| Project/Area Number |
26440015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University (2016)
National Institute of Information and Communications Technology (2014-2015) |
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Principal Investigator |
OGAWA Hidesato 大阪大学, 生命機能研究科, 特任准教授(常勤) (20370132)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEUCHI Jun 東京大学, 分子細胞生物学研究所, 准教授 (10451999)
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| Research Collaborator |
TSUCHIYA Megumi 大阪大学, 生命機能研究科, 特任助教 (00390691)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | クロマチン構造変換 / 核内受容体 / 転写因子 / オートファジー / クロマチン / 転写制御 / 心筋分化 / 細胞周期 / 翻訳後修飾 / 核内構造体 / 転写複合体 |
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| Outline of Final Research Achievements |
Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their transcriptional activity. In this study, we identified p62 as a major interacting protein partner for ARIP4 in the nucleus. Nuclear magnetic resonance analysis demonstrated that ARIP4 interacts directly with the ubiquitin-associated (UBA) domain of p62. ARIP4 and ubiquitin both bind to similar amino acid residues within UBA domains; therefore, these proteins may possess a similar surface structure at their UBA-binding interfaces. We also found that p62 is required for the regulation of ARIP4 protein levels under nutrient starvation conditions. We propose that p62 is a novel binding partner for ARIP4, and that its binding regulates the cellular protein level of ARIP4 under conditions of metabolic stress.
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