Functional analysis of the role of hippocampal CA2 region in social behaviour and memory.

• Project/Area Number: 26350979
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Niigata University
• Principal Investigator: Abe Manabu 新潟大学, 脳研究所, 准教授 (10334674)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 海馬 / 記憶 / 遺伝子改変マウス

Outline of Final Research Achievements

The aim of this study was to examine the role of CA2 neurons in memory and social behavior. The function of the hippocampal CA2 region remains largely unknown. To control neural activity by using CA2 neuron-selective gene-targeting system, we made several knock-in lines, in which Cre recombinase or tetracycline transactivator was inserted into the translational initiation site of the targeted genes (Rgs14, Amigo2, Abat and so on) in frame. Additionally, we made a new line expressing tdTomato reporter under control of Cre recombinase. The mutant lines developed in this study could be useful tools for analysis of higher brain functions.

Research Products

• [Journal Article] Distribution of corticotropin-releasing factor neurons in the mouse brain : a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse. (2018)
  • Author(s): Kono J, Konno K, Talukder AH, Fuse T, Abe M, Uchida K, Horio S, Sakimura K, Watanabe M, Itoi K.
  • Journal Title: Brain Struct Funct. Volume: 222 Issue: 1 Pages: 1705-1732
  • DOI: 10.1007/s00429-016-1303-0
  • Peer Reviewed / Open Access
• [Journal Article] YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation. (2017)
  • Author(s): Shimizu T, Osanai Y, Tanaka KF, Abe M, Natsume R, Sakimura K, Ikenaka K.
  • Journal Title: Glia. Volume: 65 Issue: 2 Pages: 360-374
  • DOI: 10.1002/glia.23096
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Forward genetic analysis of sleep in randomly mutagenized mice. (2017)
  • Author(s): Funato H, et al.
  • Journal Title: Nature. Volume: 539 Issue: 46 Pages: 378-383
  • DOI: 10.1038/nature20142
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impaired auditory processing and altered structure of the endbulb of Held synapse in mice lacking the GluA3 subunit of AMPA receptors. (2017)
  • Author(s): Garcia-Hernandez S, Abe M, Sakimura K, Rubio ME.
  • Journal Title: Hear Res. Volume: 344 Pages: 284-294
  • DOI: 10.1016/j.heares.2016.12.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts (2017)
  • Author(s): Danno S, Kubouchi K, Mehruba M, Abe M, Natsume R, Sakimura K, Eguchi S, Oka M, Hirashima M, Yasuda H, Mukai H.
  • Journal Title: Genes to Cell Volume: 22 Pages: 220-236
  • DOI: 10.1111/gtc.12470
  • Peer Reviewed
• [Journal Article] The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model (2017)
  • Author(s): Shimizu T, Wisessmith W, Li J, Abe M, Sakimura K, Chetsawang B, Sahara Y, Tohyama K, Tanaka KF, Ikenaka K.
  • Journal Title: GLIA Volume: 65(6) Pages: 917-930
  • DOI: 10.1002/glia.23134
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TARP γ-2 and γ-8 differentially control AMPAR density across Schaffer collateral/commissural synapsee in the hippocampal CA1 area. (2016)
  • Author(s): Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M.
  • Journal Title: J Neurosci. Volume: 36 Issue: 15 Pages: 4296-312
  • DOI: 10.1523/jneurosci.4178-15.2016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transsynaptic modulation of kainate receptor functions by Clq-like proteins. (2016)
  • Author(s): Matsuda K, Budisantoso T, Mitakidis N, Sugaya Y, Miura E, Kakegawa W, Yamasaki M, Konno K, Uchigashima M, Abe M, Watanabe I, Kano M, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M
  • Journal Title: Neuron Volume: 90 Pages: 752-67
  • DOI: 10.1016/j.neuron.2016.04.001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Tt119-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating. (2016)
  • Author(s): Konno A, Ikegami K, Konishi Y, Yang HJ, Abe M, Yamazaki M, Sakimura K, Yao I, Shiba K, Inaba K, Setou M
  • Journal Title: J Cell Sci. Volume: 129 Issue: 14 Pages: 2757-2766
  • DOI: 10.1242/jcs.185983
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] SAD-B phosphorylation of CAST controls active zone vesicle recycling for synaptic depression. (2016)
  • Author(s): Mochida S, Hida Y, Tanifuji S, Hagiwara A, Hamada S, Abe M, Ma H, Yasumura M, Kitajima I, Sakimura K, Ohtsuka T
  • Journal Title: Cell Rep. Volume: 16 Issue: 11 Pages: 2901-13
  • DOI: 10.1016/j.celrep.2016.08.020
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury. (2016)
  • Author(s): Katano T, Fukuda M, Furue H, Yamazaki M, Abe M, Watanabe M, Nishida K, Yao I, Yamada A, Hata Y, Okumura N, Nakazawa T, Yamamoto T, Sakimura K, Takao T, Ito S.
  • Journal Title: eNeuro. Volume: 3 Issue: 5 Pages: 0110-16
  • DOI: 10.1523/eneuro.0110-16.2016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Roles of Cbln1 in Non-Motor Functions of Mice. (2016)
  • Author(s): Otsuka S, Konno K, Abe M, Motohashi J, Kohda K, Sakimura K, Watanabe M, Yuzaki M.
  • Journal Title: J Neurosci. Volume: 36 Pages: 11801-11816
  • Peer Reviewed
• [Journal Article] A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance (2016)
  • Author(s): Parajuli LK, Ageta-Ishihara N, Ageta H, Fukazawa Y, Kinoshita M.
  • Journal Title: Nature Commun Volume: 136 Pages: 10090-10090
  • DOI: 10.1038/ncomms10090
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Determination of kainate receptor subunit ratios in mouse brain using novel chimeric protein standards. (2016)
  • Author(s): Watanabe-Iida I, Konno K, Akashi K, Abe M, Natsume R, Watanabe M, Sakimura K.
  • Journal Title: J Neurochem. Volume: 136 Pages: 295-305
  • DOI: 10.1111/jnc.13384
  • Peer Reviewed / Open Access
• [Journal Article] Heterozygous disruption of Autism susceptibility candidate 2 causes impaired emotional control and cognitive memory. (2015)
  • Author(s): Hori K, Nagai T, Shan W, Sakamoto A, Abe M, Yamazaki M, Sakimura K, Yamada K, Hoshino M.
  • Journal Title: PLOS ONE Volume: 30
  • DOI: 10.1371/journal.pone.0145979
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] STAT5 orchestrates local epigenetic changes for chromatin accessibility and rearrangements by direct binding to the TCRγ locus (2015)
  • Author(s): Wagatsuma K, Tani-Ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K.
  • Journal Title: J Immunol Volume: 195 Pages: 1804-14
  • DOI: 10.4049/jimmunol.1302456
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Horizontal Basal Cell-Specific Deletion of Pax6 Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury. (2015)
  • Author(s): Suzuki J, Sakurai K, Yamazaki M, Abe M, Inada H, Sakimura K, Katori Y, Osumi N.
  • Journal Title: Stem Cells Dev Volume: 24 Pages: 1923-1933
  • DOI: 10.1089/scd.2015.0011
  • Peer Reviewed / Open Access
• [Journal Article] Cdk5/p35 is required for motor coordination and cerebellar plasticity. (2014)
  • Author(s): He X, Ishizeki M, Mita N, Wada S, Araki Y, Ogura H, Abe M, Yamazaki M, Sakimura K, Mikoshiba K, Inoue T, Ohshima T.
  • Journal Title: J Neurochem Volume: 131(1) Pages: 53-64
  • DOI: 10.1111/jnc.12756
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Visualization of Corticotropin-Releasing Factor Neurons by Fluorescent Proteins in the Mouse Brain and Characterization of Labeled Neurons in the Paraventricular Nucleus of the Hypothalamus (2014)
  • Author(s): Itoi K, Talukder AH, Fuse T, Kaneko T, Ozawa R, Sato T, Sugaya T, Uchida K, Yamazaki M, Abe M, Natsume R, Sakimura K.
  • Journal Title: Endocrinology Volume: 155(10) Pages: 4054-4060
  • DOI: 10.1210/en.2014-1182
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expansion of stochastic expression repertoire by tandem duplication in mouse Protocadherin-a cluster. (2014)
  • Author(s): Kaneko R, Abe M, Hirabayashi T, Uchimura A, Sakimura K, Yanagawa Y, Yagi T.
  • Journal Title: Sci Rep Volume: 4 Pages: 6263-6263
  • DOI: 10.1038/srep06263
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expansion of stochastic expression repertoire by tandem duplication in mouse Protocadherin-α cluster. (2014)
  • Author(s): Misato Yasumura, Tomoyuki Yoshida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kouta Kanno, Takeshi Uemura, Keizo Takao, Kenji Sakimura, Takefumi Kikusui, Tsuyoshi Miyakawa, Masayoshi Mishina
  • Journal Title: Sci Rep. Volume: 4: 6613 Pages: 6613-6613
  • DOI: 10.1038/srep06613
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Proteasome dysfunction induces muscle growth defects and protein aggregation. (2014)
  • Author(s): Kitajima Y, Tashiro Y, Suzuki N, Warita H, Kato M, Tateyama M, Ando R, Izumi R, Yamazaki M, Abe M, Sakimura K, Ito H, Urushitani M, Nagatomi R, Takahashi R, and Aoki M
  • Journal Title: J Cell Sci Volume: 127 Pages: 5204-17
  • DOI: 10.1242/jcs.150961
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] CRISPR/Cas9-mediated endogenous protein tagging in mice by zygote electroporation. (2016)
  • Author(s): Abe M, Natsume R, Nakatsukasa E, Sakimura K.
  • Organizer: 日本ゲノム編集学会第1回大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2016-09-06
• [Presentation] AMPA受容体パルミトイル化制御による脳機能バランス維持 (2015)
  • Author(s): 山下真梨子、奥野浩行、阿部学、山崎真弥、夏目里恵、崎村建司、星野幹雄、三品昌美、林崇
  • Organizer: BMB2015
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-03
• [Presentation] Molecular determinants underlying heterogeneity in AMPAR content across CA1 Schaffer collateral synapses. (2015)
  • Author(s): M.Yamasaki, M.Fukaya, M.Yamazaki, M.Abe, K.Sakimura, M.Watanabe
  • Organizer: Neuroscience2015
  • Place of Presentation: Chicago,USA
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Visualizing single-neuron identity defined by Pcdh-b cluster in mouse brain (2015)
  • Author(s): 金子涼輔、阿部学、渡辺雅彦、崎村建司、柳川右千夫、八木健
  • Organizer: 第58回日本神経化学会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-09-13
• [Presentation] Progranulin regulates climbing fiber synapse elimination during postnatal cerebellar development (2015)
  • Author(s): 上阪直史、阿部学、山崎真弥、今野幸太郎、三國貴康、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: 第38回日本神経科学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-07-28
• [Presentation] グルタミン酸受容体サブユニット定量のための標準タンパク質の調整 (2015)
  • Author(s): 西川千晶、渡辺和泉、中本千尋、鈴木康浩、阿部学、崎村建司
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-03-19
• [Presentation] Gene targeting and genome editing in C57BL/6 mouse strain for brain research (2015)
  • Author(s): Manabu Abe
  • Organizer: BRI International Symposium 2015
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2015-03-05
  • Invited
• [Presentation] Functional lodd of Nna1 is resposible for Purkinje degeneration. (2014)
  • Author(s): Li Zhou, Manabu Abe,Maya Yamazaki,Rie Natsume,Kenji Sakimura
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-27
• [Presentation] The dynamics and function of two glutamate receptor delta subunits. (2014)
  • Author(s): C.Nakamoto, I.Watanabe,R.Natsume,E.Nakatsukasa,K.Konno,M.Abe,M.Watanabe,K.Sakimura
  • Organizer: SFN2014
  • Place of Presentation: WashingtonDC,USA
  • Year and Date: 2014-11-16
• [Presentation] 発達期および成体マウスにおける小脳顆粒細胞選択的NMDA状態欠損は運動障害を引き起こす (2014)
  • Author(s): Peng Fei、阿部学、薄井宏、夏目里恵、崎村建司
  • Organizer: 第57回日本神経化学会大会
  • Place of Presentation: 奈良県奈良市
  • Year and Date: 2014-09-30
• [Remarks] 新潟大学脳研究所 研究分野紹介 細胞神経生物学分野
  • URL: http://www.bri.niigata-u.ac.jp/field/cellular/index.html
• [Remarks] 新潟大学脳研究所ホームページ＞研究分野紹介＞細胞神経生物学分野
  • URL: http://www.bri.niigata-u.ac.jp/field/cellular/index.html
• [Remarks] 新潟大学脳研究所 細胞神経生物学分野
  • URL: http://www.bri.niigata-u.ac.jp/cellular/