|Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|Outline of Final Research Achievements
To capture molecular markers that are applicable to species identification of three Brassica species (B. rapa, B. napus, B. juncea), I screened variations in nucleotide sequences of chloroplast and nuclear DNA. Detection of DNA polymorphisms were analyzed by PCR-RFLPs (Restriction fragment length polymorphisms) and PCR-SSCP (Single strand conformation polymorphisms), but hypervariable loci that enables us to discriminate three Brassica species could not be captured.