| Project/Area Number |
26430179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor therapeutics
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
Kuzushima Kiyotaka 愛知県がんセンター(研究所), 腫瘍免疫学部, 部長 (30311442)
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| Co-Investigator(Kenkyū-buntansha) |
近藤 英作 新潟大学, 分子細胞病理学分野, 教授 (30252951)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 樹状細胞 / 細胞透過性ペプチド / 細胞傷害性Tリンパ球 / 免疫療法 / がんワクチン / 抗原提示 |
|---|
| Outline of Final Research Achievements |
By means of a peptide-conjugated cDNA library screening method, several dendritic cell (DC)-selected, cell penetrating peptides (CPP) have been identified. These CPP, namely H03L and F04L, when fused to a CTL target antigen, exert presentation more effectively on DC than on fibroblast cells.The antigen presentation by H03L and F04L on DC was superior to that of R10, which is a non-selective CPP. Then H03L and F04L were respectively fused to FITC or recombinant green fluorescence protein(GFP). R10 was used as a (non-selective) CPP control. These fluorescent molecules were pulsed on in vitro induced DC, peripheral lymphocyte, EBV-infected B-lymphoblastoid cells or fibroblast cells. After incubated and washed, they were analyzed on a flow cytometer. Both H03L and F04L effectively penetrated not only into DC but also peripheral monocytes and the B-lymphoblastoid cells. These results indicate the identified CPP are not exclusively selective to DC, but to myeloid lineage cells.
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