Identification of signaling pathways regulating multiciliate cell differentiation, maturation and function in early embryos

• Project/Area Number: 26440123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: KUSAKABE Morioh 京都大学, 生命科学研究科, 講師 (80378843)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アフリカツメガエル / シグナル伝達 / 多繊毛

Outline of Final Research Achievements

Multiciliate cells, which are found in epithelia of various organs in vertebrates, generate extracellular fluid flow along epithelial surfaces by several hundreds of motile cilia. In this study, we show that mab21-l3, which has been identified as a Ras-repressed gene in our previous study, is required for early specification of multiciliate cells and ion-transporting ionocytes. Moreover, we find that an atypical MAPK member, ERK7, regulates ciliogenesis by phosphorylating the actin regulator CapZIP.

Research Products

• [Journal Article] ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled (2015)
  • Author(s): Miyatake K. et al.
  • Journal Title: Nature Commun. Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms7666
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] mab21-l3 regulates cell fate specification of multiciliate cells and ionocytes (2015)
  • Author(s): Chika Takahashi, Morioh Kusakabe, Toshiyasu Suzuki, Koichi Miyatake and Eisuke Nishida
  • Journal Title: Nature Communications Volume: 6 (6017) Issue: 1 Pages: 1-13
  • DOI: 10.1038/ncomms7017
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] A Notch-repressed gene mab21-l3 is a key regulator for cell fate specification of multiciliate cells and ionocytes. (2015)
  • Author(s): Morioh Kusakabe, Chika Takahashi, Eisuke Nishida
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド、神戸、日本
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] ERK7, a novel regulator of ciliogenesis, is required for basal body migration. (2014)
  • Author(s): Miyatake K, Kusakabe M, Takahashi C, Nishida E.
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: Pacific Grove, California, USA
  • Year and Date: 2014-08-24 – 2014-08-28
• [Presentation] Xenopus mab21-l3 is required for cell fate specification of multiciliate cells and ionocytes. (2014)
  • Author(s): Takahashi C, Kusakabe M, Suzuki T, Miyatake K, Nishida E.
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: Pacific Grove, California, USA
  • Year and Date: 2014-08-24 – 2014-08-28