Osmotic stress sensing and angiotensin II in body fluids homeostasis in amphibian CNS

• Project/Area Number: 26440164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Toyama
• Principal Investigator: UCHIYAMA Minoru 富山大学, 大学院理工学研究部(理学), 客員教授 (50095072)
• Co-Investigator(Kenkyū-buntansha): 今野 紀文 富山大学, 大学院理工学研究部(理学), 講師 (50507051)
• Research Collaborator: MUKUDA Takao ; HARUMI Tatsuo
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 両生類 / 体液恒常性 / 脳室周囲器官 / c-Fos / アンギオテンシン受容体 / 無尾両生類 / アンギオテンシンⅡ / AT1R免疫陽性ニューロン / c-Fos免疫陽性ニューロン / TRPVファミリー / ATR / 血管平滑筋 / c-fosタンパク / 吸水行動 / アンギオテンシンII

Outline of Final Research Achievements

The present study aimed to investigate forebrain areas and role of angiotensin II in body fluid homeostasis in amphibian brain. Circumventricular organs lack blood-brain barrier and probably function to detect changes of extracellular fluid. Although we cloned amphibian transient receptor potential vanilloid (TRPV) 1 and TRPV4 activated Ca++ permeability in response to osmotic stress in vitro, there was no significant change of TRPV-4 mRNA expression in dehydrated frogs using real-time quantitative PCR. C-Fos immunoreactive neurons were observed in several nuclei including the paraventricular nucleus (PVN) in forebrain of Japanese tree frog. The number of c-Fos immunoreactive cells increased in these nuclei of frogs under dehydrated condition. Angiotensin II type 1 receptor was immunohistochemically located in the medial septal nucleus (MSN). There may be physiological relationship between MSN and paraventricular nucleus according to analysis of the expression of c-Fos protein.

Research Products

• [Journal Article] アマガエルの雨鳴きの科学 (2017)
  • Author(s): 内山実
  • Journal Title: 両生類誌 Volume: 印刷中
  • Peer Reviewed
• [Journal Article] 両生類の体液調節 (2017)
  • Author(s): 内山実
  • Journal Title: 両生類誌 Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Urotensin II upregulates migration and cytokine gene expression in leukocytes of the African clawed frog, Xenopus laevis. (2015)
  • Author(s): Tomiyama, S., Nakamachi, T., Uchiyama, M., Matsuda, K., and Konno, N.
  • Journal Title: Gen. Comp. Endocrinol. Volume: in press Pages: 54-63
  • DOI: 10.1016/j.ygcen.2015.04.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cloning and expression of the epithelial sodium channel and its role in osmoregulation of aquatic and estivating African lungfish Protopterus annectens (2014)
  • Author(s): Uchiyama M., Konno N., Shibuya S., Nogami S.
  • Journal Title: Comparative Biochemistry and Physiology Volume: 183C Pages: 1-8
  • DOI: 10.1016/j.cbpa.2014.12.028
  • Peer Reviewed
• [Presentation] アマガエルの体液調節に関わる中枢神経系の解析 (2016)
  • Author(s): 内山実，椋田崇生，露谷孔明，前島翔，海谷啓之
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 神奈川県相模原市北里大学
  • Year and Date: 2016-12-10
• [Presentation] アマガエルの体液調節 (2016)
  • Author(s): 内山実
  • Organizer: 日本両生類研究会
  • Place of Presentation: 石川県近江町交流プラザ
  • Year and Date: 2016-08-27
• [Presentation] 水陸両生魚ヨダレカケの陸生適応機構 (2015)
  • Author(s): 内山実，小宮山牧子，上田雪絵，清水則雄，今野紀文
  • Organizer: 日本動物学会
  • Place of Presentation: 新潟コンベンションセンター 朱鷺メッセ
  • Year and Date: 2015-09-19
• [Presentation] 両生類における新規AQPの同定およびAQPの分子進化 (2015)
  • Author(s): 石井凌，内山実，鈴木雅一ほか8名
  • Organizer: 日本動物学会
  • Place of Presentation: 新潟コンベンションセンター 朱鷺メッセ
  • Year and Date: 2015-09-19
• [Presentation] Hormonal and osmotic regulation of water absorption behavior of Japanese tree frog, Hyla japonica (2014)
  • Author(s): Sho Maejima, Hiroyuki Kaiya, Norifumi Konno, Kouhei Matsuda and Minoru Uchiyama
  • Organizer: International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: National Institute for Basic Biology, Okazaki, Japan
  • Year and Date: 2014-11-09
  • Invited
• [Presentation] Ghrelin receptor in amphibians (2014)
  • Author(s): Hiroyuki Kaiya, Takio Kitazawa, Norifumi Konno, Minoru Uchiyama, Kenji Kangawa and Mikiya Miyazato
  • Organizer: International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: National Institute for Basic Biology, Okazaki, Japan
  • Year and Date: 2014-11-08
• [Presentation] 闘魚ベタの攻撃行動と脳内バソトシン発現との関連 (2014)
  • Author(s): 今野紀文，小池俊貴，岩田恵理，内山実，松田恒平
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2014-09-13
• [Presentation] アフリカ産肺魚のグレリン受容体 (2014)
  • Author(s): 海谷啓之，今野紀文，内山実，寒川賢治，宮里幹也
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2014-09-13
• [Presentation] カニクイガエルの汽水環境順化における体液浸透圧調節 (2014)
  • Author(s): 内山実，垣内祐貴，前嶋翔，杉浦駿
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2014-09-13
• [Presentation] ウロテンシⅡはアフリカツメガエルにおいて白血球遊走とサイトカイン合成に関与する (2014)
  • Author(s): 富山詩織，内山実，中町智哉，松田恒平，今野紀文
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2014-09-11
• [Presentation] アマガエルの雨鳴きを科学する (2014)
  • Author(s): 内山実
  • Organizer: 日本両生類研究会
  • Place of Presentation: 埼玉県立川の博物館
  • Year and Date: 2014-08-30
• [Book] ホルモンから見た生命現象と進化シリーズ 「ホメオスタシスと適応 ―恒―」 第1章 (2016)
  • Author(s): 海谷啓之，内山実
  • Publisher: 裳華房
• [Book] ホルモンから見た生命現象と進化シリーズ 「ホメオスタシスと適応 ―恒―」 第3章 (2016)
  • Author(s): 内山実
  • Publisher: 裳華房
• [Book] ホルモンから見た生命現象と進化シリーズ 「ホメオスタシスと適応 ―恒―」 第6章 (2016)
  • Author(s): 内山実
  • Publisher: 裳華房
• [Book] Angiotensin II and Water Balance in Amphibians/Sodium and Water Homeostasis (2015)
  • Author(s): Minoru Uchiyama
  • Publisher: Springer New York
• [Book] Angiotensin II and water balance in Amphibians In: Comparative, Evolutionary and Genetic Models of Sodium and Water Homeostasis (2015)
  • Author(s): Minoru Uchiyama
  • Publisher: Springer