| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In order to use the TRUE gene silence method using sgRNA for treatment of hematologic malignancies, sgRNA was prepared using various tumor - associated mRNA as a target, and suppression of cell proliferation and apoptosis induction were examined. In several leukemia cell lines, the effect of sgRNA on WT1 and Bcl-2 was confirmed. It was also confirmed that this effect varied depending on the concentration of glucose in the culture medium.We could establish condition setting to predict the effect of sgRNA.
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