Melt-like growth of protein crystals
| Project/Area Number |
26390054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Crystal engineering
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤原 貴久 徳島大学, 大学院理工学研究部, 教務補佐員 (10648695)
佐藤 正英 金沢大学, 総合メディア基盤センター, 教授 (20306533)
佐崎 元 北海道大学, 低温科学研究所, 教授 (60261509)
勝野 弘康 立命館大学, 理工学部, 助教 (70377927)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脱塩 / 遠心濃縮 / 結晶構造解析 / 液液相分離 / 融液様成長 / リゾチーム / グリセロール / グルコースイソメラーゼ / 酵素活性 / 分子ステップ |
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| Outline of Final Research Achievements |
Salt-free protein crystallization method with centrifugal concentration apparatus has been developed. With this method, nucleation of hen egg-white lysozyme (HEWL) crystals occurs in hyper-concentrated liquid phase obtained by liquid-liquid phase separation during centrifugal concentration processes. We could successfully observed molecular steps on the obtained crystal surface. Three-dimensional structure of lysozyme molecule obtained in this method is significantly different from that obtained by normal salting-out method.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Correction of the equilibrium temperature caused by slight evaporation of water in protein crystal growth cells during long-term space experiments at International Space Station2015
Author(s)
Takahisa Fujiwara, Yoshihisa Suzuki, Izumi Yoshizaki, Katsuo Tsukamoto, Kenta Murayama, Seijiro Fukuyama, Kouhei Hosokawa, Kentaro Oshi, Daisuke Ito, Tomoya Yamazaki, Masaru Tachibana and Hitoshi Miura
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Journal Title
Review of Scientific Instruments
Volume: 86
Issue: 8
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Recent Advances on the Ground-Based Experiments of Protein Crystallization after the NanoStep Project2015
Author(s)
Yoshihisa Suzuki, Katsuo Tsukamoto, Takahisa Fujiwara, Tomohiro Shiomoto, Daido Nakahashi, Izumi Yoshizaki, Seijiro Fukuyama, Masaru Tachibana, Haruhiko Koizumi, Shin-ichiro Yanagiya, Yasutomo Arai and Makoto Natuisaka
Organizer
The Joint Conference of 6th International Symposium on Phshycs in Space & 10th Inerntional Conference on Two-Phase System for Space and Ground Applications
Place of Presentation
同志社大学（京都府京都市）
Year and Date
2015-09-18
Related Report
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