Search for antiviral molecules from coral reef organisms

• Project/Area Number: 26350973
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: University of the Ryukyus
• Principal Investigator: TANAKA Junichi 琉球大学, 理学部, 教授 (20163529)
• Research Collaborator: NODA Naohiro 産業技術総合研究所, 主任研究員 (70415727) ; SEKIGUCHI Yuji 産業技術総合研究所, 主任研究員 (20313570) ; TANI Hidenori 産業技術総合研究所, 研究員 (10635329) ; MORIISHI Kohji 山梨大学, 大学院・総合研究部, 教授 (90260273) ; YAMASHITA Atsuya 山梨大学, 大学院・総合研究部, 助教 (00334871) ; TSUNEDA Satoshi 早稲田大学, 理工学術院, 教授 (30281645) ; AKIMITSU Nobuyoshi 東京大学, アイソトープ総合センター, 教授 (40294962) ; TSUBUKI Masayoshi 星薬科大学, 薬学部, 教授 (90163865) ; FUJITA Yoshihisa 沖縄県立芸術大学, 准教授 (20771463) ; HIGASHI Masahiro 琉球大学, 理学部, 助教 (20611479)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: HCV / HBV / NS3 helicase / core promoter / サンゴ礁生物 / メロセスキテルペン / HCV NS3 helicase / PBDE / merosesquiterpene / ビフェニルエーテル

Outline of Final Research Achievements

We constructed a library of extracts from coral reef organisms and a small library of marine natural products. They were screened for antiviral assays: HCV NS3 helicase, HCV replicon, and HBV core promoter. Some of hit extracts were separated and isolated compounds were characterized mainly by spectroscopic methods. After identifying hit molecules, we searched similar molecules and prepared derivatives by chemical transformations. The isolated compounds and derivatives were subjected for assays to see structure-activity relationship. We reported some of the results as papers or presentations in scientific meetings, though some of the contents are still under investigation.

Research Products

• [Journal Article] Four Aromatic Sulfates with an Inhibitory Effect against HCV NS3 Helicase from the Crinoid Alloeocomatella polycladia (2017)
  • Author(s): Idam Hermawan, Atsushi Furuta, Masahiro Higashi, Yoshihisa Fujita, Nobuyoshi Akimitsu, Atsuya Yamashita, Kohji Moriishi, Satoshi Tsuneda, Hidenori Tani, Masamichi Nakakoshi, Masayoshi Tsubuki, Yuji Sekiguchi, Naohiro Noda, Junichi Tanaka
  • Journal Title: Mar Drugs Volume: 15 Pages: 117-117
  • DOI: 10.3390/md15040117
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Four Cytotoxic Spongian Diterpenes from the Sponge <i>Dysidea</i> cf. <i>arenaria</i> (2016)
  • Author(s): M. Shingaki, T. Wauke, P. Ahmadi, and J. Tanaka
  • Journal Title: CHEMICAL & PHARMACEUTICAL BULLETIN Volume: 64 Issue: 3 Pages: 272-275
  • DOI: 10.1248/cpb.c15-00726
  • NAID: 130005131595
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of antiviral agents targeting hepatitis B virus promoter from extracts of Indonesian marine organisms by a novel cell-based screening assay. (2015)
  • Author(s): Yamashita A, Fujimoto Y, Tamaki M, Setiawan A, Tanaka T, Okuyama-Dobashi K, Kasai H, Watashi K, Wakita T, Toyama M, Baba M, de Voogd NJ, Maekawa S, Enomoto N, Tanaka J, Moriishi K
  • Journal Title: Mar Drugs Volume: 13 Pages: 6759-6773
  • DOI: 10.3390/md13116759
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of hydroxyanthraquinones as novel inhibitors of hepatitis C virus helicase (2015)
  • Author(s): Atsushi Furuta, Masayoshi Tsubuki, Miduki Endoh, Tatsuki Miyamoto, Junichi Tanaka, Kazi Abdus Salam, Nobuyoshi Akimitsu, Hidenori Tani, Atsuya Yamashita, Kohji Moriishi, Masamichi Nakakoshi, Yuji Sekiguchi, Satoshi Tsuneda, Naohiro Noda
  • Journal Title: Int H Mol Sci Volume: 16 Pages: 18439-18453
  • DOI: 10.3390/ijms160818439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Search for antiviral molecules from coral reef organisms and biodiversity aspects of secondary metabolites (2017)
  • Author(s): Junichi Tanaka
  • Organizer: The 6th Symposium - Riken Max Planck Institute Joint Research Center
  • Place of Presentation: Pacific Hotel, Naha, Okinawa
  • Year and Date: 2017-04-24
  • Int'l Joint Research / Invited
• [Presentation] A new cyclic imine and portimine from an Okinawan dinoflagellate (2016)
  • Author(s): I. Hermawan, T. Fujiwara, A. Kanamoto, T. Haruyama, N. Kobayashi, J. Tanaka
  • Organizer: International Symposium on Natural Products for the Future 2016
  • Place of Presentation: Tokushima Bunri University, Tokushima
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] カイメン由来化合物metachromin A及びその類縁化合物の抗HBV活性の検討 (2015)
  • Author(s): 山下篤哉、田中智久、葛西宏威、渡士幸一、脇田隆字、外山正明、馬場昌範、前川伸哉、榎本信幸、玉城麻友美、田中 淳一、森石恆司
  • Organizer: 第25回抗ウイルス療法学会総会
  • Place of Presentation: リーガロイヤルホテル東京
  • Year and Date: 2015-05-22
• [Presentation] PBDE: structure-activity studies for the inhibition of hepatitis C virus NS3 helicase (2014)
  • Author(s): Roy, S.R, K.A. Salam, 古田篤史、野田尚宏、他６名
  • Organizer: アカデミアからの抗がん剤創薬に向けて 天然物の有効利用
  • Place of Presentation: 万国津梁館、沖縄
  • Year and Date: 2014-05-12