Post-translational regulation of Hes1 in nerual stem cells
Project/Area Number |
26440122
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Kyoto University |
Principal Investigator |
Kobayashi Taeko 京都大学, ウイルス・再生医科学研究所, 助教 (40402804)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | Hes1 / オシレーション / 翻訳後修飾 / 幹細胞分化 / 発現振動 / リプレッサー活性 / タンパク質分解 / 神経幹細胞 |
Outline of Final Research Achievements |
Hes1 regulates the maintenance and the differentiation of stem cells by its dynamic expression. Previous studies demonstrated that Hes1 expression oscillates in stem cells, and that the oscillation contributes to their potency and differentiation fates. However, the molecular mechanisms regulating the oscillatory expression of Hes1 protein have not been fully identified. We have focused on the post-translational regulation of Hes1 protein and tried to identify regulatory factors from the binding partners of Hes1 protein. We identified Usp27x and its homologs as new deubiquitinases (DUBs) of Hes1. We reported that these DUBs stabilize and modulate Hes1 protein dynamics by removing ubiquitin molecules, and thereby regulate neuronal differentiation of stem cells (Kobayashi et al, 2015). Moreover, we identified some amino acid residues crucial for Hes1 functions by post-translational regulation. We also identified new factors bound to recombinant Hes1.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Deubiquitinating enzymes regulate Hes1 stability and neuronal differentiation.2015
Author(s)
Kobayashi, T., Iwamoto, Y., Takashima, K., Isomura, A., Kosodo, Y., Kawakami, K., Nishioka, T., Kaibuchi, K., and Kageyama, R.
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Journal Title
FEBS J.
Volume: 282
Pages: 2475-2487
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Novel and robust transplantation reveals the acquisition of polarized processes by cortical cells derived from mouse and human pluripotent stem cells.2014
Author(s)
Nagashima, F., Suzuki, I. K., Shitamukai, A., Sakaguchi, H., Iwashita, M., Kobayashi, T., Tone, S., Toida, K., Vanderhaeghen, P. and Kosodo, Y.
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Journal Title
Stem Cells Dev.
Volume: 23
Pages: 2129-2142
DOI
Related Report
Peer Reviewed
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