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Utilization of microenvironment of nanotubes in in-vitro evolution

Research Project

Project/Area Number 26350978
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemical biology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Kubo Tai  国立研究開発法人産業技術総合研究所, 創薬分子プロファイリング研究センター, 副研究センター長 (10178030)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords試験管内進化 / マイクロ環境 / リフォールディング / 生理活性ペプチド / 分子進化 / 有機ナノチューブ
Outline of Final Research Achievements

We have found that organic nanotubes promoted refolding and thus leading innate activity of peptides when applied to the in-vitro translation reaction of the peptides. The observation was successfully adopted to the in-vitro evolution technology to construct a random peptide library with improved content of active peptides.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 2014

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 7 results) Presentation (19 results) (of which Int'l Joint Research: 7 results,  Invited: 7 results)

  • [Journal Article] Modulated dynamics of PAM-α7 nAChR from X-ray single molecular observations2017

    • Author(s)
      Tomoyuki Baba, Tai Kubo, Sumiko Ohashi, Keigo Ikezaki, Kazuhiro Mio, Hiroshi Sekiguchi, Yuji, C. Sasaki
    • Journal Title

      BIOPHYSICAL JOURNAL

      Volume: 112-3 Pages: 327-327

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Random Peptide Library for Ligand and Drug Discovery2016

    • Author(s)
      Tai Kubo
    • Journal Title

      Toxins and Drug Discovery

      Volume: - Pages: 1-24

    • DOI

      10.1007/978-94-007-6726-3_2-1

    • ISBN
      9789400767263
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A high performance platform based on cDNA display for efficient synthesis of protein fusions and accelerated directed evolution2016

    • Author(s)
      Naimuddin, M., & Kubo, T.
    • Journal Title

      ACS Combinatorial Sci.

      Volume: 18 Issue: 2 Pages: 117-129

    • DOI

      10.1021/acscombsci.5b00139

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (alpha2 beta delta epsilon)2016

    • Author(s)
      Yamauchi, Y., Kimoto, H., Yang, X., Filkin, S., Utkin, Y., Kubo, T., & Inagaki, H.
    • Journal Title

      Biosci. Biotech. and Biochem

      Volume: 80 Issue: 1 Pages: 158-161

    • DOI

      10.1080/09168451.2015.1065169

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Peptidome and transcriptome analysis of the toxin-like peptides in the venom glands of Tarantula Grammostora rosea2015

    • Author(s)
      Kimura, T., & Kubo, T.
    • Journal Title

      Spider Venoms

      Volume: - Pages: 251-270

    • DOI

      10.1007/978-94-007-6646-4_13-1

    • ISBN
      9789400766464
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Directed evolution of three-finger toxin to produce serine protease inhibitors2014

    • Author(s)
      Cai, W., Naimuddin, M., Inagaki, H., Kameyama, K., Ishida, N., & Kubo, T.*
    • Journal Title

      J. Receptors and Signal Transduction

      Volume: 34 Pages: 154-161

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interactions2014

    • Author(s)
      Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, Kubo T.
    • Journal Title

      Anal Chem.

      Volume: 86 Issue: 17 Pages: 8535-8540

    • DOI

      10.1021/ac501601g

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking2014

    • Author(s)
      Sekiguchi, H., Suzuki, Y., Nishino, Y., Kobayashi, S., Shimoyama, Y., Cai, W., Nagata, K., Okada, M., Ichiyanagi, K., Ohta, N., Yagi, N., Miyazawa*, A., Kubo, T.*, & Sasaki, Y.*
    • Journal Title

      Scientific Reports,

      Volume: - Issue: 1

    • DOI

      10.1038/srep06384

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Modulated dynamics of PAM-α7 nAChR from X-ray single molecular observations2017

    • Author(s)
      Tomoyuki Baba, Tai Kubo, Sumiko Ohashi, Keigo Ikezaki, Kazuhiro Mio, Hiroshi Sekiguchi, Yuji C. Sasaki
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      New Orleans Ernest N. Morial Convention Center(米国ルイジアナ州ニューオリンズ)
    • Year and Date
      2017-02-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] X線1分子追跡(DXT)法によるα7ニコチン性アセチルコリン受容体のリアルタイム1分子動態計測2016

    • Author(s)
      馬場知之、大橋澄子、関口博史、池崎圭吾、三尾和弘、佐々木裕次、久保泰
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] リンカー設計およびタンパク質翻訳過程の至適化による試験管内進化技術(cDNAディスプレイ法)の改良と抗VEGF-3本指ペプチドの創製2016

    • Author(s)
      久保泰、Naimuddin Mohammed、多田耕平、大橋澄子、平家勇司、五島直樹
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 2種類の抗サバイビンバインダー(Three-FingerとVHH抗体)のサバイビン阻害活性2016

    • Author(s)
      安斎宏紀、鈴木武尊、四本勇也、木村慎之介、鈴木美穂、久保泰、根本直人
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3D Motion Maps of TRPV1 cation channel depicted by Diffracted X-Ray Tracking Method2016

    • Author(s)
      Kazuhiro Mio, Keigo, Ikezaki, Hiroshi Sekiguchi, Yufuku Matsushita, Tai Kubo, Yuji C. Sasaki
    • Organizer
      第54回生物物理学会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] X-ray imaging of single protein's motion with ultra-high speed and accuracy2016

    • Author(s)
      Tomoyuki Baba, Keigo Ikezaki, Hiroshi Sekiguchi, Tai Kubo, Yuji C. Sasaki
    • Organizer
      The 31st International Congress on High-speed Imaging and Photonics
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府吹田市)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of peptide neurotoxin-based allosteric modulators of nicotinic acetylcholine receptor alpha 7: evaluation by diffracted x-ray tracking method2016

    • Author(s)
      Tai Kubo, Tomoyuki Baba, Sumiko Ohashi, Keigo Ikezaki, Hiroshi Sekiguchi, Yuji C. Sasaki
    • Organizer
      XII Congress of the Pan-America Section of the IST
    • Place of Presentation
      Deauville Hotel(米国フロリダ州マイアミ)
    • Year and Date
      2016-09-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] X線回折点追跡法と試験管内分子進化技術の融合によるチャネル分子の遷移過程識別プローブの創製2016

    • Author(s)
      久保泰
    • Organizer
      第68回埼玉大学脳科学セミナー
    • Place of Presentation
      埼玉大学(埼玉県さいたま市)
    • Year and Date
      2016-07-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イオンチャネルの遷移状態識別プローブ創製に向けて2016

    • Author(s)
      久保泰
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所(大阪府豊中市)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Realtime single molecular motion analysis of nicotinic acetylcholine receptor alpha 7 by diffracted X-ray tracking method2016

    • Author(s)
      Kubo, T., Baba, T., Ikezaki, K., Sekiguchi, H., Nishino, Y., Miyazawa, A., Sasaki, Y.C.
    • Organizer
      60th Annual Meeting of Biophysical Society
    • Place of Presentation
      ロサンゼルス(米国)
    • Year and Date
      2016-02-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Utilization of neurotoxin-inspired peptide libraries in in-vitro evolution, and its proved pluripotency to target GPCRs, proteases and trophic factors2015

    • Author(s)
      Kubo, T., Naimuddin, M., Ono, S., Hirokawa, T.
    • Organizer
      18th World Congress of the International Society on Toxinology
    • Place of Presentation
      オックスフォード(英国)
    • Year and Date
      2015-09-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro evolution from pluripotent peptide libraries with natural neurotoxin scaffolds to target receptors, proteases and trophic factors2015

    • Author(s)
      Kubo, T., Naimuddin, M., Ono, S.
    • Organizer
      29th Annual Symposium of the Protein Society
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Pluripotent random peptide library inspired by natural neurotoxins in the accelerated gene evolution2015

    • Author(s)
      Kubo, T., Naimuddin, M., Ono, S.
    • Organizer
      10th European Biophysics Congress
    • Place of Presentation
      ドレスデン (ドイツ)
    • Year and Date
      2015-07-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] パプアピグミーマルガスネークの生理活性ペプチドおよびタンパク質に関する研究2015

    • Author(s)
      山内瑶子、木本 光、鳥羽通久、久保 泰、稲垣 英利
    • Organizer
      第62回トキシンシンポジウム
    • Place of Presentation
      志摩(三重)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 加速進化型ペプチドのスキャフォールドを利用した分子認識2015

    • Author(s)
      久保泰
    • Organizer
      人工認識分子研究会
    • Place of Presentation
      大阪(大阪)
    • Year and Date
      2015-06-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 加速進化型ペプチドの分子骨格を利用した多能性ペプチドライブラリの利用技術2015

    • Author(s)
      久保泰
    • Organizer
      第20回分子複合医薬研究会
    • Place of Presentation
      池田
    • Year and Date
      2015-02-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 加速進化型ペプチドのscaffoldに基づく多能性ペプチドライブラリの構築と標的特異的分子の創製2014

    • Author(s)
      久保泰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 高速1分子内ダイナミクス計測で捉えるアセチルコリン受容体・機能運動2014

    • Author(s)
      関口博史、徳江真紀、西野有里、一柳光平、久保泰、宮澤淳夫、八木直人、佐々木裕次
    • Organizer
      第14回日本蛋白質科学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Utilization of three-finger peptide library and evolution in vitro toward protease inhibitors.2014

    • Author(s)
      Cai, W., Kubo, T.
    • Organizer
      10th IST Asia Pacific Congress on Animal, Plant and Microbial Toxins
    • Place of Presentation
      Changsha, China
    • Year and Date
      2014-06-16
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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