The comprehensive analysis of target molecules of HSPBs in muscle exercise by the BirA labeling method.
| Project/Area Number |
26350889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Kobe University |
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Principal Investigator |
Shuji Ueda 神戸大学, 農学研究科, 助教 (50379400)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒートショック蛋白質 / RhoA / 運動 / 筋肉 / BirA / 国際情報交換 |
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| Outline of Final Research Achievements |
In this study, we succeeded in carrying out the comprehensive analysis of the binding protein of low molecular weight heat shock protein HSPB1 by the BirA enzyme-labeling method. Moreover, we revealed the involvement of RhoA-SLK signal in the enhancement of C2C12 myotube by IGF-I. In the future, we plan to develop BirA enzyme-labeling method into a quantitative experimental method to measure the molecular chaperone activity of HSPBs, and apply this methods as a research tool for screening functional ingredients to prevent muscular atrophy in the elderly.
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Report
(3 results)
Research Products
(9 results)
