| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
1) Raccoon; Five synthetic peptides were produced with reference to raccoon ZP3. Immune-histochemistry was used to assess antibody binding of sera from rabbits immunized against each of the five peptides to ovaries of raccoon and the other 9 species. Rabbit sera against three out of the five peptides showed a raccoon-specific reaction. These three peptides would be potential antigens for immunocontraceptive vaccines.
2) Mongoose; Two synthetic peptides (A and B) with reference to mongoose ZPC were injected into female mongooses, and blood samples were collected to verify immunogenicity. Mongooses injected with peptide A showed a marked increase in antibody titer, which lasted for 8 months; in contrast, peptide B caused relatively mild increase in antibody titer, lasting for 5 months. Immune-histochemical analysis showed that the antibodies against each peptide could recognize ovary-derived proteins. Peptide A and B would be potential antigens for immunocontraceptive vaccines.
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