Genetic engineering on increasing NADPH supply for reductive biotransformation by acetic acid bacteria.
| Project/Area Number |
26450095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松下 一信 山口大学, 創成科学研究科, 教授(特命) (50107736)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 酢酸菌 / 発酵 / 応用微生物 / 酵素 / バイオテクノロジー / 物質生産 / 還元反応 / NADPH / シキミ酸 / トランスヒドロゲナーゼ / 糖代謝 |
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| Outline of Final Research Achievements |
Gluconobacter sp., acetic acid bacterium, oxidizes glucose on the outer surface of the cytoplasmic membrane with membrane-bound glucose dehydrogenase (GdhM) and accumulates gluconic acid and its derivatives. Thus, only limited parts of glucose are transported into the cytoplasm and metabolized. Because glucose metabolism in the cytoplasm mostly depends on pentose phosphate pathway, it is reasonably assumed that G. oxydans likely produces much NADPH, if high amounts of glucose are metabolized in the cytoplasm. This study aimed to increase NADPH supply from glucose by inactivating GdhM. This study tried to couple NADPH produced in the ΔgdhM cells and the reduction reaction producing shikimate from dehydroshikimate. When cultivated on glucose, biomass yield and amounts of an end metabolite in the ΔgdhM strain were elevated. Taken together with enzyme activity measurements, some changes on glucose metabolism in the ΔgdhM cells suggested increases in the NADPH level.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, <i>Acetobacter pasteurianus</i> SKU1108, Provide a New Insight into Thermotolerance2016
Author(s)
Matsutani, M., Hirakawa, H., Hiraoka, E., Theeragool, G., Yakushi, T. and Matsushita, K.
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Journal Title
Microbes and Environments
Volume: 31
Issue: 4
Pages: 395-400
DOI
NAID
ISSN
1342-6311, 1347-4405
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains.2016
Author(s)
Sainz, F., Jesus Torija, M., Matsutani, M., Kataoka, N., Yakushi, T., Matsushita, K. and Mas, A.
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Journal Title
Frontiers in Microbiology
Volume: 7
Pages: 1358-1358
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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