K, Mills EM, Haruna M, Bando A, Ikeda C, Abe T, Kohno S, Nowinski SM, Lago CU, Akagi K, Tochio H, Ohno A, Teshima-Kondo S, Okumura Y, Nikawa T
  • Journal Title: Biochem Biophys Res Commun Volume: 472 Issue: 1 Pages: 108-113
  • DOI: 10.1016/j.bbrc.2016.02.075
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin (2016)
  • Author(s): Ohno A, Ochi A, Maita N, Ueji T, Bando A, Nakao R, Hirasaka K, Abe T, Teshima-Kondo S, Nemoto H, Okumura Y, Higashibata A, Yano S, Tochio H, Nikawa T
  • Journal Title: Arch Biochem Biophys Volume: 594 Pages: 1-7
  • DOI: 10.1016/j.abb.2016.02.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel myogenic function residing in the 5’ non-coding region of Insulin receptor substrate-1 (Irs-1) transcript. (2015)
  • Author(s): Nagano H, Yamagishi N, Tomida C, Yano C, Aibara K, Kohno S, Abe T, Ohno A, Hirasaka K, Okumura Y, Mills EM, Nikawa T, Teshima-Kondo S.
  • Journal Title: BMC Cell Biol Volume: in press Issue: 1 Pages: 1-10
  • DOI: 10.1186/s12860-015-0054-8
  • NAID: 120006859993
  • Peer Reviewed / Open Access
• [Journal Article] The malignant progression effects of regorafenib in human colon cancer cells (2015)
  • Author(s): Tomida C, Aibara K, Yamagishi N, Yano C, Nagano H, Abe T, Ohno A, Hirasaka K, Nikawa T, Teshima-Kondo S
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 3.4 Pages: 195-198
  • DOI: 10.2152/jmi.62.195
  • NAID: 130005099146
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of dietary soy protein on skeletal muscle volume and strength in humans with various physical activities (2015)
  • Author(s): Hashimoto R, Sakai A, Murayama M, Ochi A, Abe T, Hirasaka K, Ohno A, Teshima-Kondo S, Yanagawa H, Yasui N, Inatsugi M, Doi D, Takeda M, Mukai R, Terao J, Nikawa T
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 3.4 Pages: 177-183
  • DOI: 10.2152/jmi.62.177
  • NAID: 130005099151
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access
• [Journal Article] Prevention of skeletal muscle atrophy in vitro using anti-ubiquitination oligopeptide carried by atelocollagen (2015)
  • Author(s): Kawai N, Hirasaka K, Maeda T, Haruna M, Shiota C, Ochi A, Abe T, Kohno S, Ohno A, Teshima-Kondo S Mori H, Tanaka E, Nikawa T
  • Journal Title: Biochim Biophys Acta Molecular Cell Research Volume: 1853 Issue: 5 Pages: 873-880
  • DOI: 10.1016/j.bbamcr.2015.01.024
  • Peer Reviewed / Open Access
• [Journal Article] N-Myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle. (2015)
  • Author(s): Ochi A, Abe T, Nakao R, Yamamoto Y, Kitahata K, Takagi M, Hirasaka K, Ohno A, Teshima-Kondo S, Taesik G, Choi I, Kawamura T, Nemoto H, Mukai R, Terao J, Nikawa T.
  • Journal Title: Arch Biochem Biophys Volume: 570 Pages: 23-31
  • DOI: 10.1016/j.abb.2015.02.006
  • Peer Reviewed
• [Journal Article] Chronic exposure of VEGF inhibitors promotes the malignant phenotype of colorectal cancer cells (2015)
  • Author(s): Tomida C, Yamagishi N, Aibara K, Yano C, Uchida T, Abe T, Ohno A, Hirasaka K, Nikawa T, Teshima-Kondo S.
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 1.2 Pages: 75-79
  • DOI: 10.2152/jmi.62.75
  • NAID: 130005129700
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access
• [Journal Article] Changes in the pericellular connective tissue and breaking strength of the three types of muscles of the cultured carp Cyprinus carpio during storage in ice. (2014)
  • Author(s): 4.Liang J, Miyazaki R, Zhao X, Hirasaka K, Taniyama S, Tachibana K.
  • Journal Title: Fisheries Science Volume: 80 Pages: 1083-1088
  • Peer Reviewed
• [Presentation] ユビキチンリガーゼを介した筋萎縮の分子メカニズムと機能性食品によるその予防法 (2016)
  • Author(s): 平坂勝也
  • Organizer: 第62回日本宇宙航空環境医学会大会 日本宇宙生物科学会第30回大会 合同大会
  • Place of Presentation: 愛知医科大学たちばなホール（愛知県長久手市）
  • Year and Date: 2016-10-13
• [Presentation] 微小重力環境下における筋萎縮の分子メカニズムと栄養素によるその予防法 (2016)
  • Author(s): 平坂勝也
  • Organizer: 日本農芸化学会2016年度西日本支部大会
  • Place of Presentation: 長崎大学文教キャンパス 環境科学部・教養教育講義棟（長崎県長崎市）
  • Year and Date: 2016-09-15
• [Presentation] ロコモティブ・メタボリックシンドローム予防のための栄養学的アプローチ (2016)
  • Author(s): 平坂勝也
  • Organizer: 第26回 体力・栄養・免疫学会大会
  • Place of Presentation: 長崎大学文教キャンパス文教スカイホール（長崎県長崎市）
  • Year and Date: 2016-08-27
  • Invited
• [Presentation] Dietary flavonoids prevent ubiquitin ligases-mediated muscle wasting in tumor-bearing mice (2016)
  • Author(s): Katsuya Hirasaka, Takeshi Nikawa, Yao Wang, Takayuki Uchida, Reiko Nakao Shigeto Taniyama, Katsuyasu Tachibana
  • Organizer: 「Molecular Mechanisms Modulating Skeletal Muscle Development and Homeostasis in Health and Disease」conference
  • Place of Presentation: Asilomar Conference Grounds, Pacific Grove, CA, USA
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] 食事性イソフラボン摂取はカヘキシーによる筋萎縮を予防する (2016)
  • Author(s): 髙木麻理奈、平坂勝也、齋藤志伸、宮﨑里帆、谷山茂人、橘勝康、二川健
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-13
• [Presentation] IGF-1シグナル経路の活性化を介した廃用性筋萎縮予防法 (2016)
  • Author(s): 平坂勝也、山田明徳、内田貴之、中尾玲子、二川健
  • Organizer: 第30回宇宙環境利用シンポジウム
  • Place of Presentation: 宇宙航空研究機構 宇宙科学研究所 相模原キャンパス（神奈川県相模原市）
  • Year and Date: 2016-01-19
• [Presentation] カヘキシーによる筋萎縮に対するフラボノイド類の効果 (2015)
  • Author(s): 平坂勝也、齋藤志伸、宮﨑里帆、谷山茂人、二川健、橘勝康
  • Organizer: 平成27年度 日本栄養・食糧学会九州・沖縄支部大会
  • Place of Presentation: 沖縄県市町村自治会館（沖縄県那覇市）
  • Year and Date: 2015-10-30
• [Presentation] Dietary flavonoid intake prevents muscle wasting in tumor-bearing mice (2015)
  • Author(s): Katsuya Hirasaka, Shinobu Saito, Saki Yamaguchi, Riho Miyazaki, Shigeto Taniyama, Takeshi Nikawa, Katsuyasu Tachibana
  • Organizer: 第69回日本栄養・食糧学会大会、12th Asian Congress of Nutrition
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] これからの食品機能研究をリードするための米国での分子栄養学に関する在外研究 (2015)
  • Author(s): 平坂 勝也
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都港区）
  • Year and Date: 2015-03-27 – 2015-03-31
  • Invited
• [Presentation] 廃用性筋萎縮に対する食品中フラボノイド類の阻害効果 (2014)
  • Author(s): 平坂勝也、春名真里江、安倍知紀、真板綾子、近藤茂忠、宮崎里帆、谷山茂人、橘勝康、二川健
  • Organizer: 日本宇宙生物科学会・第28回大会
  • Place of Presentation: 大阪府立大学・中百舌鳥キャンパス学術交流会館（大阪府堺市）
  • Year and Date: 2014-09-22 – 2014-09-23
• [Presentation] 廃用性筋萎縮予防に関する分子栄養学的研究 (2014)
  • Author(s): 平坂 勝也
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 札幌市教育文化会館大ホール(北海道札幌市）
  • Year and Date: 2014-05-30 – 2014-06-01
  • Invited
• [Remarks] 長崎大学水産科学領域 研究室紹介
  • URL: http://www.fe.nagasaki-u.ac.jp/intro/index.html
• [Remarks] 長崎大学教員個人業績
  • URL: http://research.jimu.nagasaki-u.ac.jp/IST?ISTActId=FINDJPDetail&ISTKidoKbn=&ISTErrorChkKbn=&ISTFormSetKbn=&ISTTokenChkKbn=&userId=100000917