Studies on the vacuum-drying of mouse preimplantation embryos, ES cells and iPS cells
Project/Area Number |
26430207
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservation of biological resources
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Research Institution | Juntendo University |
Principal Investigator |
Tada Norihiro 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (50338315)
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Project Period (FY) |
2014-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | マウス / 初期胚 / ES細胞 / iPS細胞 / 真空乾燥 / 室温保存 / トレハロース / CRISPR/Cas9 / 受精卵 / エネルギーフリー / 生殖機能細胞 / 卵子 / 精子 / 長期 |
Outline of Final Research Achievements |
In this study, we examined for the purpose of development of novel technique on vacuum drying-room temperature preservation in mouse preimplantation stage embryos. Mouse embryos were vacuum-dried with stock solution containing various concentration of trehalose and preserved at room temperature for 48 h. After rehydration, the efficiency of survived embryos were very low when none of embryos vacuum-dried and preserved at room temperature for 72 h were survived. In addition, knockin (KI) mice inserted trehalose transporter gene into safe harbor region of ROSA26 gene locus were generated by CRISPR/Cas9 system. Preimplantation stage embryos from trehalose transporter-KI mice will be vacuum dried and preserved at room temperature for confirming their survivability after rehydration.
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Academic Significance and Societal Importance of the Research Achievements |
卵子、受精卵、初期胚、ES細胞及びiPS細胞のような生殖機能細胞は、いずれも、真空乾燥・室温保存の成功例は、まったく認められないので、極めて独創的であり、各細胞腫の核のみならず、細胞質及び細胞膜等の乾燥に対する耐性機構が明らかになるので、学術的な意義は大きい。また、現在、生殖機能細胞の保存は、すべて凍結保存であるが、本研究で、真空乾燥・室温保存の有効性を明らかにできれば、この技術を各種のバンクへ適用すると共に、野生動物の絶滅危惧種の生殖機能細胞を室温保存することによって、遺伝資源の開発・保全に寄与できる可能性がある。
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Report
(6 results)
Research Products
(26 results)
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[Journal Article] Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis2019
Author(s)
Asada Y, Okano M, Ishida W, Iwamoto S, Fukuda K, Hirakata T, Tada N, Fukushima A, Ebihara N, Kudo A, Matsuda A.
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Journal Title
Allergology International
Volume: 68
Issue: 2
Pages: 233-239
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hippocampal accumulation of leucine-rich alpha2-glycoprotein contributes to age-related memory impairment.2017
Author(s)
Chihiro Akiba, Madoka Nakajima, Masakazu Miyajima, Ikuko Ogino, Eri Nakamura, Fumio Kanai, Norihiro Tada, Miyuki Kunichika, Mitsutaka Yoshida, Kinya Nishimura, Ritsuko Inoue, Masami Miura, Akihide Kondo, Hidenori Sugano, Hajime Arai
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Journal Title
Neurobiology of Aging
Volume: 60
Pages: 11-19
Related Report
Peer Reviewed
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[Journal Article] Cathepsin D in podocytes plays an important role in the pathogenesis of proteinuria and chronic kidney disease2016
Author(s)
Nonaka-Yamamoto K, Koike M, Asanuma K, Akiba-Takagi M,1 Trejo JAO, Seki T, Hidaka T, ◎Ichimura K, Sakai T, Tada N, Ueno T, Uchiyama Y, Tomino Y.
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Journal Title
J Am Soc Nephrol
Volume: in press
Issue: 9
Pages: 2685-2700
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Omega 3 Polyunsaturated Fatty Acids Suppress the Development of Aortic Aneurysms Through the Inhibition of Macrophage-Mediated Inflammation.2015
Author(s)
Takuma Yoshihara, Kazunori Shimada, Kosuke Fukao, Eiryu Sai, Yayoi Sato-Okabayashi, Rie Matsumori, Tomoyuki Shiozawa, Hamad Alshahi, Tetsuro Miyazaki, Norihiro Tada, Hiroyuki Daida
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Journal Title
Circ J
Volume: 79
Pages: 1470-1478
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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