Development of immunocontraceptive vaccines based on zona pellucida antigens for population control of feral raccoons and small Indian mongooses in Japan
| Project/Area Number |
26430203
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservation of biological resources
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| Research Institution | Gifu University |
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Principal Investigator |
ASANO MAKOTO 岐阜大学, 応用生物科学部, 准教授 (30377692)
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| Research Collaborator |
KUNINAGA Natoshi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 外来種 / 避妊ワクチン / アライグマ / マングース / 特定外来生物 / 免疫学的避妊 / ワクチン / 避妊 |
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| Outline of Final Research Achievements |
1) Raccoon; Five synthetic peptides were produced with reference to raccoon ZP3. Immune-histochemistry was used to assess antibody binding of sera from rabbits immunized against each of the five peptides to ovaries of raccoon and the other 9 species. Rabbit sera against three out of the five peptides showed a raccoon-specific reaction. These three peptides would be potential antigens for immunocontraceptive vaccines.
2) Mongoose; Two synthetic peptides (A and B) with reference to mongoose ZPC were injected into female mongooses, and blood samples were collected to verify immunogenicity. Mongooses injected with peptide A showed a marked increase in antibody titer, which lasted for 8 months; in contrast, peptide B caused relatively mild increase in antibody titer, lasting for 5 months. Immune-histochemical analysis showed that the antibodies against each peptide could recognize ovary-derived proteins. Peptide A and B would be potential antigens for immunocontraceptive vaccines.
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Report
(4 results)
Research Products
(11 results)
