| Project/Area Number |
26350892
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Okayama University |
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Principal Investigator |
Momota Ryusuke 岡山大学, 医歯薬学総合研究科, 助教 (80263557)
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| Co-Investigator(Kenkyū-buntansha) |
大塚 愛二 岡山大学, 医歯薬学総合研究科, 教授 (50168986)
内藤 一郎 岡山大学, 医学部, 客員研究員 (60362993)
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| Co-Investigator(Renkei-kenkyūsha) |
OOHASHI Toshitaka 岡山大学, 医歯薬学総合研究科, 教授 (50194262)
YONEZAWA Tomoko 岡山大学, 医歯薬学総合研究科, 助教 (30304299)
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| Research Collaborator |
KOMIYAMA Takaaki
NARASAKI Masahiro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | XV/XVIII型コラーゲン / ミトコンドリア / 細胞骨格 / 診断法 / 基底膜 / コラーゲン / ショウジョウバエ / アンギオテンシンII受容体1型 / チュブリン / アクチン / 細胞内骨格 |
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| Outline of Final Research Achievements |
To identify the molecules which would compose "the tran-plasma membrane molecular machinery" of collagen types XV/XVIII and mitochondria, we have screened thousands of genes expressed in muscular tissues and identified possible candidate G-protein coupled receptors. In addition, our histological analyses on collagen types XV/XVIII mutant animals revealed abnormal structures of cytoskeletons, which would potentially compromise the mitochondrial and cellular functions. Moreover, we had successfully optimized the immunohistochemical conditions for the anti-collagen XV/XVIII monoclonal antibodies, which enabled us to use variously processed tissue samples to diagnose human diseases caused by defective collagen XV/XVIII.
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