Effects of the mechanical interactions between collagen molecules on the mechanical adaptation of biological tissues

• Project/Area Number: 26350519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Ritsumeikan University
• Principal Investigator: YAMAMOTO Noritaka 立命館大学, 理工学部, 教授 (40210546)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: バイオメカニクス / 生体分子 / 細胞・組織 / 再生医学

Outline of Final Research Achievements

Collagen fibrils were reconstituted in vitro from collagen solution. The interaction between the reconstituted collagen fibrils and chondroitin sulfate B was analyzed using a quartz crystal microbalance (QCM). The obtained results indicate that chondroitin sulfate B is effective on the bonding of collagen fibrils. The tensile test and microscopic observation of reconstituted collagen fibrils were performed. Their tensile properties and microstructures were equal to those of native collagen fibrils of tendons.

Research Products

• [Journal Article] 再構成コラーゲン原線維の力学的性質に及ぼすテロペプチドの影響 (2017)
  • Author(s): 山本憲隆，松田侑鷹
  • Journal Title: 日本機械学会第29回バイオエンジニアリング講演会講演論文集 Volume: 16-76
  • NAID: 130007126681
  • Acknowledgement Compliant
• [Journal Article] Effects of chondroitin sulfate B on the reconstitution of collagen fibrils (2017)
  • Author(s): 山本憲隆，佐々木健
  • Journal Title: Transactions of the JSME (in Japanese) Volume: 83 Issue: 848 Pages: 16-00343-16-00343
  • DOI: 10.1299/transjsme.16-00343
  • NAID: 130005612183
  • ISSN: 2187-9761
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 再構成コラーゲン原線維の微細構造と力学的性質 (2016)
  • Author(s): 山本憲隆，河田耀平
  • Journal Title: 日本機械学会第28回バイオエンジニアリング講演会論文集 Volume: 15-69
  • Acknowledgement Compliant
• [Journal Article] コラーゲン原線維の再構成に及ぼす溶液流れの影響 (2015)
  • Author(s): 長坂晃希，山本憲隆
  • Journal Title: 日本機械学会第26回バイオフロンティア講演会論文集 Volume: 15-40 Pages: 67-68
  • Acknowledgement Compliant
• [Journal Article] Mechanical Properties of Self-Assembled Collagen Fibrils (2014)
  • Author(s): Noritaka Yamamoto, Meguru Matsuda
  • Journal Title: Proceedings of the Fourth Japan-Switzerland Workshop on Biomechanics Volume: - Pages: 130-130
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 再構成コラーゲン原線維とコンドロイチン硫酸Bの相互作用の解析 (2014)
  • Author(s): 佐々木健, 山本憲隆
  • Journal Title: 日本機械学会第25回バイオフロンティア講演会講演論文集 Volume: - Pages: 29-30
  • Acknowledgement Compliant
• [Presentation] 再構成コラーゲン原線維の力学的性質に及ぼすテロペプチドの影響 (2017)
  • Author(s): 山本憲隆，松田侑鷹
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウインクあいち（愛知県名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 再構成コラーゲン原線維の微細構造と力学的性質 (2016)
  • Author(s): 山本憲隆，河田耀平
  • Organizer: 日本機械学会第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-01-10
• [Presentation] コラーゲン原線維の再構成に及ぼす溶液流れの影響 (2015)
  • Author(s): 長坂晃希，山本憲隆
  • Organizer: 日本機械学会第26回バイオフロンティア講演会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2015-10-02
• [Presentation] 再構成コラーゲン原線維とコンドロイチン硫酸Bの相互作用の解析 (2014)
  • Author(s): 佐々木健, 山本憲隆
  • Organizer: 日本機械学会第25回バイオフロンティア講演会
  • Place of Presentation: とりぎん文化会館（鳥取県）
  • Year and Date: 2014-10-03
• [Presentation] Mechanical Properties of Self-Assembled Collagen Fibrils (2014)
  • Author(s): Noritaka Yamamoto, Meguru Matsuda
  • Organizer: The Fourth Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: 志摩観光ホテル(三重県）
  • Year and Date: 2014-09-04
  • Invited