| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Serine is a typical dispensable amino acid and known essentiality of its de novo synthesis. To clarify the metabolic demand of dispensable amino and its de novo synthesis, serine concentration and expression of PHGDH, the rate limiting enzyme of serine synthesis, was determined in several tissues of rat under different nutritional conditions and diabetes. The result shows that the protein nutrition most significantly affected the serine concentrations in tissues and plasm. It is found that serine concentration of liver, intestine and muscle was significantly decreased in diabetes rat. PHGDH was demonstrated to express ubiquitously in tissues investigated and the expression in liver, intestine and muscle was changed in response to protein nutrition. However, the clear relation between the expression of PHGDH and serine concentrations of tissues was not demonstrated.
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