| Project/Area Number |
26430123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NOMIZU Motoyoshi 東京薬科大学, 薬学部, 教授 (00311522)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ラミニン / 基底膜 / 細胞外マトリックス / 癌 / 細胞接着 / 細胞運動 / ルテラン / インテグリン |
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| Outline of Final Research Achievements |
Epithelial cells stably anchor to basement membrane, whereas malignant tumors pass through it to achieve metastasis. Of basement membrane components, laminin-511 (alpha5, beta1, gamma1; LM-511) is a major isoform in many adult tissues. Several studies have shown that LM-511 promotes not only cell adhesion but also tumor cell migration. Thus, LM-511 can be viewed like two distinct molecules in normal vs. tumor cells. In this study, we focused on 1) the assembly of basement membrane containing LM-511 in tumor microenviroments and 2) the migration of tumor cells adhering to LM-511. The amino acid sequences active for endothelial cell tube formation were identified using synthetic peptides-derived from laminin alpha5 N-terminus. Our results also showed that endogenous LM-511 promotes the formation of cyst structure accompanying with basement membrane. Moreover, we found that the inside-out signal is involved in the transition from static to migratory cell behaviors on LM-511.
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