Map-based cloning of salt tolerance gene and its utilization for improvement of salt tolerance in soybean
| Project/Area Number |
26450013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Japan International Research Center for Agricultural Sciences |
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Principal Investigator |
Xu Donghe 国立研究開発法人国際農林水産業研究センター, 生物資源・利用領域, 主任研究員 (90425546)
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| Co-Investigator(Kenkyū-buntansha) |
山田 哲也 北海道大学, 農学研究院, 講師 (70374618)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ダイズ / 耐塩性 / 遺伝子 / 単離 |
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| Outline of Final Research Achievements |
In this study, we isolated a salt tolerance gene (Ncl) using map-based cloning strategy. The salt-tolerant soybean lines showed higher expression of the salt tolerance gene Ncl in the root and resulted in lower accumulations of Na+, K+, and Cl- in the shoot under salt stress. We transformed Ncl into a soybean cultivar Kariyutaka using the Agrobacterium-mediated transformation method, and significantly enhanced its salt tolerance. To determine the usefulness of Ncl in conventional soybean breeding, we conducted introgression of Ncl into a salt-sensitive variety Jackson through continuous backcross, followed by DNA marker-assisted selection (MAS) in each generation, and finally produced an improved salt tolerance line. Our study suggests that using Ncl in soybean breeding through transgenic or MAS would contribute to sustainable soybean production in saline-prone areas.
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Report
(4 results)
Research Products
(4 results)
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[Journal Article] Ncl synchronously regulates Na+, K+, and Cl- in soybean and greatly increases the grain yield in saline field conditions2016
Author(s)
Tuyen Duc Do, Huatao Chen, Hien Thi Thu Vu, Aladdin Hamwieh, Tetsuya Yamada, Tadashi Sato, Yongliang Yan, Hua Cong, Mariko Shono, Kazuhiro Suenaga, Donghe Xu
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 19147-19147
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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