Search for the drug-responsive aminoproduct during the perinatal period and analysis of its reactive mechanism.
Project/Area Number |
26350957
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biomolecular chemistry
|
Research Institution | University of Tsukuba |
Principal Investigator |
KAKO Koichiro 筑波大学, 生命環境系, 講師 (60311594)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | アミノ基 / アミノプロダクト / 妊娠高血圧 / PAHマウス / MALDI-QIT-TOF/MS / LC-MS/MS / tele-メチルヒスタミン / モノアミンオキシダーゼ / ヒドララジン / トラニルシプロミン / リジン特異的デメチラーゼ 1 / フラビンアデニンジヌクレオチド / メチル化アルギニン / PRMT / HPLC / UPLC |
Outline of Final Research Achievements |
We identified ethanolamine (Etn) and tele-methylhistamine (tMH), as aminoproducts altering in embryonic tissue and maternal blood in response to antihypertensive drugs administered to pregnancy-associated hypertensive mouse. The high level of Etn in late-gestation embryo was responsive to the treatment with both the vasoconstriction suppressant (Hdz) and the angiotensin receptor antagonist (ARB), and ameliorated to normal level.On the other hand, the increase of maternal tMH level depended on the Hdz, not ARB. Since Hdz was shown to abrogate the enzymatic activity of monoamine oxidase B, a metabolic enzyme for tMH, as dose dependent manner in vitro, it is speculated that the inhibition of monoamine oxidase B by Hdz led to the increase of maternal tMH level.
|
Report
(5 results)
Research Products
(41 results)
-
-
-
-
-
[Journal Article] Hydralazine is involved in tele-methylhistamine metabolism by inhibiting monoamine oxidase B in pregnancy-associated hypertensive mice.2017
Author(s)
Kawasaki, S., Kako, K., Nagashima, Y., Kanou, A., Ishida, J., and Fukamizu, A.
-
Journal Title
Journal of Biochemistry
Volume: 161
Pages: 155-158
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] Pterosin B has multiple targets in gluconeogenic programs, including coenzyme Q in RORα-SRC2 signaling.2016
Author(s)
Itoh, Y., Fuchino, H., Sanosaka, M., Kako, K., Hada, K., Fukamizu, A., Takemori, H., Kawahara, N.
-
Journal Title
Biochem. Biophys. Res. Commun.
Volume: 473
Issue: 2
Pages: 415-420
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Possible involvement of downregulation of the apelin-APJ system in doxorubicin-induced cardiotoxicity2015
Author(s)
Hamada, J., Baasanjav, A., Ono, N., Murata, K., Kako, K., Ishida, J., Fukamizu, A.
-
Journal Title
American Journal of Physiology-Heart and circulatory physiology
Volume: 印刷中
Issue: 8
Pages: 931-941
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.2015
Author(s)
Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
-
Journal Title
Sci. Adv.
Volume: 1
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-