• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the mechanism leading to horizontal pollen transmission of raspberry bushy dwarf virus

Research Project

Project/Area Number 26450051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionIwate University

Principal Investigator

Isogai Masamichi  岩手大学, 農学部, 准教授 (30312515)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords花粉伝染 / 花粉による水平伝染 / 花粉による垂直伝染 / ラズベリー黄化ウイルス / 花粉管 / 胚のう / 柱頭 / リンドウ子房輪紋ウイルス / 植物ウイルス
Outline of Final Research Achievements

Mechanisms by which pollination with virus-contaminated pollen grains cause systemic viral infection to healthy plants have been unknown since 1918, when the possibility that viruses might be spread by pollen from plant to plant was first raised. This study has revealed that horizontal pollen transmission of raspberry bushy dwarf virus (RBDV) can result in infection of plants in families different from those of the pollen donor. In particular, RBDV can be horizontally transmitted to healthy plants by penetration of their stigmas by germinating pollen tubes originating from RBDV-infected plants. In addition, the study has also shown that RBDV-infected pollen tubes can introduce RBDV directly into embryo sacs during fertilization, and that this results in virus infection of seeds from the fertilized flowers.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (41 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results) Presentation (27 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Horizontal pollen transmission of Gentian ovary ring-spot virus is initiated during penetration of the stigma and style by infected pollen tubes2017

    • Author(s)
      Masamichi Isogai, Yukie Kamata, Syunpei Ando, Misaki Kamata, Asuka Shirakawa, Ken-Taro Sekine, Nobuyuki Yoshikawa
    • Journal Title

      Virology

      Volume: 503 Pages: 6-11

    • DOI

      10.1016/j.virol.2017.01.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Blueberry mosaic associated virus detected in a highbush blueberry tree with mosaic symptoms in Japan2016

    • Author(s)
      Masamichi Isogai, Kenshu Suzuki, Satoru Yashima, Manabu Watanabe, and Nobuyuki Yoshikawa
    • Journal Title

      Plant Virus Disease Workshop Report

      Volume: 12 Pages: 61-65

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a Raspberry bushy dwarf virus-based plant virus vector for virus-induced gene silencing2016

    • Author(s)
      Takuya Shimura, Tetu Yoshida, Nobuyuki Yoshikawa, and Masamichi Isogai
    • Journal Title

      Plant Virus Disease Workshop Report

      Volume: 12 Pages: 54-60

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Occurrence of blueberry mosaic associated virus in highbush blueberry trees with blueberry mosaic disease in Japan2016

    • Author(s)
      Isogai M., Matsuhashi Y., Suzuki K., Yashima S., Watanabe M., Yoshikawa N.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 印刷中 Issue: 3 Pages: 177-179

    • DOI

      10.1007/s10327-016-0653-z

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pollen tubes introduce Raspberry bushy dwarf virus into embryo sacs during fertilization process.2015

    • Author(s)
      Isagai M., Yoshida T., Shimura T., Yoshikawa N.
    • Journal Title

      Virology

      Volume: 484 Pages: 341-345

    • DOI

      10.1016/j.virol.2015.06.028

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Penetration of pollen tubes with accumulated Raspberry bushy dwarf virus into stigmas is involved in intial infection of maternal tissue and horizontal transmission2014

    • Author(s)
      Isogai M., Yoshida T., Nakanowatari C., Yoshikawa N.
    • Journal Title

      Virology

      Volume: 452-453 Pages: 247-253

    • DOI

      10.1016/j.virol.2014.02.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] リンゴ小球形潜在ウイルス(ALSV)ベクターで世代促進したリンドウ由来の後代実生からはALSV は検出されない2017

    • Author(s)
      鎌田和樹・山岸紀子・磯貝雅道・吉川信幸
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      アイーナ・いわて県民情報交流センター(岩手県盛岡市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラズベリー黄化ウイルス(RBDV)のゲノムにコードされる1b 遺伝子の機能解析2017

    • Author(s)
      松平昂士・志村拓哉・吉川信幸・磯貝雅道
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      アイーナ・いわて県民情報交流センター(岩手県盛岡市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 花粉管の柱頭への侵入はリンドウ子房輪紋ウイルスの花粉による水平伝染に関与している2016

    • Author(s)
      磯貝雅道・鎌田由希恵・安藤俊平・白川明日佳・関根健太郎・吉川信幸
    • Organizer
      日本植物病理学会
    • Place of Presentation
      岡山コンベンションセンター (岡山県・岡山市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Research-status Report
  • [Presentation] メガヌクレアーゼI-Sce I を発現する植物ウイルスベクターを用いた標的遺伝子への変異誘導2016

    • Author(s)
      野田浩気・笠島一郎・磯貝雅道・遠藤真咲・土岐精一・夏秋知英・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      コラッセふくしま(福島県福島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高温処理による感染ブドウ樹デのリンゴ小球形潜在ウイルス(ALSV)の増殖と全身移行の阻害2016

    • Author(s)
      前田清明・磯貝雅道・山下裕之・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      コラッセふくしま(福島県福島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] DNAマーカー7を利用したリンゴ品種の病害抵抗性および果実形質の評価2016

    • Author(s)
      菊池哲平・笠島一郎・山岸紀子・磯貝雅道・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      コラッセふくしま(福島県福島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] リンドウでのリンゴ小球形潜在ウイルスの種子伝染の調査2016

    • Author(s)
      鎌田和樹・山岸紀子・磯貝雅道・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      コラッセふくしま(福島県福島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] リンドウ子房輪紋ウイルスの花粉による垂直伝染の解析2016

    • Author(s)
      磯貝雅道・鎌田由希恵・安藤俊平・関根健太郎・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      コラッセふくしま(福島県福島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 花粉管によるラズベリー黄化ウイルスの胚のうへの導入2015

    • Author(s)
      磯貝雅道・吉田哲・志村拓哉・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      東北大学 (宮城県・仙台市)
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
  • [Presentation] リンゴモザイク病罹病樹での葉のモザイク症状とリンゴえそモザイクウイルス(ApNMV)分布の相関2015

    • Author(s)
      野田浩気・山岸紀子・八重樫元・磯貝雅道・伊藤伝・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      東北大学 (宮城県・仙台市)
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
  • [Presentation] リンゴ小球形潜在ウイルス(ALSV)ベクター技術を利用したブドウの開花促進2015

    • Author(s)
      前田清明・川崎未央子・佐藤誉子・門馬孝之・李春江・磯貝雅道・中島育子・山下裕之・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      東北大学 (宮城県・仙台市)
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Blueberry mosaic associated virus detected in a highbush blueberry tree with mosaic symptoms in Japan2015

    • Author(s)
      Isogai, M. Suzuki, K. Yashima, S. Watanabe, M. Yoshikawa N.
    • Organizer
      23rd International Conference on Viruses and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Morioka (Japan)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a Raspberry bushy dwarf virus-based plant virus vector for virus-induced gene silencing2015

    • Author(s)
      Shimura, T. Yoshida, T. Yoshikawa, N. Isogai, M.
    • Organizer
      23rd International Conference on Viruses and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Morioka (Japan)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multiple virus infection in cherry trees affected by a bud blight disease2015

    • Author(s)
      Yoshikawa, N. Oyamada, S. Yaegashi, H. Goto, S. Yamagishi, N. Isogai, M. Ito, T.
    • Organizer
      23rd International Conference on Viruses and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Morioka (Japan)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cherry virus B, a new virus classified into genus Foveavirus found in a sweet cherry tree2015

    • Author(s)
      Oyamada, S. Yaegashi, H. Goto, S. Yamagishi, N. Isogai, M. Ito, T. Yoshikawa, N.
    • Organizer
      23rd International Conference on Viruses and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Morioka (Japan)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ブルーベリー随伴ウイルスの日本分離株の全塩基配列2015

    • Author(s)
      八島理・渡邉学・吉川信幸
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] リンゴモザイク病に見いだされた新規イラルウイルス[リンゴえそモザイクウイルス, Apple necrotic mosaic virus (ApNMV)](仮称)について2015

    • Author(s)
      野田浩気・山岸紀子・八重樫元・磯貝雅道・伊藤伝・吉川信幸
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] ラズベリー黄化ウイルスのORF1bの欠失変異株の異種ウイルスのRNAサイレンシングサプレッサーによる相補試験2015

    • Author(s)
      志村拓哉・吉川信幸・磯貝雅道
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] リンゴ小球形潜在ウイルス(ALSV)ベクターによるオウトウおよびブドウでの外来遺伝子の発現2015

    • Author(s)
      前田清明・山岸紀子・李春江・磯貝雅道・五十鈴川寛司・山下裕之・田尾龍太郎・吉川信幸
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Horizontal transmission of Raspberry bushy dwarf virus by pollen2014

    • Author(s)
      Isogai M.
    • Organizer
      23th Annual Meeting & International Symposium on Plant Virus Diseases
    • Place of Presentation
      Pukyong National University, Busan, Korea
    • Year and Date
      2014-10-22
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 日本でのBlueberry mosaic associated virusの検出2014

    • Author(s)
      磯貝雅道・鈴木乾修・八島理・渡邉学・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ラズベリー黄化ウイルスのORF1bにコードされる12kDaタンパク質の機能解析2014

    • Author(s)
      志村拓哉・吉田哲・山岸紀子・吉川信幸・磯貝雅道
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      盛岡市 アイーナ
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] リンゴ小球形潜在ウイルス(ALSV)のオウトウ、モモおよびブドウ実生への感染とVIGSの誘導2014

    • Author(s)
      前田清明・八木皓平・山岸紀子・李春江・磯貝雅道・五十鈴川寛司・山下裕之・田尾龍太郎・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      盛岡市 アイーナ
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] モザイク症状を示すリンゴから分離された新規イラルウイルス(リンゴえそモザイクウイルス、Apple necrotic mosaic virus (ANMV))(仮称)について2014

    • Author(s)
      野田浩気・山岸紀子・八重樫元・磯貝雅道・伊藤伝・吉川信幸
    • Organizer
      日本植物病理学会東北部会
    • Place of Presentation
      盛岡市 アイーナ
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Penetration of pollen tubes with accumulated RBDV into stigma is essential in causing the first virl infection in the stigma to lead to systemic infection2014

    • Author(s)
      Isogai M., Yoshida T., Yoshikawa N., Nakanowatari C.
    • Organizer
      International Union of Microbiological Societies Congresses
    • Place of Presentation
      Palais des congres de Montreal, Montreal, Canada
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Research-status Report
  • [Presentation] ラズベリー黄化ウイルスの花粉による水平伝染の初期感染部位の解析2014

    • Author(s)
      吉田哲・吉川信幸・磯貝雅道
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 札幌コンベンションセンター2014

    • Author(s)
      志村拓哉・藤原早希・山岸紀子・吉川信幸・磯貝雅道
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Research-status Report
  • [Book] ひと目でわかる果樹の病害虫-第三巻(改訂第二版)2015

    • Author(s)
      坂神泰輔・工藤晟 編
    • Total Pages
      277
    • Publisher
      一般社団法人 日本植物防疫協会
    • Related Report
      2015 Research-status Report
  • [Book] 植物ウイルス辞典2015

    • Author(s)
      日比忠明・大木理 監修
    • Total Pages
      944
    • Publisher
      朝倉書店
    • Related Report
      2015 Research-status Report
  • [Book] CD-ROM版 日本植物病害大辞典 病害新情報2015

    • Author(s)
      岸國平・小林享夫・堀江博道 編
    • Publisher
      全国農村教育協会
    • Related Report
      2015 Research-status Report
  • [Book] 写真で見る東北の病害; ラズベリー黄化病2014

    • Author(s)
      磯貝雅道
    • Total Pages
      1
    • Publisher
      日本植物病理学会東北部会創立50周年記念誌刊行会
    • Related Report
      2014 Research-status Report
  • [Book] 写真で見る東北の病害; ブルーベリー赤色輪点病2014

    • Author(s)
      磯貝雅道
    • Total Pages
      1
    • Publisher
      日本植物病理学会東北部会創立50周年記念誌刊行会
    • Related Report
      2014 Research-status Report
  • [Remarks] How viruses hitch a ride on pollen

    • URL

      http://www.virologyhighlights.com/how-viruses-hitch-a-ride-on-pollen-to-infect-plants/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 植物的性病?!

    • URL

      http://pansci.asia/archives/58284

    • Related Report
      2016 Annual Research Report
  • [Remarks] 国立大学法人岩手大学 研究者総覧

    • URL

      http://univdb.iwate-u.ac.jp/profile.php?userId=28&lang_kbn=ja

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi