Development of techniques for patterning of differentiating cells derived from neural stem cells
| Project/Area Number |
26420799
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Mori Hideki 大阪府立大学, 理学(系)研究科(研究院), 准教授 (30450894)
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| Co-Investigator(Kenkyū-buntansha) |
原 正之 大阪府立大学, 理学(系)研究科(研究院), 教授 (50344172)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | neural stem cell / viscoelasiticity / collagen gel / endothelial cell / UV-irradiation / cell patterning / コラーゲン / 神経幹細胞 / 血管内皮細胞 / 紫外線照射 / ハイドロゲル / 粘弾性 / パターニング / パターンニング |
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| Outline of Final Research Achievements |
Collagen gels were irradiated by ultra violet (UV) light for intermolecularly-cross-linking. Young’s modulus of collagen gels increased after UV-irradiation. Collagen gels were irradiated with stripe pattern using photo-masking materials. Brain capillary endothelial cells (bEnd.3) were cultured on the UV-irradiated collagen gels. bEnd.3 cells were adhered on UV-irradiated collagen gel with stripe pattern. Next, mouse neural stem/progenitor cells formed neurosphere were seeded on UV-irradiated collagen gels with stripe patterned bEnd.3 cells. After the incubation with the differentiation medium for one week, stripe pattern consisted of bEnd.3 cells and differentiating cells form neural stem/progenitor cells were formed on UV-irradiated collagen gels.
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Report
(4 results)
Research Products
(15 results)
