The regulatory mechanisms of the induction of lipase expression and polyester production by stearyl alcohol

• Project/Area Number: 26450101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Rikkyo University (2015-2016); Chuo University (2014)
• Principal Investigator: AKANUMA Genki 立教大学, 理学部, 助教 (30580063)
• Co-Investigator(Kenkyū-buntansha): 石塚 盛雄 中央大学, 理工学部, 教授 (50168241)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: リパーゼ / ポリエステル / Ralstonia / Ｌｉｐａｓｅ / ＰＨＢ / Ｒａｌｓｔｏｎｉａ / Lipase / EliA / PHB

Outline of Final Research Achievements

The transcriptional factor which activates the transcription of gene encoding lipase was determined. Moreover, we found the alcohol dehydrogenase which plays important role for induction of lipase expression by stearyl alcohol. The EliA, a secretory protein, is essential for the lipase-producing bacterium to respond to stearyl alcohol.

Research Products

• [Journal Article] EliA is required for inducing the stearyl alcohol-mediated expression of secretory proteins and production of polyester in Ralstonia sp. NT80. (2016)
  • Author(s): Akanuma G, Yoshizawa R, Nagakura M, Shiwa Y, Watanabe S, Yoshikawa H, Ushio K, Ishizuka M.
  • Journal Title: Microbiology Volume: 162 Issue: 2 Pages: 408-419
  • DOI: 10.1099/mic.0.000225
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis. (2016)
  • Author(s): Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, Hanai R, Nanamiya H, Kato-Yamada Y, Kawamura F.
  • Journal Title: Microbiology Volume: 162 Issue: 3 Pages: 448-458
  • DOI: 10.1099/mic.0.000234
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium. (2014)
  • Author(s): Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M.
  • Journal Title: Ｊｏｕｒｎａｌ ｏｆ Ｂａｃｔｅｒｉｏｌｏｇｙ Volume: 196 Issue: 22 Pages: 3820-3830
  • DOI: 10.1128/jb.01896-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Ralstonia sp. NT-80 株におけるリパーゼ誘導促進因子の機能解析 (2016)
  • Author(s): 千濱良太、石橋隼、粟井貴子、赤沼元気、牛尾一利、石塚盛雄
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Ralstonia sp. NT-80株におけるリパーゼ 転写調節因子LipR80-4の機能解析 (2016)
  • Author(s): 多勢 真大、関谷 麻美、赤沼 元気、石塚 盛雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌）
  • Year and Date: 2016-03-29
• [Presentation] Ralstonia sp.NT-80株におけるリパーゼ転写調節因子LipR80-4の機能解析 (2015)
  • Author(s): 多勢 真大、関谷 麻美、赤沼 元気、石塚 盛雄
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] 高級アルコールに対する微生物の反応 (2014)
  • Author(s): 赤沼 元気、吉澤 梨絵、永倉 茉莉、大塚 拓、志波 優、渡辺 智、吉川 博文、牛尾 一利、石塚 盛雄
  • Organizer: グラム陽性菌ゲノム機能会議
  • Place of Presentation: 山形県鶴岡市
  • Year and Date: 2014-09-03 – 2014-09-05