Quantitative evaluation of the change in action potential and axonal injury in brain neurons exposed to impulsive strain
| Project/Area Number |
26350509
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 頭部外傷 / 軸索損傷 / ひずみ / ひずみ速度 / 軸索伸長方向制御 / Axonal swellings / Axonal bulb / Tauタンパク質 / 衝撃ひずみ / 神経幹細胞 / 軸索配向制御 / マイクロコンタクトプリンティング / 細胞パターニング / 細胞引張試験 / 神経軸索損傷 / 細胞外電位記録 / アミロイド前駆体タンパク質 / 軸索輸送障害 / びまん性軸索損傷 |
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| Outline of Final Research Achievements |
Axonal injury is caused by sudden inertial loading to the head associated with rapid deformation of brain tissue, resulting in the stretching of neural axons. This study aims to clarify the relation between axonal stretch and the onset of axonal injury. To examine the influence of strain rate on the threshold for axonal injury, cultured neurons were subjected to uniaxial stretching. Axonal injury increased significantly more than strain rate of 30 /s with more than strain of 0.15 even though those formations did not depend on strain rates in cultures exposed to a strain of 0.10. To examine the influence of the strain along an axon on axonal injury axonal injury was measured at every axonal angle in the stretching direction. The axons that were parallel to stretching direction were injured the most. We proposed an experimental model that subjected an axon to more accurate strain for the measurement of action potential in single neuron before and after stretching.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] <i>In vitro</i> uniaxial stretch model for evaluating the effect of strain along axon on damage to neurons2014
Author(s)
Nakadate, H., Fukumura, Y., Kaneko, Y., Kakuta, A., Furukawa, H. and Aomura, S.
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Journal Title
JBSE
Volume: Vol.9, No.3
Issue: 3
Pages: 14-00136-14-00136
DOI
NAID
ISSN
1880-9863
Related Report
Peer Reviewed / Open Access
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[Presentation] 頭部外傷における細胞レベルの変化2017
Author(s)
中楯浩康，青村茂，角田陽
Organizer
第15回日本機械学会傷害バイオメカニクス研究会
Place of Presentation
東京都市大学世田谷キャンパス（東京都世田谷区）
Year and Date
2017-02-24
Related Report
Invited
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[Presentation] Evaluation of Stretch-injured Axon in Brain Neuronal Cell by Observation of β-Amyloid Precursor Protein2014
Author(s)
Nakadate, H., Kaneko, Y., Kikuta, K., Aomura, S. and Kakuta, A.
Organizer
7th World Congress of Biomechanics
Place of Presentation
Boston, MA, USA
Year and Date
2014-07-06 – 2014-07-11
Related Report
