| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In order to enhance the ability of single-molecule fluorescence imaging (SMFI) that enables to detect binding and dissociation events of bio-molecules in real time using zero-mode waveguides (ZMWs), we propose a novel technique to combine fluorescence resonance energy transfer (FRET) and SMFI with ZMWs, and proved experimentally that the proposed method is feasible. We also tried to extend the ability of this technique by demonstrating SMFI of individual DNA aptamers for sensing application, SMFI of DNA strand scission by endonuclease, and in-situ cell patterning.
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