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Quantitative evaluation of the change in action potential and axonal injury in brain neurons exposed to impulsive strain

Research Project

Project/Area Number 26350509
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Metropolitan University

Principal Investigator

Nakadate Hiromichi  首都大学東京, システムデザイン学部, 助教 (10514987)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords頭部外傷 / 軸索損傷 / ひずみ / ひずみ速度 / 軸索伸長方向制御 / Axonal swellings / Axonal bulb / Tauタンパク質 / 衝撃ひずみ / 神経幹細胞 / 軸索配向制御 / マイクロコンタクトプリンティング / 細胞パターニング / 細胞引張試験 / 神経軸索損傷 / 細胞外電位記録 / アミロイド前駆体タンパク質 / 軸索輸送障害 / びまん性軸索損傷
Outline of Final Research Achievements

Axonal injury is caused by sudden inertial loading to the head associated with rapid deformation of brain tissue, resulting in the stretching of neural axons. This study aims to clarify the relation between axonal stretch and the onset of axonal injury. To examine the influence of strain rate on the threshold for axonal injury, cultured neurons were subjected to uniaxial stretching. Axonal injury increased significantly more than strain rate of 30 /s with more than strain of 0.15 even though those formations did not depend on strain rates in cultures exposed to a strain of 0.10. To examine the influence of the strain along an axon on axonal injury axonal injury was measured at every axonal angle in the stretching direction. The axons that were parallel to stretching direction were injured the most. We proposed an experimental model that subjected an axon to more accurate strain for the measurement of action potential in single neuron before and after stretching.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(28 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (25 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Journal Article] Uniaxial stretch-induced axonal injury thresholds for axonal dysfunction and disruption and strain rate effects on thresholds for mouse neuronal stem cells2017

    • Author(s)
      Kurtoglu, E., Nakadate, H., Kikuta, K., Aomura, S. and Kakuta, A.
    • Journal Title

      JBSE

      Volume: 12 Issue: 1 Pages: 16-00598-16-00598

    • DOI

      10.1299/jbse.16-00598

    • NAID

      130005520034

    • ISSN
      1880-9863
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Repetitive stretching enhances the formation of neurite swellings in cultured neuronal cells2016

    • Author(s)
      Nakadate, H., Kurtoglu, E., Shirasaki, S. and Aomura, S.
    • Journal Title

      Integrative Molecular Medicine

      Volume: 3 Pages: 723-728

    • DOI

      10.15761/imm.1000231

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] <i>In vitro</i> uniaxial stretch model for evaluating the effect of strain along axon on damage to neurons2014

    • Author(s)
      Nakadate, H., Fukumura, Y., Kaneko, Y., Kakuta, A., Furukawa, H. and Aomura, S.
    • Journal Title

      JBSE

      Volume: Vol.9, No.3 Issue: 3 Pages: 14-00136-14-00136

    • DOI

      10.1299/jbse.14-00136

    • NAID

      130004678608

    • ISSN
      1880-9863
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of axonal stretching device for in vitro repetitive mild traumatic brain injury model2017

    • Author(s)
      Shirasaki, S., Nakadate, H., Aomura, S. and Kakuta, A.
    • Organizer
      XXVI Congress of the International Society of Biomechanics
    • Place of Presentation
      Brisbane (Australia)
    • Year and Date
      2017-07-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contribution of repetitive stretching to neurite injury in cortex primary neuronal cells2017

    • Author(s)
      Shirasaki, S., Nakadate, H. and Aomura, S.
    • Organizer
      2017 Summer Biomechanics, Bioengineering and Biotransport Conference
    • Place of Presentation
      Tucson (USA)
    • Year and Date
      2017-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 頭部外傷における細胞レベルの変化2017

    • Author(s)
      中楯浩康,青村茂,角田陽
    • Organizer
      第15回日本機械学会傷害バイオメカニクス研究会
    • Place of Presentation
      東京都市大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2017-02-24
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 繰り返し引張ひずみを受ける神経細胞の経時的観察2017

    • Author(s)
      白崎祥多,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 衝撃引張による神経軸索損傷のひずみ速度依存性2017

    • Author(s)
      鶴見明冴美,Kurtoglu Evrim,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経軸索の方向制御技術を応用した細胞引張実験と損傷評価2017

    • Author(s)
      古川英典,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脳神経細胞のひずみ耐性評価2016

    • Author(s)
      中楯浩康,Kurtoglu Evrim,青村茂,角田陽,松井靖浩
    • Organizer
      自動車技術会2016年秋季大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 培養神経細胞の衝撃耐性評価のための一軸引張装置の開発2016

    • Author(s)
      中楯浩康,古川英典,佐藤尚樹,青村茂,角田陽
    • Organizer
      第43回日本臨床バイオメカニクス学会
    • Place of Presentation
      北海道立道民活動センターかでる2・7(北海道札幌市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脳神経細胞の衝撃ひずみ耐性評価2016

    • Author(s)
      中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会2016年度年次大会
    • Place of Presentation
      九州大学伊都キャンパス(福岡県福岡市)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 衝撃引張ひずみによる培養神経細胞の軸索損傷評価2016

    • Author(s)
      中楯浩康,青村茂,Kurtoglu Evrim,古川英典,佐藤尚樹
    • Organizer
      Dynamics and Design Conference 2016
    • Place of Presentation
      山口大学常盤キャンパス(山口県宇部市)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脳神経細胞における耐性値の開発2016

    • Author(s)
      中楯浩康,青村茂,角田陽
    • Organizer
      第52回日本交通科学学会学術講演会
    • Place of Presentation
      東京都立産業技術高等専門学校荒川キャンパス(東京都荒川区)
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 繰り返し低ひずみが与える神経細胞の損傷評価2016

    • Author(s)
      白崎祥多,青村茂,中楯浩康
    • Organizer
      日本機械学会関東学生会第55回学生員卒業研究発表講演会
    • Place of Presentation
      東京工業大学 大岡山キャンパス
    • Year and Date
      2016-03-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 外傷性脳損傷研究のための衝撃試験装置の開発と脳細胞の耐性評価2016

    • Author(s)
      中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会バイオエンジニアリング部門
    • Place of Presentation
      東京都市大学 世田谷キャンパス
    • Year and Date
      2016-02-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 衝撃引張ひずみを受ける培養神経細胞の耐性評価2016

    • Author(s)
      Kurtoglu Evrim,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学 大岡山キャンパス
    • Year and Date
      2016-01-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 脳神経細胞の耐衝撃回転加速度の評価のための実験装置の設計・開発2016

    • Author(s)
      佐藤尚樹,Kurtoglu, Evrim,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2016-01-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 伸長方向制御した神経軸索の衝撃引張ひずみ耐性評価2016

    • Author(s)
      古川英典,Kurtoglu, Evrim,中楯浩康,青村茂,角田陽
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学 大岡山キャンパス
    • Year and Date
      2016-01-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 外傷性脳損傷研究のための衝撃試験装置の開発と脳組織・細胞の耐性評価2016

    • Author(s)
      中楯浩康,青村茂,角田陽
    • Organizer
      日本生体医工学会専門別研究会 第157回バイオメカニクス研究会
    • Place of Presentation
      首都大学東京 日野キャンパス
    • Year and Date
      2016-01-08
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Injury threshold for axonal transport by observation of β-amyloid precursor protein in cultured rat brain neurons exposed to impulsive strain2015

    • Author(s)
      Hiromichi NAKADATE, Kazuhiro KIKUTA, Evrim KURTOGLU, Shigeru AOMURA, Akira KAKUTA, Caroline DECK, Remy WILLINGER
    • Organizer
      The 8th Asian-Pacific Conference on Biomechanics
    • Place of Presentation
      Hokkaido University, Japan
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evaluation of effect of impulsive strain and growth direction control of cultured brain neuronal cells2015

    • Author(s)
      Hidenori Furukawa, Akira KAKUTA, Hiromichi NAKADATE, Shigeru AOMURA
    • Organizer
      The 8th Asian-Pacific Conference on Biomechanics
    • Place of Presentation
      Hokkaido University, Japan
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 衝撃ひずみを負荷した培養脳神経細胞における軸索輸送の損傷閾値2015

    • Author(s)
      中楯浩康,佐藤尚樹,青村茂,角田陽
    • Organizer
      日本機械学会2015年度年次大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 衝撃ひずみが脳神経細胞の情報伝達に与える影響2015

    • Author(s)
      菊田和紘, 中楯浩康, 青村茂, 角田陽
    • Organizer
      日本機械学会第27回バイオエンジニアリング講演会
    • Place of Presentation
      新潟
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 微細凹凸溝によるPC12細胞の神経突起の伸長方向制御2014

    • Author(s)
      古川英典, 角田陽, 中楯浩康, 青村茂
    • Organizer
      2014年度精密工学会秋季大会学術講演会
    • Place of Presentation
      鳥取
    • Year and Date
      2014-09-16 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] β-APP観察による脳神経軸索の衝撃耐性評価2014

    • Author(s)
      中楯浩康, 菊田和紘, 鶴見明冴美, 青村茂, 角田陽
    • Organizer
      日本機械学会2014年度年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Diagnosis of Axonal Injury by observing the leaked β-APP2014

    • Author(s)
      Aomura, S. and Nakadate, H.
    • Organizer
      The 4th Japan-Switzerland Workshop on Biomechanics
    • Place of Presentation
      Shima, Mie, Japan
    • Year and Date
      2014-09-01 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Evaluation of Stretch-injured Axon in Brain Neuronal Cell by Observation of β-Amyloid Precursor Protein2014

    • Author(s)
      Nakadate, H., Kaneko, Y., Kikuta, K., Aomura, S. and Kakuta, A.
    • Organizer
      7th World Congress of Biomechanics
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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