| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
Exosome is a subtype of extracellular vesicle, released from various cells including brain neurons. Neuronal cell line (N2a)-derived exosomes can bind amyloid-beta peptide (Abeta) in GSL-dependent manner and clear it through transporting into microglia. In this study, we validated that mouse neuronal exosomes, not glial ones, had abundant GSLs and could capture Abeta. Infusion of the neuronal exosomes into APP transgenic mice decreased Abeta and amyloid depositions, similar to previously reported neuroblastoma-derived exosomes. However, synthetic liposomes containing neuronal GSLs had no potency to bind Abeta. Future challenge is to construct exosome-like liposomes referred to total lipidomics data of neuronal exosomes.
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