Development of high contrast fluorescence microscopy using enhanced fluorescence of an optical interference mirror slide
Project/Area Number |
26350518
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | Tokyo University of Technology |
Principal Investigator |
Akimoto Takuo 東京工科大学, 応用生物学部, 教授 (90367194)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | バイオセンサー / 蛍光増強 / 蛍光顕微鏡 |
Outline of Final Research Achievements |
An enhanced fluorescence can be observed with an optical interference mirror slide consisting of a metal and a dielectric layer. However, a general fluorescence microscopy does not provide the enhanced fluorescence because of the polarization dependence of the enhanced fluorescence. To obtain the enhanced fluorescence, we developed a fluorescence microscope equipped with a concentric circular polarized excitation. As a result, 42-fold enhanced fluorescence was achieved by the microscope with the concentric circular polarized excitation.
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Report
(4 results)
Research Products
(8 results)