| Project/Area Number |
26450078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
木野内 忠稔 京都大学, 原子炉実験所, 講師 (90301457)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 植物 / ホウ素 / 細胞壁 / ストレス / ペクチン / 細胞死 / ラムノガラクツロナンII / KDO / 中性子捕捉反応 |
|---|
| Outline of Final Research Achievements |
Boron as an essential micronutrient for vascular plants occurs in cell walls, and cross-links pectin to form a gel. To get insights on physiological function of this gel in cell walls, we analyzed the phenotype of Arabidopsis mutant that was considered to be inhibited in biosynthesis of pectic RG-II, which is the site of borate-crosslinking. The mutant showed decreased cell division and elogation probably due to retarded formation of cell wall, but the mutant cell wall contained RG-II at a similar level to wild type, suggesting that defective cell wall without RG-II could never be formed. In addition, when RG-II was not properly cross-linked with B under B deficient condition, an cell death similar to hypersensitive cell death occurred immediately in the root elongation zone. These findings together demonstrate the physiological importance of boron-RG-II cross-linking.
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