| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The nucleosome is the fundamental structural units of eukaryotic chromatin. Immediately before cell division, duplicated nucleosome fibers are organized into a pair of rod-shaped structures within a mitotic chromosome. However, it remains unclear whether nucleosome assembly is indeed an essential prerequisite for mitotic chromosome assembly. To settle this naive question, I have established chromosome reconstitution system with a minimum set of purified proteins and the cell-free chromosome assembly assay made from frog eggs. A substantial set of our results convincingly indicates that, although formation of chromosome axes does not rely on nucleosomes, functional interaction between nucleosomes and the multi-protein complexes termed condensins is required for assembly of fully compact chromosomes.
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