immobilization of skeletal muscle increases mTORC1 signaling and LAT1 expression both at stretched and shortened positions but only increases protein synthesis at stretched position
| Project/Area Number |
26350823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Shigakkan University |
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Principal Investigator |
Murakami Taro 至学館大学, 健康科学部, 教授 (10252305)
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| Co-Investigator(Kenkyū-buntansha) |
恒川 春香 至学館大学, 健康科学部, 助手 (60723822)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アミノ酸輸送体 / mTORC1 / SUnSET / ロイシン / ギプス固定 / 筋肉の萎縮 / ロイシン輸送体 / 4E-BP1 / S6K1 / 筋肉 / 除神経 / 筋萎縮 |
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| Outline of Final Research Achievements |
We investigate whether a decrease in amino acid requirement in the muscle by immobilization with casting would inactivate the mammalian target of rapamycine complex 1 (mTORC1) signaling and decrease the leucine transporter (LAT1) expression. Both the mTORC1 signaling and the expression of LAT1 are activated in both immobilized muscles at shortened and stretched positions, even the extents are larger in the latter. However, muscle protein synthesis at fasted-state is only increased in a stretched muscle but not in a shortened muscle. Branched chain amino acids administration increases muscle protein synthesis in both shortened and stretched muscles.These findings suggest that the activation of mTORC1 signaling and induction of leucine transporter in the immobilized muscle at shortened position could not contribute to the protein synthesis. A decrease in atrophy in immobilized muscle at stretched position would due to be an increase in protein synthesis during preabsorptive state.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.2017
Author(s)
Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y.
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Journal Title
Sci Rep.
Volume: 7
Issue: 1
Pages: 39825-39825
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 運動選手とサプリメント2016
Author(s)
村上太郎
Organizer
日本未病システム学会栄養部会研究大会
Place of Presentation
愛知みずほ大学（愛知県・名古屋市）
Year and Date
2016-07-30
Related Report
Invited
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