Recognition system in pollen-pistil interaction in Brassicaseae

• Project/Area Number: 26440145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka University
• Principal Investigator: Iwano Megumi 大阪大学, 産業科学研究所, 特任助教(常勤) (50160130)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アブラナ科植物 / 和合受粉 / 液胞 / 小胞体 / トランスゴルジネットワーク / カルシウム輸送体 / シロイヌナズナ / ゼニゴケ / 生殖 / 形態形成 / イメージング / 受粉過程 / カルシウム / 花粉管ガイダンス / 発光イメージング / カルシウムシグナリング / カルシウムポンプ / カルシウムチャネル / ライブセルイメージング / ゴルジ体 / ベシクル輸送

Outline of Final Research Achievements

In the Brassicaceae, intraspecific non-self pollen (compatible pollen) can germinate and grow into the stigmatic papilla cells, while self pollen or interspecific pollen are rejected at this stage. However, the mechanisms underlying this selective acceptance of compatible pollen remain unknown. Here, analysis of live-cell imaging and three dimensional tomography using Scanning Transmission Electron Microscope (STEM) visualized the interaction between cell membrane and interacellular memberane during compatible pollination, and biochemical analysis clarified the molecules interacting with Ca2+ transporter in the papilla cell membrane.

Research Products

• [Journal Article] Bioluminescent Low-Affinity Ca2+ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells (2018)
  • Author(s): Hossain Md Nadim、Suzuki Kazushi、Iwano Megumi、Matsuda Tomoki、Nagai Takeharu
  • Journal Title: ACS Chemical Biology Volume: 13 Issue: 7 Pages: 1862-1871
  • DOI: 10.1021/acschembio.7b01014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4 (2017)
  • Author(s): Yoshida N, Yano T, Kedo K, Fujiyoshi T, Nagai R, Iwano M, Taguchi E, Nishida T, Takagi H.
  • Journal Title: Appl. Microbiol. Biotech. Volume: 101 Issue: 1 Pages: 331-340
  • DOI: 10.1007/s00253-016-7883-z
  • Peer Reviewed
• [Journal Article] Cullin1-P is an essential component of non-self recognition system in self-sncompatibility in Petunia (2016)
  • Author(s): Kubo K, Tsukahara M, Fujii S, Murase K, Wada Y, Entani T, Iwano M, Takayama S
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 11 Pages: 2403-2416
  • DOI: 10.1093/pcp/pcw152
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Calcium signalling mediates self-incompatibility response in the Brassicaceae (2015)
  • Author(s): Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Igarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takehisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, Isogai A, Takayama Seiji
  • Journal Title: Nature Plants Volume: 1 Issue: 1 Pages: 15128-15136
  • DOI: 10.1038/nplants.2015.143
  • NAID: 120005716858
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nuclear membrane localization during pollen development and apex-focused polarity establishment of SYP124/125 during pollen germination in Arabidopsis thaliana (2015)
  • Author(s): Ichikawa M, Iwano M, Sato MH.
  • Journal Title: Plant Reprod. Volume: 28 Issue: 3-4 Pages: 143-151
  • DOI: 10.1007/s00497-015-0265-3
  • Peer Reviewed
• [Journal Article] Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis (2015)
  • Author(s): 1)Kaya H, Iwano M, Takeda S, Kanaoka MM, Kimura S, Higashiyama T, Abe M, Kuchitsu K.
  • Journal Title: Plant Signaling Behavior Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.4161/15592324.2014.989050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination. (2015)
  • Author(s): Matsuda, T., Matsushima, M., Nabemoto, M., Osaka, M., Sakazono, S., Masuko-Suzuki, H., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K. K., Okumura, K., Suzuki, G., Watanabe, M., Suwabe, K.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 4 Pages: 663-673
  • DOI: 10.1093/pcp/pcu209
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Self/Non-self recognition mechanisms in sexual reproduction : New insight into the self-incompatibility system shared by flowering plants and hermaphroditic animals (2014)
  • Author(s): Sawada H., Morita, M., and Iwano, M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: (in press) Issue: 3 Pages: 1142-1148
  • DOI: 10.1016/j.bbrc.2014.05.099
  • Peer Reviewed / Open Access
• [Presentation] ライブイメージングのための発光植物の開発 (2017)
  • Author(s): 岩野恵
  • Organizer: 日本細胞生物学会大会
  • Invited
• [Presentation] An Investigation of Effect of Bioluminescent Light on Human using Electroencephalogram (2016)
  • Author(s): Thammasan N, Iwano M, Moriyama K, Fukui K, Kawintiranon K, Buatong Y, Inagaki S, Nagai T, Numao M.
  • Organizer: The 23nd International Display Workshop in conjunction with Asia Display (IDW/AD2016)
  • Place of Presentation: Fukuoka
  • Year and Date: 2016-12-07
  • Int'l Joint Research
• [Presentation] Development of brilliant light-emitting Marchantia polymorpha for single-cell and whole-plant imaging (2016)
  • Author(s): Iwano M, Nishihama R, Suzuki K, Ishida S, Kaku T, Kochi T, Nagai T.
  • Organizer: ISBC2016 (19th International Symposium on Bioluminescence & Chemiluminescence)
  • Place of Presentation: Tsukuba International Congress Center
  • Year and Date: 2016-05-29
  • Int'l Joint Research
• [Presentation] 高光度発光性植物による新規イメージングシステムの開発 (2016)
  • Author(s): 岩野恵, 西浜竜一, 鈴木和志, 末次憲之, 石田咲子, 加来友美, 河内孝之, 永井健治
  • Organizer: 第57回日本植物生理学会大会
  • Place of Presentation: 岩手大学、上田キャンパス
  • Year and Date: 2016-03-18
• [Presentation] Ca2+ monitoring upon wounding stress in plants by luminescence probes (2015)
  • Author(s): Iwano M, Suetsugu N, Nishihama R, Kaku T, Kochi T, Nagai T
  • Organizer: The 19th SANKEN International symposium
  • Place of Presentation: 大阪大学、吹田キャンパス
  • Year and Date: 2015-12-07
  • Int'l Joint Research
• [Presentation] Luminescent Ca2+ imaging in Marchantia polymorpha (2014)
  • Author(s): Megumi Iwano
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: Kobe University
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] 植物細胞における可視化技術の進展 (2014)
  • Author(s): 岩野 恵
  • Organizer: 日本顕微鏡学会 関西支部 平成26 年度 特別講演会プログラム
  • Place of Presentation: 兵庫県立大学（姫路工学キャンパス）
  • Year and Date: 2014-08-30
  • Invited
• [Book] 動植物の受精学 (2014)
  • Author(s): 岩野恵、土屋亨、掛田克行
  • Total Pages: 332
  • Publisher: 化学同人
• [Book] 動植物の受精学 (2014)
  • Author(s): 澤田均、岩野恵
  • Total Pages: 332
  • Publisher: 化学同人
• [Patent(Industrial Property Rights)] デバイス、およびそれを用いた判定システム (2017)
  • Inventor(s): 永井健治、新井由之、岩野恵
  • Industrial Property Rights Holder: 永井健治、新井由之、岩野恵
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas