Activation mechanism of the cellular Factor XIII for nerve regeneration
Project/Area Number |
26350958
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biomolecular chemistry
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Research Institution | Kanazawa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
北村 敬一郎 金沢大学, 保健学系, 教授 (80283117)
郡山 恵樹 鈴鹿医療科学大学, 薬学部, 准教授 (70397199)
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Research Collaborator |
NODA Masaharu 基礎生物学研究所, 教授 (60172798)
SHINTANI Takafumi 基礎生物学研究所, 准教授 (10312208)
KATO Satoru 金沢大学, 健康増進科学センター, 研究協力員 (10019614)
HITOMI Kiyotaka 名古屋大学, 大学院創薬科学研究科, 教授 (00202276)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | Factor XIII-A / CNS regeneration / optic nerve / retina / cellular Factor XIII / zebrafish / activation peptide / repair / cellular FXIII / heat shock factor / transglutaminase / regeneration / neurite outgrowth / optioc nerve / activation mechanism / neurite sprouting |
Outline of Final Research Achievements |
Factor XIII-A (FXIII-A), also known as cellular transglutaminase, plays important roles in mediating cross-linking reactions in various tissues. Previous research has shown that FXIII-A was immediately upregulated in the fish retina and optic nerve after nerve injury. However, the activation mechanism of the FXIII-A remains unclear. Here, we investigated the activation mechanism of the FXIII-A using zebrafish CNS regeneration system. Thrombin mRNA was undetectable in zebrafish optic nerve and retina both before and after optic nerve injury. Sequence analysis of FXIII-A 5'-RACE products showed that most of clones derived from intact retina showed full sequence of FXIII-A, however, many clones derived from injured retina showed short sequences of FXIII-A which lacked exon 1-2 region. Therefore, unlike plasma FXIII-A, activation of FXIII-A in injured retina and optic nerve does not need the cleavage by thrombin, may occur the directly production of activated FXIII-A protein.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales.2017
Author(s)
Kitamura K, Andoh T, Okesaku W, Tazaki Y, Ogai K, Sugitani K, Kobayashi I, Suzuki N, Chen W, Ikegame M, Hattori A
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Journal Title
Comparative Biochemistry and Physiology, Part A
Volume: 203
Pages: 152-158
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A possible role of neuroglobin in the retina after optic nerve injury: a comparative study of zebrafish and mouse retina.2016
Author(s)
Sugitani, K., Koriyama, Y., Ogai, K., Wakasugi, K., and Kato, S.
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Journal Title
Adv. Exp. Med. Res.
Volume: 854
Pages: 671-675
DOI
NAID
ISBN
9783319171203, 9783319171210
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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