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Development of Early Diagnosis System for Alzheimer's Disease by Electrochemical Detection of Amyloid beta peptide

Research Project

Project/Area Number 26350552
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical systems
Research InstitutionKonan University

Principal Investigator

FUJII Satoshi  甲南大学, フロンティアサイエンス学部, 教授 (80271518)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsアルツハイマー病 / アミロイドβ / 電気化学センサー / 早期診断 / バイオセンサー / ペプチド / 電気化学 / 診断システム / 認知症 / 修飾電極 / 測定法 / アルツハイマー型認知症 / プロテインチップ
Outline of Final Research Achievements

We have developed an electrochemical sensor that aspires for early diagnosis of Alzheimer’s disease by quantifying plasma amyloid-beta peptide. Amyloid-beta (Abeta) fibrillization promoting peptide, AFPP, which we have developed, was immobilized on a gold electrode, and then Abeta was added. After accumulation of Abeta on AFPP, cupper ion was added and electrochemical measurements were carried out. By using this procedure, we succeeded in measuring Abeta with the limit of detection, 5 nM. To improve the sensitivity, we made a golod nanoparticle modified electrode. Although the sensitivity was improved, the physical strength of the modified electrode became worth. Therefore, there is a problem with reusability after initialization by washing. Currently the sensitivity of our method does not reach the plasma Abeta level. Further investigations are now under way.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Site-specific control of silica mineralization on DNA using a designed peptide2016

    • Author(s)
      Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, and Kenji Usui
    • Journal Title

      Chemical Communcations

      Volume: 52 Issue: 21 Pages: 4010-4016

    • DOI

      10.1039/c5cc07870a

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrochemical quantification of the Alzheimer’s disease amyloid-β (1-40) using amyloid-β fibrillization promoting peptide2015

    • Author(s)
      Satoshi Fujii, Daiki Sono, Kaoru Matsubara, and Hitoshi Abe
    • Journal Title

      Sensing and Bio-Sensing Research

      Volume: 6 Pages: 7-12

    • DOI

      10.1016/j.sbsr.2015.09.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-catalyzed Oxidative Reactions in the Presence of Betaine-type Metabolite Analogs2015

    • Author(s)
      Kotomi Takagi, Yasuhiro Kashima, Satoshif Fujii, and Kazuya Koumoto
    • Journal Title

      Bulletin of Chemical Society of Japan

      Volume: 88 Issue: 8 Pages: 1074-1082

    • DOI

      10.1246/bcsj.20150050

    • NAID

      130005092061

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Facile Quatification of Alzheimer’s Disease Amyloid-β Based On Aggregation-Induced Emission2015

    • Author(s)
      K. Kawamura, R. Nakayama, A. Matsumoto, S. Fujii, and T. Murashima
    • Journal Title

      Journal of Pharmaceutical and Medicinal Research

      Volume: 1 Pages: 27-30

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs2014

    • Author(s)
      Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, and Kazuya Koumoto
    • Journal Title

      Tetrahedron

      Volume: 70 Pages: 5895-5903

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 金ナノ粒子修飾電極を用いたアミロイドβ測定法の高感度化2017

    • Author(s)
      國光祐希、園大樹、中村暢文、藤井敏司
    • Organizer
      電気化学会第84回大会
    • Place of Presentation
      首都大学東京(東京都八王子市)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 金ナノ粒子修飾電極を用いたアミロイドβ定量法の高感度化2016

    • Author(s)
      國光祐希、中村暢文、藤井敏司
    • Organizer
      第10回バイオ関連化学シンポジウム2016
    • Place of Presentation
      石川県立音楽堂・もてなしドーム(石川県金沢市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] アルツハイマー型認知症の早期リスク評価を可能にするアミロイドβ測定法の開発2016

    • Author(s)
      藤井敏司
    • Organizer
      第15回神戸ポートアイランド創薬フォーラム
    • Place of Presentation
      甲南大学(兵庫県神戸市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] The development of diagnosis tools for Alzheimer’s diseases based on AIE phenomenon2015

    • Author(s)
      Takashi Murashima, Koji Kawamura, Runa Nakayama, Ai Matsumoto, and Satoshi Fujii
    • Organizer
      Pacifichem
    • Place of Presentation
      Hawaii、アメリカ合衆国
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 凝集誘起発光(AIE)色素標識蛍光プローブを用いた生体分子検出法の開発2014

    • Author(s)
      河村浩司、松本亜衣、中山瑠奈、藤井敏司、村嶋貴之
    • Organizer
      第8回バイオ合同シンポジウム
    • Place of Presentation
      岡山大学(岡山県・岡山市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 甲南大学フロンティアサイエンス学部生物無機化学研究室

    • URL

      http://fujiilab.my.coocan.jp/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 甲南大学研究者データベース

    • URL

      http://researchers.adm.konan-u.ac.jp/html/230_ja.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 甲南大学フロンティアサイエンス学部生物無機化学研究室ホームページ

    • URL

      http://homepage2.nifty.com/gon-ta/index.html

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] アミロイドβペプチドを電気化学的に測定するためのバイオセンサ2015

    • Inventor(s)
      藤井敏司
    • Industrial Property Rights Holder
      学校法人甲南学園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-060724
    • Filing Date
      2015-03-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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